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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 863 results for: TOR;

Showing: 641 - 660 of 863 results

  1. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    Published
    Aug. 21, 2014, 3:15 p.m.
    .... I had just started month 8 of my 3 year Post-Doctoral contract at the University of Cambridge and knew...outside of academia, a job as a ‘Cancer Marketing Coordinator’ came up at Abcam. Unlike other job descriptions...knew there would be a way around this. First off I tore up my “Scientific CV” and wrote a more business ...

  2. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

    Type
    Blog Post
    Published
    March 4, 2014, 4:49 p.m.
    ...who may not be experts, can you give me a brief history of optogenetics? How long has this idea been around...optogenetic tools for neuroscience back in 2005, both activators as well as silencers of neural activity. Since...brain is very complex and we've hosted hundreds of visitors in our lab to help them understand their specific...

  3. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    Published
    Nov. 27, 2018, 2 p.m.
    ...interactions and growth within the biofilms will then be monitored using fluorescence microscopy and FACS. In an ...to create gene deletions of important unknown regulatory regions in Pseudomonas and Klebsiella spp. The...projects to reach audiences beyond our plasmid depositors and requesters. Check out some of our recent ...

  4. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    Published
    May 16, 2018, 1 p.m.
    ...great deal more information that one might like to store about FPs than is currently represented at fpvis...a centralized, community-editable resource for storage and dissemination of information about FPs. FPbase.org...elaborate spectrum analysis tools, and a better way to store and represent within-study photobleaching comparisons...

  5. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ... plasmids, antibodies, and viral preps in the repository through our hot plasmids articles. Think we missed...expression of the tumor-seeking CAR (chimeric antigen receptor), whereas a ubiquitous promoter drives expression...can be made for expressing your favorite T-cell receptor, or perhaps any other tool that can benefit from...

  6. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    Published
    Aug. 2, 2022, 1:15 p.m.
    ...classification system, including its history, biology, and usages in the lab. History In 1884, while studying infected...The classification system is also useful in a laboratory setting. When designing a plasmid to be inserted...

  7. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    Published
    June 23, 2022, 1:15 p.m.
    ...correct the cystic fibrosis transmembrane conductor receptor (CFTR) by homologous recombination in cultured...control the CRISPR system activity.  Using an AAV vector may help with size limitations. In addition, non-viral...

  8. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    Published
    March 24, 2016, 2:30 p.m.
    ...contributed by guest blogger Chris Richardson, a Postdoctoral Researcher in Jacob Corn’s lab. CRISPR-Cas9 ...which causes the breaks) and host cell DNA repair factors (which repair those breaks) makes Cas9 extremely... optional donor DNA. Chris Richardson is a Postdoctoral Researcher in Jacob Corn’s lab investigating...

  9. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    Published
    Dec. 18, 2014, 4:06 p.m.
    ...how I got my current job as a Communications Coordinator. Wondering how you can develop more transferable...Theresa Liao (@theresaliao) is the Communications Coordinator for the Department of Physics & Astronomy, University...Experimental Medicine. She is the Community Relations Director for Curiosity Collider (hyperlink https://curiositycollider.org...

  10. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    Published
    July 19, 2018, 1:12 p.m.
    ...Excellent scientists learn from advisers and other mentors by collaborating as they craft sentences for abstracts...in all STEM fields become excellent science communicators. To empower graduate students to become ambassadors...careers in science communication The strength of story telling Science communication: 9 strategies to get...

  11. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    Published
    Nov. 21, 2019, 1:53 p.m.
    ...team and software engineers to update our Lab Inventory Management System (LIMS) to accommodate our new... discussed them in meetings and 2) creating a pictorial guide for our engineers (Fig. 2). Creating this... a lot on context. New people in the lab or collaborators will not have the context you do, which means...

  12. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    Published
    Sept. 24, 2019, 12:55 p.m.
    ...is correct. It's also best to use 1-2 ug of the vector for digestion. PCR Design primers that overlap ... probably left your plate for too long in the incubator. The small colonies are called satellite colonies...after the screening, sequence the plasmid. Many vectors contain sequences that are complementary to standard...

  13. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    Published
    Aug. 16, 2018, 12:40 p.m.
    ...one to study RNAs expressed from their native regulatory elements. Controls for RaPID experiments RaPID...Ramanathan et al. also used the Contamination Repository for Affinity Purification (CRAPome), to identify...RNA-binding protein that’s highly expressed in neural progenitor cells. Knock-down of QKI decreased Zika viral...

  14. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    Published
    Jan. 16, 2018, 3:20 p.m.
    ...inspiration to resolve the technical hurtle of monitoring protein turnover of specific proteins in situ...Erin M Schuman, a former Caltech researcher, now Director at the Max Planck Institute for Brain Research...to all audiences. Right now, she is a science instructor at The Innovation Institute in Boston. When Eugenia...

  15. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    Published
    April 20, 2023, 4 p.m.
    ...interview better! As a scientist turned science communicator, I’ve done a lot of public speaking, and I'm...six figures. Averaging ten panels each. (For this story, it is important to know that the student presenting... turn your slide deck into a chalk talk. Your translator doesn’t know the term “diving board” so you end...

  16. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    Published
    June 8, 2017, 2:30 p.m.
    ...of emitted photons before they are destroyed. Factors that affect photostability The photobleaching rate...fluorophore will bleach. This is, however, not the full story. The photobleaching rate does not depend on excitation... for each of these conditions. On top of these factors, environmental conditions such as cellular redox...

  17. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ...way they are, the research antibodies in your refrigerator often need a little help to be useful. Mainly...of e-Amino Groups Available for Conjugation of Effector Molecules to Monoclonal Antibodies. Hybridoma ...resources on the Addgene blog Antibodies 101: Buffers, Storage, and ConjugatesAntibodies 101: The Basics of Western...

  18. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    Published
    Nov. 24, 2015, 3:30 p.m.
    ...Addgene, we love GFP, and we’re always excited when depositors find new ways to make this workhorse protein ...GBPs together to create a complete transcription factor, activating luciferase transcription. Tang et al...delivered throughout the nervous system, including the motor cortex, cerebellum, and spinal cord. Cre-DOG is ...

  19. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    Published
    Feb. 11, 2014, 12:13 p.m.
    ...aligns with your work, your advisor may want to mentor you during the grant application process. When ...first. Additionally, reach out to peers and collaborators who have succeeded at grant writing to see what...write your first grant proposal" by Soapbox Science Editor in Nature Blogs "How to write a research project...

  20. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    Published
    Nov. 14, 2024, 2:15 p.m.
    ...They play important roles in many cellular and regulatory processes, but much of their functionality is...effects can be minimized by disrupting lncRNA regulatory elements that are crucial for transcription and..., on the other hand, recruits transcriptional activators such as VP64, SAM, or SunTag to specific ncRNA...
Showing: 641 - 660 of 863 results