We narrowed to 659 results for: Clu
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TypeBlog PostPublishedApril 8, 2025, 1:15 p.m....axis. This effect causes misleading qualitative conclusions, and it’s also problematic for computational ...
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Hot Plasmids: Summer 2024
TypeBlog PostPublishedAug. 27, 2024, 1:15 p.m....variants deposited with Addgene. These variants, including a “miniCRISPR” version, are much smaller proteins... -
CRISPR 101: Homology Directed Repair
TypeBlog PostPublishedJan. 26, 2023, 2:15 p.m....SDSA is a conservative repair mechanism which exclusively yields non-crossover events. This means all newly... -
Deep Mutational Scanning with One Pot Saturation Mutagenesis
TypeBlog PostPublishedFeb. 22, 2017, 3:30 p.m....addition of MnCl2. Disadvantages of this method include favoring of certain types of mutations, difficulty... -
Site Directed Mutagenesis by PCR
TypeBlog PostPublishedAug. 2, 2016, 2:30 p.m.... sequencing functional regions of the plasmid including the insert. This is essential since mutations ... -
Evolution of Lab Techniques
TypeBlog PostPublishedJune 21, 2016, 2:30 p.m....high-quality DNA, real-time PCR has several advantages including easier automation, higher throughput screening... -
Treating Muscular Dystrophy with CRISPR Gene Editing
TypeBlog PostPublishedJan. 26, 2016, 3:30 p.m.... genetic diseases caused by splicing defects, including ataxia telangiectasia, congenital disorder of ... -
Technologies Enabled by NanoLuc® Luciferase
TypeBlog PostPublishedFeb. 8, 2018, 12:17 p.m....of applications of the Enhanced Nano-Lanterns including monitoring multiple cellular events such as the... -
Antibodies 101: Multiplex Immunofluorescence
TypeBlog PostPublishedMay 23, 2023, 1:15 p.m.... images once taken. To do so, you will need to include a marker like the Hoechst stain for the nuclei.... -
Plasmids 101: Dimers and Multimers
TypeBlog PostPublishedJan. 10, 2023, 2:15 p.m....plasmid sequencing services to verify the plasmids, including long-read sequencing techniques, such as Oxford... -
Technique: Probe Phage Genomes for Host Binding Proteins
TypeBlog PostPublishedSept. 29, 2016, 2:30 p.m....refer to the paper for more details on the assay, including examples of RBPs successfully identified using... -
Recombinase-based State Machines Enable Order-dependent Logic in vivo
TypeBlog PostPublishedJuly 28, 2016, 2:30 p.m....recombinase-specific co-factors (which should be excluded from the system), these reactions are irreversible... -
Generating Mouse Models Using CRISPR/Cas9
TypeBlog PostPublishedJuly 12, 2016, 2:30 p.m....targeting, which is limited to a few strains, including 129 and C57BL/6, for which we have germline competent... -
Plasmids 101: Restriction Cloning
TypeBlog PostPublishedFeb. 18, 2016, 3:42 p.m....visualize the DNA in your gel (this table is not inclusive of all gel stains): Stain Pre or Post Run?... -
FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution
TypeBlog PostPublishedOct. 17, 2019, 12:28 p.m....Microbial rhodopsins are encoded by opsin genes, and include bacteriorhodopsins, halorhodopsins, and channelrhodopsins... -
Transferable Skills Guide: Teamwork
TypeBlog PostPublishedNov. 28, 2017, 2 p.m....helped assemble a crack team of Addgenies that includes: Michelle, the Senior Scientist who coordinates... -
Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins
TypeBlog PostPublishedApril 25, 2017, 2:30 p.m....have helped create several new imaging methods, including photoactivation-localization microscopy (PALM,... -
Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation
TypeBlog PostPublishedNov. 6, 2018, 1:12 p.m....methods. Some of the more common delivery methods include retrovirus [2], lentivirus [4], adenovirus [5],... -
CRISPR 101: Cytosine and Adenine Base Editors
TypeBlog PostPublishedFeb. 13, 2025, 2:15 p.m....groups have made improvements to base editors, including: expanding targeting scope improving editing ...