We narrowed to 811 results for: Cre

...interest is toxic. To combat this, scientists have created synthetic promoters, which typically include some...with somewhat weak expression. lacIq mutation increases expression of the repressor 10x, thus tightening...

... S65T) in the original jellyfish GFP (AvGFP) to create enhanced GFP (EGFP), a variant with improved brightness...fluorescent probes, recent engineering efforts have increased the brightness of these genetically encoded light...

...immunofluorescence and indirect immunofluorescence work. Image created with BioRender.com   Indirect IF is a much ...with key considerations about each step. Image created with BioRender.com   The first couple of steps...

...fluorescent proteins (NIR FPs) and biosensors have been created recently. As developers of many of these probes..., A.A., Oliinyk, O.S. & Verkhusha, V.V. How to Increase Brightness of Near-Infrared Fluorescent Proteins...

...plus one additional split vector strategy for increasing the size of the AAV donor template for CRISPR-mediated... AAV vector approaches Split vector approaches increase the size of the gene delivered by splitting the...

...completing the research will advance the field, create a new field, impact disease treatment, etc., again... His interests include the quality control of secretory proteins in the Endoplasmic Reticulum, optimization...

...A., Tao, Y. A., Duby, J. E., Steinbeck, B. J., McCreary, J., Pierce, S. E., … & Liu, D. R. (2024). Efficient...variety of DNA-targeting proteins, so the team created versions using dCas9, zinc finger proteins, and...

...proteins, use a higher percentage of acrylamide to increase migration resistance and improve separation. For...enhance resolution. In this setup, gels have an increasing range of acrylamide content from top to bottom...

... (Jambor et al., 2021) for more guidelines for creating clear and informative figures. Pro-tip! If you..., Wissink, E. M., & Weissgerber, T. L. (2021). Creating clear and informative image-based figures for ...

...antibody” can be used for downstream IP/ChIP assays. Creating epitope-tagged clonal cell lines remains a challenge...crRNA complementary to the template strand could increase knock-in efficiency.Note: Our TAG-IN tool can ...

...process. To deposit plasmids online, you will need to create an account with Addgene before starting the process...plasmids, and adding all the plasmids at once increases the likelihood that the plasmids are given sequential...

..., while REC includes most of the bridge helix. Created with BioRender.com.   Cas9 has two major lobes...guide RNA, dark blue; DNA, gray; PAM site, yellow. Created with BioRender.com.   The exact conformational...

... cancer and your continued work is necessary to create therapeutics to cure the cancer. Authorship:  Publishing...translations of documents that are not in English. Create a Google Scholar account Sanitize your LinkedIn...

...impressive entries, but we have faith our community of creative scientists will deliver some fabulous photos again...

...added new sections to share and describe some incredible new CRISPR technologies developed and widely ...

...gave to me: CRISPR activation and interference screens for primary human T cells🎵 On the 3rd day of CRISPR...

...can't celebrate Addgene without recognizing our incredible depositors and requestors. Thank you all so much...

...came out last year on sucrose supplementation to increase AAV2 yields and we’re eager to share more! Lastly...

...interrogate many scientific questions. But these tiny creatures do tend to come with a rather astonishing ability...

... showed that a laughter program significantly decreased immunological markers of stress in adolescents...