We narrowed to 719 results for: Tat

1. Building and Validating Recombinant Antibodies: A Case Study

   Type

   Blog Post

   Published

   April 5, 2022, 1:20 p.m.

   ...). Figure from Andrews, et. al., Elife  A limitation of hybridoma generated mAbs is that if the frozen...

2. Antibodies 101: Isotypes

   Type

   Blog Post

   Published

   Oct. 19, 2021, 1:15 p.m.

   ...that helps attack extracellular pathogens.  Transportation of antibodies to places they can’t reach on...

3. GCE4All: Making Genetic Code Expansion Accessible

   Type

   Blog Post

   Published

   June 15, 2023, 1:15 p.m.

   ...GCE4All, an NIH-funded center located at Oregon State University focused on turning GCE tools developed...

4. Viral Vectors 101: Viral Applications

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   Blog Post

   Published

   April 13, 2023, 1:15 p.m.

   ... have been engineered to introduce frameshift mutations in genes of interest, turning expression off permanently...

5. Viral Vectors 101: Gamma-Retroviral Packaging Systems

   Type

   Blog Post

   Published

   Aug. 6, 2024, 1:15 p.m.

   ...by long terminal repeat (LTR) sequences, which facilitate integration of the transfer plasmid sequences...

6. GPCRs: How Do They Work and How Do We Study Them?

   Type

   Blog Post

   Published

   Feb. 11, 2021, 2:15 p.m.

   ...create chemogenetics receptors, GPCRs were first mutated via site-directed mutagenesis to bind non natural...

7. Developing a cold tolerant E. coli using specialized chaperones

   Type

   Blog Post

   Published

   Jan. 28, 2020, 2:35 p.m.

   ... means recombinant proteins can aggregate or precipitate out as insoluble granules. We overcame this problem...

8. Behind the scenes: Addgene’s new search engine and more

   Type

   Blog Post

   Published

   Dec. 12, 2019, 2:22 p.m.

   ...engine unique. Most importantly, with this new implementation, we laid the foundations for many improvements...

9. How to Make Friends and Meet People at a Scientific Conference

   Type

   Blog Post

   Published

   Aug. 7, 2014, 1:58 p.m.

   ...exhausted that you can’t get a lot from the presentations and programs. After the conference Follow ...

10. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type

    Blog Post

    Published

    July 10, 2014, 7:17 p.m.

    ... to achieve success in this business. A good presentation can help win a business plan competition and...

11. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

    Type

    Blog Post

    Published

    April 23, 2018, 2 p.m.

    ...modeling. Its reversibility allows precise and quantitative analysis of epigenetic memory at nearly any ...

12. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type

    Blog Post

    Published

    March 3, 2020, 2:15 p.m.

    ...further tested the base editors by correcting a mutation that causes Niemann-Pick disease type C, directly...

13. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type

    Blog Post

    Published

    Jan. 31, 2019, 1:32 p.m.

    ...let’s take a look at exosomes. Proficient in transportation, exosomes are tools of communication and provide...

14. CRISPRainbow and Genome Visualization

    Type

    Blog Post

    Published

    Feb. 28, 2017, 3:30 p.m.

    ...fluorescently labeled Cas9’s come with a number of limitations. Since color is specified by PAM sequence, each...

15. Giving gRNAs a Facelift - Synthetic and Beyond

    Type

    Blog Post

    Published

    Oct. 20, 2022, 1:15 p.m.

    ...as the unnecessary stress of worrying about the status of your RNA. gRNAs modifications are even going...

16. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type

    Blog Post

    Published

    Aug. 5, 2021, 12:15 p.m.

    ...painful stimuli are processed by a network of excitatory and inhibitory neurons whose function and connectivity...

17. Antibodies 101: Polyclonal Antibodies

    Type

    Blog Post

    Published

    July 14, 2021, 1:15 p.m.

    ...protein in low abundance or whose conformational state may change. If your protein might become slightly...

18. Antibodies 101: Conjugation

    Type

    Blog Post

    Published

    March 26, 2024, 1:15 p.m.

    ...Adler M, Wacker R (2007) Detecting antigens by quantitative immuno-PCR. Nat Protoc 2:1918–1930. https://...

19. To Each HIS Own

    Type

    Blog Post

    Published

    March 2, 2023, 2:15 p.m.

    ... H, Gentz R and Stüber D. Genetic Approach to Facilitate Purification of Recombinant Proteins with a Novel...

20. Plasmids 101: The protein expression toolbox

    Type

    Blog Post

    Published

    Feb. 16, 2023, 7:05 p.m.

    ... gRNA is all you need to generate a frameshift mutation that will permanently ablate protein expression...