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We narrowed to 930 results for: Gene

Showing: 641 - 660 of 930 results

  1. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    Published
    July 6, 2017, 1:59 p.m.
    ...Additional Resources on the Addgene Blog Read Additional Cancer Blog Posts Addgene's Cancer Collection Pages...this multi-pronged attack. Inflammation and tumorigenesis The conference opened with a very interesting... between tumor and immune cells in real time. Addgene depositor Professor Max Krummel from UCSF showed...engineering (Ref 5)  as described by Blue Flame Addgene depositor Professor Michel Sadelain from the Memorial...poster is pTomo-Ras which has been deposited at Addgene was used to produce the glioblastoma model for ...addition, it was exciting to see that the use of optogenetic tools is expanding to immunological research....with the scientific community, contact deposit@addgene.org to find out more about our free deposit and distribution...

  2. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    Published
    Dec. 11, 2018, 12:46 p.m.
    ...Before I started writing for the Addgene blog, sharing Chemistry Cat memes was how I used social media...social media as a scientist. Now I write for the @Addgene blog & I'm my lab's resident #scicomm expert. What...using social media as a scientist started with the Addgene blog. I wrote my first blog post out of boredom...person in the lab. When I saw a request for an Addgene guest blogger post about colony PCR, I signed up...and accepted a position to work remotely as an Addgene Science Communication Intern. Developing science... using Twitter, I found this paper. I wrote an Addgene blog post about it and shared the paper with my... this panel, but my consistent writing for the Addgene blog (~1-2 blog posts per month) and use of Twitter...

  3. Expanded Access to Rabies Plasmids

    Type
    Blog Post
    Published
    Feb. 1, 2024, 2:15 p.m.
    ...Addgene is excited to announce we now offer an export license application for rabies materials! Some ... export-controlled materials in the USA, where Addgene is located.  The export license, if granted, allows...ship rabies plasmids and viral vectors containing genetic elements from rabies plasmids to international ... export control license, once approved, allows Addgene to ship plasmids or viral vectors containing sequences...project when purchasing export-controlled materials. Addgene will then apply for the license for distribution...processing costs. Once the application is approved, Addgene then ships the material following our normal process... If you have any questions about this or other Addgene shipping and ordering processes, don’t hesitate...

  4. Imaging Tools and Following a Passion for Basic Biology: Interview with Joachim Goεdhart

    Type
    Blog Post
    Published
    March 9, 2018, 2 p.m.
    ...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.      Additional... Today on the Addgene Podcast, we have another interview conducted by European Outreach Scientist Benoit...creation of improved fluorescent proteins through mutagenesis. Chapter 6 - The future of FRET and fluorescent...Amsterdam). He develops, characterizes and uses genetically encoded fluorescent probes. You can follow him...Additional Resources from the Addgene Blog Visit our Fluorescent Proteins featured topic page Read Joachim's...Subcellular Structures and Organelles Resources at Addgene.org Find Fluorescent Protein Plasmids Find Plasmids... addgene podcast...

  5. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    Published
    July 14, 2021, 1:15 p.m.
    ...plasmid-based antibodies Resources on Addgene.org Discover Addgene’s antibody plasmid collection ...antibodies are produced Polyclonal antibodies are a heterogeneous mixture of many antibodies that recognize the... response.   Polyclonal antibodies are generated by injecting an animal with an immunogen, then...weeks later. Created with biorender.com. To generate polyclonal antibodies, an animal (such as a rabbit...might be the full-length protein that you want to generate antibodies against, or a unique peptide sequence...experiments to be aware of signal strength. In general, polyclonal antibodies produce a higher level of...the same species your polyclonal antibody was generated in or a polyclonal isotype control, to account...

  6. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ...resources on addgene.org  Antibody GuideRecombinant antibodies available from AddgeneBrowse our recombinant...I love Addgene's new Antibody Guide and not just because I helped create it. But while antibodies, as...space to give proper credit to. Thankfully, as Addgene's resident blog editor, I could take the needed ...alternative reagents are created in the lab, instead of generated in vivo, they have higher reproducibility and ...small enough to be produced in bacteria and are generally cheaper to make than antibodies. Minibodies are...Designed ankyrin repeat proteins, or DARPins, are genetically engineered small affinity proteins, around 14...instead of the antibody constant region. They are generated using combinatorial libraries, and high specificity...

  7. Streaking for Single Colonies: The Streak Plate Challenge

    Type
    Blog Post
    Published
    March 2, 2021, 2:15 p.m.
    ...the blog Resources on Addgene.org Find fluorescent protein plasmids at Addgene Find chromoprotein plasmids...a redo :-( @SandTBioSci #CompetitiveStreaking @Addgene pic.twitter.com/mk44J4Gcj9 — Dave W will get vaccinated...found the chromoprotein plasmids available from Addgene, he knew it would be a perfect fit for the streak...biology. “What I'd like to do with the plasmids from Addgene is have the students transform them into different...transformation experiment, he plans to show how different genetic backgrounds of the host strains affect transformation... Youtube:     Additional resources on the Addgene blog Read our other blog posts on fluorescent ...plasmids at Addgene Check out additional written and video protocols ...

