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  1. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...don’t require a PAM sequence at the target locus, making them more flexible than Cas9/Cpf1. Some Cas13 enzymes...

  5. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...started, and 10 more years to obtain FDA approval and make history in the form of Luxturna™. A better vector...

  6. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...domain proteins, they can be expressed in bacteria, making nanobodies a recombinant and renewable anti-IgG...

  7. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ...process – such as the viral DNA polymerase – it makes sense that vectorization strategies include creating...

  8. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...synaptic transmission, are right around 250 nm, making it difficult to study the inner workings of the...

  11. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Oct. 14, 2015, 2:30 p.m.
    ...Find plasmids from this publication at Addgene. Makarova, Kira S., et al. "An updated evolutionary classification...

  12. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...functional before investing time and resources into making a stable cell line. When expressing a viral construct...

  13. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ...gene! STEP 2: Generate an Expression Clone When making the expression clone, it is important to choose...

  16. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ... repaired by cellular mismatch repair machinery making an original C-G pair converted to T-A or reverted...

  18. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ...t simply rely on chance (or random diffusion) to make this happen.  There are several different versions...

  20. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...bbdone with our bbduk process.    At first, to make sure we understood the concept of channels and implemented...
Showing: 641 - 660 of 698 results