We narrowed to 806 results for: Tes
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TypeBlog PostPublishedMay 8, 2018, 1 p.m....checked circle. Typically, a yellow check mark indicates that a particular parameter was automatically ...sequencing bases downstream of the cut. Summary ICE generates NGS-quality CRISPR editing analysis from Sanger...
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Tips for Writing a Good Cover Letter
TypeBlog PostPublishedMay 21, 2024, 1:30 p.m....for labs and nonprofits, who tend to prioritize candidates that are excited about their work and mission... will be close to that of a professional email. Notes from real-life hiring managers In preparation for... -
Viral Vectors 101: Pseudotyping
TypeBlog PostPublishedApril 20, 2021, 2:30 p.m....transduction of primary lymphocytes and CD34+ cells derived from human and nonhuman primates. Blood 100:823–832... -
AAV Titers: Where Do They Come From And What Do They Mean?
TypeBlog PostPublishedNov. 15, 2017, 3 p.m....particles (black arrowhead). Scale bar = 100nm. Image courtesy of David Bell and Svetla Stoilova-McPhie, Center...assay. This may be due to competition for binding sites between the primers and the AAV genome repeats. ... -
Transferable Skills Guide: Career Planning Resources
TypeBlog PostPublishedNov. 14, 2017, 2 p.m....of recruiters for identifying appropriate job candidates. If you do receive such a call, make sure to ...-out-of-job-fairs-2014-3 https://www.forbes.com/sites/neilpatel/2015/05/21/15-surprising-rules-you-should-know-regarding-linkedin-etiquette... -
Healthcare Consulting: A Door to the Business of Life Sciences
TypeBlog PostPublishedOct. 13, 2016, 2:30 p.m....preclinical and clinical research to discover and test the best new therapeutics. Consultants often work...PhDs, and an increasing recognition of the value graduates bring to these roles. Healthcare consulting is... -
Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs
TypeBlog PostPublishedOct. 27, 2015, 3 p.m.... Jennifer Lippincott-Schwartz at the National Institutes of Health and he is currently an Associate Professor...Paladino, Simona, et al. "Protein oligomerization modulates raft partitioning and apical sorting of GPI-anchored... -
Data Freedom: The Expansion of Data Sharing in Research Publications
TypeBlog PostPublishedAug. 5, 2014, 6:51 p.m....others understand ours. Improving access to data promotes dissemination, especially for young researchers...useful to others and efforts to enhance these attributes should be welcomed. However, expectations must... -
Harnessing Bacterial Toxins for Allelic Exchange
TypeBlog PostPublishedAug. 15, 2019, 12:30 p.m....of toxins before the second crossover step, they tested their expression under both rhamnose- and arabinose-inducible...selective RNA unfolding and sliding at upstream standby sites." Nucleic acids research42.4 (2013): 2646-2659. ... -
Five Popular Model Organisms
TypeBlog PostPublishedApril 11, 2019, 12:39 p.m....misfolding that leads to a build-up of protein aggregates termed “lewy bodies” in the brain. Yeast models...majority of C. elegans are also self-fertile hermaphrodites making it easy to conduct large experiments... -
Quick Guide to Near-Infrared Fluorescent Proteins
TypeBlog PostPublishedOct. 5, 2021, 1:15 p.m....neurons in the neocortex of a living mouse. Image courtesy of Axel Nimmerjahn (Salk Institute for Biological...tags due to the proximity of the N- and C-termini. Latest miRFPnanos9 do not yield to (m)iRFPs in cellular... -
Antibodies 101: Normalization and Loading Controls for Western Blots
TypeBlog PostPublishedApril 8, 2025, 1:15 p.m....total protein are compared. While a higher slope indicates higher sensitivity, linearity is the most important... than the total protein quantification. This illustrates why total protein measurement is often more accurate... -
CRISPR 101: Validating Your Genome Edit
TypeBlog PostPublishedNov. 3, 2022, 12:15 p.m....doesn’t affect any endogenous restriction enzyme sites? Never fear! You can introduce a silent, passenger...CRISPRitz or CRISPOR can help narrow down the list of sites where off target editing may occur, allowing you... -
Using Phosphoserine to Study Protein Phosphorylation
TypeBlog PostPublishedJune 23, 2016, 2:30 p.m.... for the majority of documented phosphorylation sites. If knowledge of a candidate regulatory kinase is...that binds specifically to phosphate moieties and promotes a robust mobility shift in phosphorylated, but... -
Thoughts on the Future of Research 2015 Boston Symposium
TypeBlog PostPublishedNov. 25, 2015, 4 p.m....why they are paid relatively low wages (Stephan estimates that postdocs are paid $16.15/h before fringe ...and Graduate Student Career Outcomes University websites do not currently publish career outcomes for PhDs... -
Antibodies 101: Multiplex Immunofluorescence
TypeBlog PostPublishedMay 23, 2023, 1:15 p.m....secondary antibody only labels sites where you expect the target to be. You can test this by preparing a sample... -
Live and Let Dye: Self-Labeling Protein Tags
TypeBlog PostPublishedApril 16, 2024, 1:15 p.m....). In vivo protein labeling with trimethoprim conjugates: A flexible chemical tag. Nature Methods, 2(4..., G., Tünnermann, J., Tänzer, T., Karpenko, J., Mertes, N., Xue, L., Uhrig, U., Reinstein, J., Hiblot,... -
New Antibody Animation!
TypeBlog PostPublishedFeb. 22, 2024, 3:13 p.m....Ready to learn about antibodies? Our latest animation is here to help! Join Abi as they explain what ... -
New Coomassie Protocol Video
TypeBlog PostPublishedOct. 31, 2023, 1:15 p.m....determine your antibody's purity? Check out our latest protocol video, "Coomassie Purity Stain of Recombinant... -
New Antibodies in the Lab Animation!
TypeBlog PostPublishedNov. 19, 2024, 2:15 p.m....where to start or what your options are? Addgene's latest animation is here to help! "Antibodies 101: Antibodies...