  8. Our New Antibody Mascot is....

    Type
    Blog Post
    Published
    Feb. 24, 2022, 2:15 p.m.
    ...soon with some brand-new Addgene swag!   Last month, we introduced you to Blugene and Aavery's newest friend...friend, our Addgene antibody mascot, here to help us with our upcoming affinity reagent distribution launch...this form or head over to Twitter to vote on @addgene's Twitter poll. Voting is open until Feb. 22, 2022...winning name on Feb. 24. Stay tuned!    Original Blugene and Aavery would like your attention, please, as...that has led them to their biggest discovery yet: Addgene will soon be distributing affinity reagents. Name...hashtag #AntibodyMakesThree on Twitter. As with Blugene and Aavery, we’re looking for a gender neutral ... four will receive some rockin’ newly designed Addgene swag. Good luck and happy naming!   Fig....

  9. New Podcast Segment: Hot Plasmids

    Type
    Blog Post
    Published
    May 17, 2017, 3:37 p.m.
    ...breaking into more audio and video on the Addgene Blog and Addgene website. As we push forward with these...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...present plasmid info with a new segment on the Addgene podcast - The Hot Plasmids Segment. Click on the...Listen to the entire Hot Plasmids episode! We generally publish 5-10 Hot Plasmid articles with each of...Additional Resources on the Addgene Blog Listen to Our Podcast Interview with Eye Researcher Connie Cepko...Science Videos from Tom McFadden Resources at Addgene.org Sign up to Receive Our Newsletter Check Out ...

  10. New Viral Vectors - Spring 2025

    Type
    Blog Post
    Published
    April 29, 2025, 1:30 p.m.
    ... Brian O'Neill.  More resources on the Addgene blog Addgene's Data Hub What is a Virus (and a Viral Vector...Addgene’s viral vector services allow scientists to conduct experiments faster, skipping the preparation...Table 1: New ready-to-use viral vectors in the Addgene repository  Name Serotype Category Depositor...serotype pAAV_hSyn-PdCO-mScarlet-WPRE AAV1 Optogenetics Ofer Yizhar New viral tool pAAV-Ef1a-fDIO...pAAV-Ef1a-fDIO hChR2(H134R)-EYFP AAVrg Optogenetics Karl Deisseroth New viral tool AiP13987: pAAV-AiE0452h_3xC2...different serotypes. Not sure what to request? Addgene's Data Hub can help, with over 100 user-submitted... the link below to keep up with the latest in Addgene's viral vector services and resources!   This blog...

  11. New Viral Vectors - March 2024

    Type
    Blog Post
    Published
    March 5, 2024, 2:15 p.m.
    ...AAV9 Optogenetics Adesnik New viral vector pAAV-nEF-Con/Foff DREADD Gi-mCherry AAV8 Chemogenetics Deisseroth...latest in Addgene's viral vectors services and resources!   More resources on the Addgene blog Addgene's...What's new in Addgene's ready-to-use viral vectors repository? Quite a bit! In this post, we'll share...Table 1: New ready-to-use viral vectors in the Addgene repository  Name Serotype Category Depositor...the full viral services collection or check out Addgene's Data Hub to view viral vector reports from other...

  12. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...fresh columns, regenerated columns had similar DNA yields. Additionally, columns regenerated up to five times...DNA This kit-free plasmid miniprep protocol from Addgene follows a similar workflow as a column-based plasmid...cellulose dipstick. Image: Zou et al., 2018. Regeneration of silica columns for DNA purification Starting... and Deng labs developed a simple method for regenerating silica columns that come with PCR product purification...before a wash with ddH2O and then ethanol. After regeneration, trace amounts of DNA (3 femtograms/ul) could...in the comments! Additional resources on the Addgene blog Read our Plasmids 101 blog series Find other...our plasmid cloning blog posts Resources on Addgene.org Find protocols for molecular biology and cloning...

  13. Great Results Start with Great Standard Curves

    Type
    Blog Post
    Published
    Aug. 15, 2024, 1:15 p.m.
    ...resources on addgene.org Addgene's Antibody Guide Addgene's Ready-to-Use Antibody collection Addgene's Recombinant...create and use a standard curve and provide some general considerations for scientists planning to use them...for the known concentrations (standard curve), generating an equation that allows you to determine the ... triplicate. The closeness of the data points generated by replicate values provides useful information.... With the help of graphing software, you then generate a trendline for the data. The appropriate trendline...R2 never dip below 0.95! More resources on the Addgene blog Antibodies 101: ELISAs The Four ELISAs and...

