We narrowed to 676 results for: Mos;

1. Transferable Skills Guide: External Collaborations

   Type

   Blog Post

   Published

   April 19, 2018, 1:25 p.m.

   ...relationships. Personally, I find this to be the most challenging and time-intensive part of relationship...

2. The PAM Requirement and Expanding CRISPR Beyond SpCas9

   Type

   Blog Post

   Published

   Aug. 20, 2020, 2:30 p.m.

   ...directed repair, since HDR-mediated gene editing is most efficient when target sites are located in close...

3. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

   Type

   Blog Post

   Published

   July 7, 2020, 1:15 p.m.

   ...proteins, finding that APOBEC3A and APOBEC3bctd are the most efficient (Martin et al., 2019). TLDR: The eGFP ...

4. Finding nucleic acids with SHERLOCK and DETECTR

   Type

   Blog Post

   Published

   April 16, 2020, 1 p.m.

   ...type of readout allows for nucleic acid detection almost anywhere as lateral flow strips are easy to transport...

5. Plasmids 101: Plasmid Incompatibility

   Type

   Blog Post

   Published

   March 31, 2020, 1:15 p.m.

   ...same replication control machinery within the cell. Most plasmids encode a negative regulation system involving...

6. An Inside Look into Addgene's Dev Team

   Type

   Blog Post

   Published

   Jan. 23, 2020, 2:45 p.m.

   ... third party libraries and any security updates. Most of the time, updating these libraries is straight...

7. Deciphering the Mysteries of Behavior with Viral Vectors

   Type

   Blog Post

   Published

   Sept. 17, 2019, 12:40 p.m.

   ...studies have been essential for this task. However, most lesioning methods destroy areas larger than intended...

8. Save Time with Transient Plant Leaf Transformations

   Type

   Blog Post

   Published

   July 25, 2019, 12:03 p.m.

   ...tissue (see Figure 3). This method is probably the most scalable method. Transfer your plant into the dark...

9. Transferable Skills Guide: Creativity

   Type

   Blog Post

   Published

   Jan. 18, 2022, 2:15 p.m.

   ... tendency of a group of people to agree with the most prominent idea, and instead building an environment...

10. Antibodies 101: The Four ELISAs and When to Use Them

    Type

    Blog Post

    Published

    July 25, 2024, 1:15 p.m.

    ...source of cross-reactivity.  Sandwich ELISAs In the most common type of ELISA, the sandwich ELISA, users ...

11. Hot Plasmids Spring 2024

    Type

    Blog Post

    Published

    May 23, 2024, 1:15 p.m.

    ...SARS-CoV-2 nucelocapsid protein, one of the virus’s most abundant proteins and a critical component for viral...

12. Plasmids 101: Broad Host Range Plasmids

    Type

    Blog Post

    Published

    April 11, 2024, 1:15 p.m.

    ... plasmids all replicate in E. coli, which is the most commonly used bacteria in lab manipulations. Table...

13. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type

    Blog Post

    Published

    Jan. 24, 2023, 2:15 p.m.

    ...first try to produce GVs in their own lab using the most reliable method, which is the transient cotransfection...

14. Hot Plasmids - November 2023

    Type

    Blog Post

    Published

    Nov. 14, 2023, 3:35 a.m.

    ...undesired edits (indels, etc.), while PE6c and PE6d were most efficient at complex edits, such as long or highly...

15. Viral Production at Addgene

    Type

    Blog Post

    Published

    Oct. 24, 2023, 1:15 p.m.

    ...This article was written by multiple Addgenies and most recently updated by Ina Ersing in August 2023. ...

16. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type

    Blog Post

    Published

    Oct. 13, 2022, 1:45 p.m.

    ...compatible PAM at your site of interest, that is the most efficient option. If there is no NGG in your genomic...

17. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type

    Blog Post

    Published

    Oct. 19, 2017, 1:54 p.m.

    ...high concentration of FLEx vectors guarantees that most cells are transfected with all labeling plasmids...

18. CRISPR Activation: A Practical Guide

    Type

    Blog Post

    Published

    Aug. 18, 2016, 2:30 p.m.

    ...the scientific world by storm. While Cas9 has been most widely used to create specific edits in DNA, there...

19. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type

    Blog Post

    Published

    Aug. 5, 2014, 6:51 p.m.

    ...to press, as there is so much analysis possible. Most scientists are, however, privileged in having exclusive...

20. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type

    Blog Post

    Published

    March 5, 2019, 1:08 p.m.

    ...different area of the cell than CFP and YFP. The most recent derivation of Brainbow, Brainbow 3.0 (Cai...