Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 855 results for: PLE;

Showing: 641 - 660 of 855 results

  1. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    Published
    June 22, 2023, 1:15 p.m.
    ...viruses that can infect people, cells, and animals. Some of these viruses are completely inert, but others ...protection are key to lab safety and the same principles apply to viral handling. Gloves are always required...

  2. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...achieved with a 30-minute incubation, even with multiplexed guides. Figure 1: A) Cell-penetrating ...generated a series of StayGold mutants and identified a triple mutant, Y187F, R144I and T155Q, which they named... Multiple Authors...

  3. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...animal models of disease and cultured cells. For example, in 2013, Jinsong Li and colleagues chose to explore...current delivery technologies are suited for. For example, lipid nanoparticle delivery tends to be trafficked... Multiple Authors...

  4. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    ...have all heard. It’s a truth that extends to both people and the tools we use. Scientists utilize an array... to stop functioning properly or be something completely different than what was originally intended. ...Addgene receives deposited plasmids, they undergo complete plasmid sequencing using an in-house Illumina ...

  5. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    Published
    Jan. 9, 2020, 1:45 p.m.
    ...others are studying and gives you a sense of what people are excited about. Following scientists in fields...more about authors by exploring author pages (for example, Melina Fan’s author page). These pages show all... Key features Setting up a personalized feed is simple. You can enter as many search terms as you like...

  6. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    Published
    Aug. 11, 2016, 2:23 p.m.
    ...anything. Viruses are complicated and they perform complex biological tasks, both of which are factors in ...Ugai et al., 2002). If the virus will be used in a couple of days, some researchers prefer to store freshly...: 16510845. 4. Krajden, Mel, et al. "Effect of multiple freeze-thaw cycles on hepatitis B virus DNA and...

  7. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ...loxP, black arrowheads) and one mutated pair (for example- lox511, white arrowheads). It is necessary to ...express GeneR only in the presence of Cre. In this example, Cre would first invert the reporter and GeneR ...2008. A FLEX switch targets Channelrhodopsin-2 to multiple cell types for imaging and long-range circuit ...

  8. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    Published
    Nov. 19, 2015, 3:30 p.m.
    ...modification is chromatin remodeling. Chromatin is a complex of DNA and histone proteins that compacts cellular...different disease phenotypes, are two fascinating examples of epigenetics in the form of imprinting. In imprinting...have huge impacts in the way we study and treat complex or multifactorial diseases, enabling faster diagnosis...

  9. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    Published
    Sept. 7, 2017, 12:17 p.m.
    ...with knockouts of specific genes. Many years ago, people in the laboratory became frustrated with using ...procedure (illustrated in the YouTube video below) is simple: After obtaining antibiotic-resistant colonies,...initially, or using secondary biological screens. For example, for cancer researchers, if growth properties in...

  10. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type
    Blog Post
    Published
    July 13, 2017, 2:30 p.m.
    ...Compared to humans and mice, flies have a relatively simple genome. They have four chromosomes: the X (and ...chromosomes essentially are messed up chromosomes with multiple inversions. They are unable to participate in ... dominant phenotypes visible on the flies. For example, second chromosome balancers often have the curly...

  11. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    Published
    Dec. 10, 2020, 1 p.m.
    ...AdV-mediated transduction of gRNA:Cas9 ribonucleoprotein complexes into transformed and non-transformed cells yielded...a potential platform that could be realized via simple switching of the relevant gRNAs and donor DNA Adenoviral...vector delivery of RNA-guided CRISPR/Cas9 nuclease complexes induces targeted mutagenesis in a diverse array...

  12. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    Published
    June 25, 2024, 1:15 p.m.
    ...RNA (pegRNA). The pegRNA consists of a target-complementary spacer, a scaffold sequence, and an extension...screens with oligo libraries where pegRNAs are coupled to their target allowing us to edit and evaluate...efficiencies for diverse prime editing systems in multiple cell types. Cell, 186, 1–17. https://doi.org/10.1016...

  13. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    .... For more mechanistic details on HDR and NHEJ, please see the linked blog posts. Compared to its counterparts...strategy looks very similar to HDR, but it is completed using much shorter sequence homologies. Abbreviated...Genome-Editing Based Approach for Mapping Multiprotein Complexes in Human Cells.” Cell Rep. 13: 621-633. PMID: ...

  15. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    Published
    Sept. 6, 2018, 12:02 p.m.
    ...ribonucleoprotein (RNP), consisting of the Cas9 protein in complex with a targeting gRNA, to your cells of interest...CRISPR experiment is the generation of the RNP complex.  While one option is to purchase the Cas9 protein...bread wheat using CRISPR/Cas9 ribonucleoprotein complexes." Nature communications 8 (2017): 14261. PubMed...

  16. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    .... Now GFP and its fluorescent derivatives are a staple in the lab. GFP is used in research across a vast...importance of the entire GFP protein, which is almost completely required to maintain fluorescent activity; very...exist with only slight variations separating them.  Please note that many fluorescent proteins found on the...

  17. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

    Type
    Blog Post
    Published
    July 23, 2019, 12:46 p.m.
    ...of DNA and both identified lymphocyte antigen 6 complex (LY6A) as a receptor for PHP.B. Read on to learn... the majority of astrocytes and neurons across multiple CNS regions. PHP.eB, an enhanced version of PHP.B... interested in using AAV-PHP.B vectors? See Supplementary Table 3 in Huang et al. for a list of PHP.B ...

  18. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ...polycistronic tRNA-gRNA (PTG) gene system to clone multiple protospacers which enables us to target more than...Minkenberg, and Yinong Yang. "Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing...PMC5972399. 12. Molla, K., Shih, J. & Yang, Y. Multiplex CRISPR-mediated base editing in rice. In Genome...

  19. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...granularity, a proxy for nucleic complexity of the cell.  The sample will have been labeled using a panel...parallel.  Let’s imagine we’re looking for cells in a sample that are negative for marker A and positive for...

  20. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    Published
    Oct. 19, 2017, 1:54 p.m.
    ...light inducible ion channels (plasmid # 20298 for example) have revolutionized Neuroscience. They are the...researcher exquisite control over expression of multiple genes in specific cells at specific times. Many.... “A FLEX Switch Targets Channelrhodopsin-2 to Multiple Cell Types for Imaging and Long-Range Circuit ...
Showing: 641 - 660 of 855 results