  14. Networking and Inspiring Young Scientists at Freshers Fairs

    Type
    Blog Post
    Published
    Nov. 17, 2015, 3:30 p.m.
    ...touch! Resources at Addgene Read Our Posts on Networking Get Career Advice from Addgene Bloggers Learn How...and some of the great biological societies that Addgene has had the opportunity to work with at Freshers...the the University of Cambridge and within the general public. The society organises talks by prominent...graduate students, postdocs, faculty members, and the general public. Chemical Society in University College ...develop a range of talks for both students and the general public, career events, and networking meetups. ...outside academia. Inspiring future scientists At Addgene, we’re incredibly glad to see these biological ...society or event which would benefit from some Addgene support? Please get in touch, as we aim to support...

  15. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ...If you’re been following Addgene on #souptwitter or if you just enjoy a warm meal on a winter day, you...molecule into the cell. The fluorescence that is generated allows for qualitative and quantitative measurement...placing the aptamer downstream of a known promoter. Addgene’s Plasmids 101: Aptamer Fluorophores describes the... we’ll take a deeper dive into the content of Addgene’s aptamer soup by exploring the development and ...and applications of fluorescent RNA aptamers in Addgene’s collection. Green fluorescent aptamers Spinach...s group created Spinach2 using site-directed mutagenesis of Spinach. In doing so, they identified the ...changes in intracellular viral rna." Journal of General Virology 98.5 (2017): 992-1003. PubMed PMID: 28555544...

  16. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    Published
    Jan. 22, 2019, 2:41 p.m.
    ... autophagy biosensors currently available from Addgene. Rosella autophagy biosensor Rosella is a dual-...-targeted and mitochondria-targeted Rosella at Addgene! Keima autophagy biosensor Keima is a mammalian...Find the mitochondria-targeted Keima variant at Addgene! mRFP-GFP-LC3 tandem fluorescent protein This autophagy... red puncta. Find the mRFP-GFP-LC3 plasmid at Addgene! GFP-LC3-RFP-LC3∆G: A fluorescent probe that releases...translation, this fusion protein is cleaved to generate one molecule of GFP-tagged LC3 for every one molecule... flux.   Find the GFP-LC3-RFP-LC3∆G plasmid at Addgene! References Klionsky, Daniel J., Ana Maria Cuervo...PMCID: PMC5618514. Additional resources on the Addgene blog Beware of fluorescent protein oligomerization...

  17. Viral Vectors 101: Transductions

    Type
    Blog Post
    Published
    April 30, 2024, 1:15 p.m.
    ...Resources on Addgene.org Generating Stable Cell Lines with Lentivirus Virus Handling and Storage Addgene Viral...Viral Services Resources on the Addgene blog Viral Production at Addgene Viral Vectors 101: What is a Virus...infiltration into a cell. The twist is that the typical genetic cargo — the viral genome — is swapped, at least... viral genome has been swapped for alternative genetic cargo, they can’t reproduce more functional viruses...   Is there an easier way? In fact, there is! Addgene has a number of ready-to-use viral preps available...

  18. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    Published
    May 6, 2014, 1:07 p.m.
    ...his genetically encoded magnesium sensor, the first of its kind. “I hope by depositing at Addgene, people...Institute for Neurobiology has been working on genetically encoded indicators of calcium and other small...Twitch calcium sensors, which are available at Addgene, can be used for ratiometric in vivo imaging. Griesbeck... of the standard organisms for which extensive genetic tools are available. While he recognizes that some...PubMed) Lindenburg et al. MagFRET: The First Genetically Encoded Fluorescent Mg(2+) Sensor. PLoS One. ...(PubMed) Interested in More Hot Plasmids from Addgene? Check out our Hot Plasmid page, where we highlight...some of the popular & newly available plasmids at Addgene. If you're interested in plasmids for in vivo imaging...

  19. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    Published
    Sept. 12, 2019, 1 p.m.
    ... pAAV.hSynap.iGABASnFR (112159-AAV1) Optogenetics Optogenetics continues to be a useful method for manipulating...viral vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols...serotype testing Calcium sensors and GABA sensors Optogenetics Recombinases And more! Control AAV For many...Recombinase AAV Targeting and manipulating specific, genetically-defined neuronal populations with intersectional...pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE (104496-AAVrg) Optogenetics pAAV-hSyn Con/Fon hChR2(H134R)-EYFP (55645-...55645-AAV5)  Additional resources on the Addgene blog Read all of our viral vectors blog posts Find tips...

  20. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    Published
    March 19, 2020, 1:15 p.m.
    ...began working remotely recently? Most of us at Addgene began working from home last week to flatten the...new. We usually have yoga class twice a week at Addgene HQ, but now we’re sharing links to online yoga ...before bed will help you prepare for sleep. At Addgene, our mindfulness group is sticking with their Headspace...virtually connected with the people in your life. At Addgene, we’ve started virtual activities like team lunches...being safe. @GHP_HarvardChan — Shekhar Saxena (@ssaxenageneva) March 16, 2020 Give back to your community...societies hosting virtual networking events. Download Addgene’s Science Career guide to learn more about career...framework to design your future experiments.  Use Addgene’s AAV Data Hub. You can search the AAV Data Hub ...
Showing: 641 - 660 of 930 results