We narrowed to 801 results for: Cre
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TypeBlog PostPublishedMarch 8, 2021, 2:41 p.m....proteins, use a higher percentage of acrylamide to increase migration resistance and improve separation. For...enhance resolution. In this setup, gels have an increasing range of acrylamide content from top to bottom...
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Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein
TypeBlog PostPublishedJune 28, 2018, 3:01 p.m....antibody” can be used for downstream IP/ChIP assays. Creating epitope-tagged clonal cell lines remains a challenge...crRNA complementary to the template strand could increase knock-in efficiency.Note: Our TAG-IN tool can ... -
A Needle in a Base-Stack: Cas9 Structural Biology
TypeBlog PostPublishedJune 4, 2024, 1:15 p.m...., while REC includes most of the bridge helix. Created with BioRender.com. Cas9 has two major lobes...guide RNA, dark blue; DNA, gray; PAM site, yellow. Created with BioRender.com. The exact conformational... -
How to Deposit Your Plasmids with Addgene
TypeBlog PostPublishedFeb. 27, 2024, 2:15 p.m....process. To deposit plasmids online, you will need to create an account with Addgene before starting the process...plasmids, and adding all the plasmids at once increases the likelihood that the plasmids are given sequential... -
Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs
TypeBlog PostPublishedDec. 20, 2017, 2:33 p.m.... cancer and your continued work is necessary to create therapeutics to cure the cancer. Authorship: Publishing...translations of documents that are not in English. Create a Google Scholar account Sanitize your LinkedIn... -
'Tis the Season to #DeckTheLab
TypeBlog PostPublishedDec. 2, 2016, 3:30 p.m....impressive entries, but we have faith our community of creative scientists will deliver some fabulous photos again... -
CRISPR Guide Update
TypeBlog PostPublishedOct. 10, 2024, 1:15 p.m....added new sections to share and describe some incredible new CRISPR technologies developed and widely ... -
Addgene's 20th Anniversary Party
TypeBlog PostPublishedJune 11, 2024, 1:15 p.m....can't celebrate Addgene without recognizing our incredible depositors and requestors. Thank you all so much... -
The 12 Days of CRISPR
TypeBlog PostPublishedJan. 3, 2023, 2:15 p.m....gave to me: CRISPR activation and interference screens for primary human T cells🎵 On the 3rd day of CRISPR... -
New FLIS Plasmid (April Fools 2022)
TypeBlog PostPublishedApril 1, 2022, 1:15 p.m....interrogate many scientific questions. But these tiny creatures do tend to come with a rather astonishing ability... -
We’re Going Viral This Week!
TypeBlog PostPublishedOct. 14, 2019, 1 p.m....came out last year on sucrose supplementation to increase AAV2 yields and we’re eager to share more! Lastly... -
Overwhelmed? Take a Break With Our 5 Favorite Science Comics
TypeBlog PostPublishedDec. 5, 2013, 3:27 p.m.... showed that a laughter program significantly decreased immunological markers of stress in adolescents... -
Twenty Years of Sharing Science!
TypeBlog PostPublishedJan. 8, 2024, 3:25 p.m...., Korea, Japan, India, and Brazil. We've also created a robust support system for scientists using materials... -
New Videos: Addgene Lab Tips
TypeBlog PostPublishedFeb. 15, 2018, 2:41 p.m....Addgene Videos Page 5 Science Rap Videos and Their Creator: Tom McFadden Using Video to Share Your Science... -
Addgene’s GIPHY Page is Here to Help You Express Yourself (and Your Gene of Interest)
TypeBlog PostPublishedAug. 17, 2021, 1:15 p.m....answers! via GIPHY We’ve been working hard to create the most informative and entertaining protocol ... -
Twelve Amazing Guest Blog Contributors!
TypeBlog PostPublishedNov. 26, 2024, 2:15 p.m....’s lab. X-Chime and Performing In Vivo CRISPR Screens Using the FITS Approach Caitlin Faulds, who introduced... -
DIY DNA Ladders from Penn State University
TypeBlog PostPublishedJuly 14, 2017, 2:30 p.m....kb ladder. Using a commercial plasmid prep kit increases the costs to $20-30 per ladder: still significantly... -
Top Requested Plasmid of 2017 - pMD2.G
TypeBlog PostPublishedJan. 10, 2018, 4:13 p.m.... This plasmid has been used in studies of the secretome, ubiquitin ligase, cellular senescence, autophagy... -
Bringing Renewable Energy to Addgene
TypeBlog PostPublishedAug. 27, 2019, 1:08 p.m....early July the Town of Watertown announced the creation of an “electricity aggregation program” for all... -
Our New Antibody Mascot is....
TypeBlog PostPublishedFeb. 24, 2022, 2:15 p.m....a plethora of options, ranging from Affie to Yncredibody. After an agonizing week spent in cloistered ...