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We narrowed to 807 results for: Cre

Showing: 641 - 660 of 807 results
  1. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ... S65T) in the original jellyfish GFP (AvGFP) to create enhanced GFP (EGFP), a variant with improved brightness...fluorescent probes, recent engineering efforts have increased the brightness of these genetically encoded light...
  2. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    Published
    Nov. 16, 2021, 2:15 p.m.
    ...immunofluorescence and indirect immunofluorescence work. Image created with BioRender.com   Indirect IF is a much ...with key considerations about each step. Image created with BioRender.com   The first couple of steps...
  3. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ...fluorescent proteins (NIR FPs) and biosensors have been created recently. As developers of many of these probes..., A.A., Oliinyk, O.S. & Verkhusha, V.V. How to Increase Brightness of Near-Infrared Fluorescent Proteins...
  4. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ...A., Tao, Y. A., Duby, J. E., Steinbeck, B. J., McCreary, J., Pierce, S. E., … & Liu, D. R. (2024). Efficient...variety of DNA-targeting proteins, so the team created versions using dCas9, zinc finger proteins, and...
  5. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ... (Jambor et al., 2021) for more guidelines for creating clear and informative figures. Pro-tip! If you..., Wissink, E. M., & Weissgerber, T. L. (2021). Creating clear and informative image-based figures for ...
  6. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    Published
    March 8, 2021, 2:41 p.m.
    ...proteins, use a higher percentage of acrylamide to increase migration resistance and improve separation. For...enhance resolution. In this setup, gels have an increasing range of acrylamide content from top to bottom...
  7. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    Published
    Feb. 27, 2024, 2:15 p.m.
    ...process. To deposit plasmids online, you will need to create an account with Addgene before starting the process...plasmids, and adding all the plasmids at once increases the likelihood that the plasmids are given sequential...
  8. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    Published
    June 4, 2024, 1:15 p.m.
    ..., while REC includes most of the bridge helix. Created with BioRender.com.   Cas9 has two major lobes...guide RNA, dark blue; DNA, gray; PAM site, yellow. Created with BioRender.com.   The exact conformational...
  9. 'Tis the Season to #DeckTheLab

    Type
    Blog Post
    Published
    Dec. 2, 2016, 3:30 p.m.
    ...impressive entries, but we have faith our community of creative scientists will deliver some fabulous photos again...
  10. CRISPR Guide Update

    Type
    Blog Post
    Published
    Oct. 10, 2024, 1:15 p.m.
    ...added new sections to share and describe some incredible new CRISPR technologies developed and widely ...
  11. The 12 Days of CRISPR

    Type
    Blog Post
    Published
    Jan. 3, 2023, 2:15 p.m.
    ...gave to me: CRISPR activation and interference screens for primary human T cells🎵 On the 3rd day of CRISPR...
  12. Addgene's 20th Anniversary Party

    Type
    Blog Post
    Published
    June 11, 2024, 1:15 p.m.
    ...can't celebrate Addgene without recognizing our incredible depositors and requestors. Thank you all so much...
  13. We’re Going Viral This Week!

    Type
    Blog Post
    Published
    Oct. 14, 2019, 1 p.m.
    ...came out last year on sucrose supplementation to increase AAV2 yields and we’re eager to share more! Lastly...
  14. New FLIS Plasmid (April Fools 2022)

    Type
    Blog Post
    Published
    April 1, 2022, 1:15 p.m.
    ...interrogate many scientific questions. But these tiny creatures do tend to come with a rather astonishing ability...
  15. New Videos: Addgene Lab Tips

    Type
    Blog Post
    Published
    Feb. 15, 2018, 2:41 p.m.
    ...Addgene Videos Page 5 Science Rap Videos and Their Creator: Tom McFadden Using Video to Share Your Science...
  16. Twenty Years of Sharing Science!

    Type
    Blog Post
    Published
    Jan. 8, 2024, 3:25 p.m.
    ..., Korea, Japan, India, and Brazil.  We've also created a robust support system for scientists using materials...
Showing: 641 - 660 of 807 results