We narrowed to 859 results for: ELL

...mashed potato bar (complete with all the fixings), as well as plenty of delicious desserts. We enjoyed a video...the changes at Addgene over the last 10 years as well as the many people that make up the Addgene family...Anniversary Gala. The Addgene lab often works as a very well-oiled machine and this post was no exception - the...

...21st century and all. But while the auto-reply telling you your message was received is a nice touch, ...answer your question. Does this sound familiar? Well, when it comes to working with Addgene, we certainly...and requesting them from your colleagues, to intellectual property rights and handling your constructs...

... modified Ti plasmids during plant infection, as well as plasmid yield during preparation in E. coli. ...both β-glucuronidase (GUS) and GFP reporters, as well as multiple transient and transgenic systems, Sahoo...versatile gene expression vector for transient assays as well as stable expression of foreign genes in plants....

...give you a chance at an interview:  1) Have an excellent resume ready to be submitted The structure and...successful application. It takes time to prepare an excellent resume…it is worth it. Read here to get help if...if you are having trouble preparing your most excellent resume. 2) Follow job boards and check them regularly...

...they were barely effective at editing eukaryotic cells — at least, until they met the directed evolution...components. It drove 19% integration in HEK293T cells (more than 500 times better than the precursor it...R. (2025). Programmable gene insertion in human cells with a laboratory-evolved CRISPR-associated transposase...

...Fluorescence Activated Cell Sorting (FACS), allowing for enrichment of successfully targeted cells that can be used...investigating genetic mechanisms related to morphogenesis, cell fate specification, and human disease.     Sun,...

...predict that we’d be able to edit RNA in mammalian cells with CRISPR? I certainly didn’t, but now we can ...development of CRISPR into a varied research toolbox, as well as additional resources to help you Plan Your Experiment...Improve HDR Efficiency for CRISPR Editing in Human Cells Day 3: Cpf1: Cpf1: A New Tool for CRISPR Genome ...

...precise mechanistic details of the process are not well understood, it is believed that the exonuclease ...properly. Directly transform chemically competent cells with the digested mixture. There is no need for ...purification before transformation. After transforming cells, you should perform colony PCR with vector specific...

...paired with patient partners so that they can help tell their stories. I was paired with a six-year-old ...similar age. When he came to visit us and watch fellow Run4Rare team members complete the Boston Marathon... scientific community know, treatments that work well in animal models do not always work as efficiently...

...you feel about dissecting a cat?' And I’m like, 'Well, I’m here so let’s do it!'" Working as a subject...inside. We do a lot of interactives and videos, as well, to supplement the hands-on [aspect of the labs]...interviewed, ‘I write science. I edit science. And I do it well.’” Stay tuned for the next feature in this series...

... a range of different scientific disciplines, as well as social and networking events. Imperial Synthetic... networking opportunities for society members as well as arranging collaborations and conferences for ...believe it’s very important to develop a network of fellow scientists that you can grow with, learn from, ...

...'t all career skills leaderhsip skills when done well? Do you just have to know it when you see it? “The...Managers must ensure that their teams are working well together and collaborating effectively with other...the doing. Many science managers or leaders will tell you how much they miss the hands-on bench work. ...

...will have opportunities to work on Emotional Intelligence or to hone delegation skills, for example. So...their managers. You can’t just think it, you have to tell them. A good manager learns that her success and...dissatisfaction, the work can get done, and get done well. The rest of the blogs in this series will address...

...the world.  Nina Dudnik, Founder of Seeding Labs, tells the story about working as a student in a rice lab...I was in graduate school in the 80’s, my lab was well funded and we didn’t feel the need to reuse plasticware...recently shared our protocol for making competent cells to save time and money. My other stingy science ...

...Consider reading Toxic Academic Mentors by @drmellivora, an excellent blog post about workplace bullying featured...the first place. The good news There are many excellent mentors out there. With some good pre-work you...

... road, science communication graduates are often well-equipped. Many institutions are sincerely interested...carried over and allowed to percolate within a new intellectual environment. Over time, this interest came to...risk-taking behavior among collision sport athletes, as well as the ethical and health policy implications arising...

...fully open and available to industry requestors as well. This is a time when we need as much open science...accelerates and enhances productive collaborations as well as provides visibility for grant-driven research...scientists worldwide with Addgene’s traditional excellence through whatever lies ahead this year. We can...

...Academic Solos to Industrial Symphonies, an article well worth reading on this topic, the authors state, ...colleagues and friends why he would give up,“an excellent—some say “cushy”—tenured faculty position for ...especially good at self-promoting—No one is going to tell you to pick up the pace Constant worry about grants...

...the number of added biotins per molecule cannot be well-controlled. The lack of site selectivity typically...successfully used this plasmid to biotinylate the extracellular domain of the erythropoietin receptor and subsequently... Eye Institute and is particularly interested in cell signaling.     Additional Resources on the Addgene...

...scientist at MIT’s Computer Science and Artificial Intelligence Lab and a Visiting Professor at the Lab for ...Classifying genes as natural or synthetic But how can you tell if a gene is synthetic or natural? The answer to...distantly related organism, the gene may not express well because of these different codon usage biases. But...