We narrowed to 694 results for: EED
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TypeBlog PostPublishedOct. 22, 2014, 1:57 p.m....microphone system ready for each panelist if the room needs it (lapel mics are best). The moderator takes control...
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Quick Guide to Working with Drosophila Part 1: Getting Started with Flies
TypeBlog PostPublishedJuly 13, 2017, 2:30 p.m....as homozygous alleles, others cannot. Geneticists need a method for maintaining stable lines of genetic... -
Truncated gRNAs for Regulating Gene Expression
TypeBlog PostPublishedJan. 10, 2017, 3:37 p.m....already express nuclease-competent Cas9, obviating the need to generate new cell lines or transgenic animals... -
The Scientific Conference Poster Session: Tips for Success
TypeBlog PostPublishedAug. 29, 2019, 12:50 p.m....Your colleagues, lab mates, and PI can provide feedback on your technical presentation, but those outside... -
Tips to Make the Most of a Scientific Conference
TypeBlog PostPublishedMay 23, 2019, 12:26 p.m....during the conference, you can try getting up to speed on social media. If you haven’t already, connect... -
Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria
TypeBlog PostPublishedApril 4, 2019, 12:53 p.m....subtilis (ICEBs1) system. ICEBs1 carries the genes needed for conjugation, but can also carry cargo genes... -
Hot Plasmids: Winter 2025
TypeBlog PostPublishedMarch 11, 2025, 1:15 p.m....the potential to improve rapid and precise plant breeding. Find dicot prime editing plasmids here! Vu, ... -
Degrading DNA with Cascade-Cas3
TypeBlog PostPublishedFeb. 11, 2025, 2:15 p.m....continued. However, Cas3 cannot bind DNA on its own and needs to remain anchored to Cascade throughout the process... -
The AAVantages of AAV in CRISPR Screens
TypeBlog PostPublishedFeb. 4, 2025, 2:15 p.m....Cas effectors in specific tissues, eliminating the need to deliver the Cas protein altogether. Platforming... -
Twenty Years of Addgene Sharing: CRISPR
TypeBlog PostPublishedApril 18, 2024, 1:15 p.m.... Addgene depositors make CRISPR tools that fill needs in the community. The rapid adoption of new tools... -
PhD Applications After COVID
TypeBlog PostPublishedJan. 5, 2023, 2:15 p.m....your nerves and make sure you cover everything you need to during the interview. (Virtual) Campus Visits... -
Hot Plasmids and Viral Preps - May 2021
TypeBlog PostPublishedMay 18, 2021, 1:15 p.m.... easier for you to find and use the plasmids you need. Here's what you'll find in this post: Pooled library... -
3D Printing Meets CRISPR Cas9
TypeBlog PostPublishedOct. 17, 2017, 12:55 p.m....compelling, and I encourage you to hold one. Why do we need physical models of protein structure? Structural... -
PITChing MMEJ as an Alternative Route for Gene Editing
TypeBlog PostPublishedFeb. 23, 2016, 3:30 p.m....created in about a month and a half, without the need for complicated cloning of homology arms. MMEJ... -
CRISPR 101: Ribonucleoprotein (RNP) Delivery
TypeBlog PostPublishedSept. 6, 2018, 12:02 p.m.... and stability of the Cas9 protein in the host. Indeed, research has shown that a Cas9 protein from Geobacillus... -
AAVs in Retinal Gene Therapy
TypeBlog PostPublishedJuly 31, 2018, 12:55 p.m.... systemic delivery of high doses of vectors are needed. Additionally, AAVs do not integrate in the host... -
Plasmids 101: Secondary Nanobody Toolbox
TypeBlog PostPublishedFeb. 27, 2018, 2:04 p.m....Bacteria: Table 2 also has a link to the plasmid you’ll need to express your nanobody of choice in bacteria. ... -
AAVs CREATed for Gene Delivery to the CNS and PNS
TypeBlog PostPublishedSept. 28, 2017, 2:01 p.m....transgene into a specific cell type in the brain, you needed to make a transgenic mouse. Combining AAv.PHP.eB... -
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog PostPublishedAug. 27, 2020, 1:15 p.m....Flexible GS linkers may also work. You will also need to generate negative control constructs — more on... -
Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus
TypeBlog PostPublishedMarch 17, 2016, 2:30 p.m....promoter sequence. Thus, T7 RNA polymerase also needs to be present in the cells to transcribe the viral...