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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 886 results for: URE

Showing: 641 - 660 of 886 results

  1. What's New in CRISPR - September 2019

    Type
    Blog Post
    Published
    Sept. 10, 2019, 12:56 p.m.
    ...active in both E. coli and human cells. Using the structure of CasX at eight different states of assembly .... coli and human expression. Read the paper in Nature Find the CasX plasmids Drug inducible transcriptional...sequence-specific toxicity of Cas9. Read the paper in Nature Communications Find the Bikard E. coli Genome-wide...

  2. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    Published
    July 9, 2015, 2:30 p.m.
    ...scientist in training can adopt to ensure opportunities in the future. Studies show that the majority of...an excellent resume ready to be submitted The structure and content of a resume can be crucial for a successful...

  3. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

    Type
    Blog Post
    Published
    July 29, 2014, 6:16 p.m.
    ...proteins known to localize to specific cellular structures.The Davidson lab even included some of their own... see Supplemental Documents here). If you’re not sure where your protein of interest localizes in the ...easier for scientists everywhere to get a clearer picture of that intracellular world, even as the Davidson...

  4. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    Published
    April 1, 2014, 5:33 p.m.
    ...This blog post is the first in a series that will feature advice for students, postdocs, and young Principal...their top 3 tips. Tom Ellis 1. Keep up with the literature. He recommends setting up a few Pubmed email ...“If you don't read, you're doomed.” 2. Always be sure to include various positive and negative controls...

  5. In Living Color: The Skinny on In Vivo Imaging Tools

    Type
    Blog Post
    Published
    March 27, 2014, 5:14 p.m.
    ...paper that appeared in Nature Biotechnology back in 2011 and then in another Nature Methods paper last year... far-red light. As the researchers described in Nature Communications last year, “The capability to control...

  6. Build Your CRISPR Vocabulary

    Type
    Blog Post
    Published
    July 2, 2024, 1:15 p.m.
    ...proteins, DNA, and RNA used in CRISPR.   Figure 1: Overview of the parts of CRISPR. The bacterial...in the CRISPR array that will become guides for future targeted degradation. Before integration into the...CRISPR–Cas immunity and genome editing enzymes. Nature Reviews Microbiology, 20(11), 641–656. https://...

  7. CRISPR 101: Drug discovery and CRISPR

    Type
    Blog Post
    Published
    May 4, 2023, 1 p.m.
    ...and a drug is developed, it’s good practice to measure the specificity of that target and validate the... is where CRISPR can save time and money to make sure quality candidates are selected.      CRISPR...discovery pipeline.   CRISPR and drugs in the future While CRISPR has already done a lot for drug discovery...

  9. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    Published
    April 2, 2020, 6:17 p.m.
    ...archaea and eukaryotes such as yeast and plants. In nature, plasmids provide one or more functional benefits...for the life scientist or bioengineer.   Figure 1: Map of a plasmid with its elements described...constructed in the lab? Due to their artificial nature, lab plasmids are commonly referred to as “vectors...

  10. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type
    Blog Post
    Published
    June 19, 2018, 1:38 p.m.
    ...of soundwave pressure required to collapse them: arg2 vesicles collapse at a lower pressure than arg1 vesicles...noninvasive imaging of microorganisms in mammalian hosts. Nature, 553, 86-90. PubMed PMID: 29300010. Additional ...

  11. Plasmids 101: Positive and Negative Selection for Plasmid Cloning

    Type
    Blog Post
    Published
    Aug. 22, 2019, 12:43 p.m.
    ...get it into your plasmid backbone, and zap the mixture of DNA into cells. Unfortunately, not every cell...post, but don’t worry, we’ll cover screens in a future post. Positive selection: Cells that have gained...toxin genes, and the sacB gene). By tapping into nature’s toolbox, scientists are finding more and more ...

  12. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

    Type
    Blog Post
    Published
    July 16, 2019, 12:59 p.m.
    ... distinct C-bend to escape possible danger (see figure). Larvae also perform spontaneous slow swimming...distinguish between swimming behaviors, the researchers measured the tail movements of 5 day old fish. The larvae...behavioural module in the vertebrate spinal cord." Nature 461.7262 (2009): 407. PubMed PMID: 19759620. PubMed...

  13. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    Published
    Aug. 19, 2014, 3:39 p.m.
    ...in our repository and will discuss some of the features you may want to consider as you make your choice...vector that you will need. You will also have to make sure that your plasmid has been incorporated into the...promoter. Other vectors can be expressed in fly culture cells like S2 and carry the metallothionein promoter...

  14. Advice for Choosing a Research Project

    Type
    Blog Post
    Published
    April 17, 2014, 2:45 p.m.
    ...advice from the successful PIs who were previously featured in "9 Tips to Achieve Success in Academia". Important...evaluated your plan? Have you read the relevant literature to understand where the field is today? Have ...the importance of keeping up-to-date with the literature of a couple of well-known journals that discuss...

  15. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    Published
    April 15, 2014, 3:11 p.m.
    ...indefinitely at room temperature (thus also saving freezer space).  The procedure becomes less attractive... agar slices.  Molecular biologists used this procedure daily before the advent of techniques like PCR...

  16. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    Published
    Jan. 30, 2014, 3:29 p.m.
    ...selective media, and provides those bacteria with a pressure to keep your plasmid. Viva la (bacterial) resistance... antibiotics against Gram negative bacteria. In future posts, we'll detail selection in non-bacterial ...strains have natural antibiotic resistances, so make sure your plasmid and E. coli strain are compatible! ...

  17. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    Published
    July 21, 2022, 1:15 p.m.
    ...the public in a meaningful way, and not solely to ensure a profit. Often, these companies believe they possess...chance to carry out a comprehensive review of the literature informing the notion of “heading” in soccer as... of my thesis: a survey instrument designed to measure how individuals respond to issues of unsettled ...

  18. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    Published
    June 9, 2022, 1:15 p.m.
    ...the foundation of all good communication is making sure everyone’s on the same page. So for our first post...education is education that is provided through a structured environment such as the classroom or a defined...specific audience - giving consistency, purpose, structure, and intent to the messaging - while also creating...

  19. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    Published
    March 28, 2022, 4:30 p.m.
    ...colleagues and is invested in improving research infrastructure, from our educational materials to our repository...and ScFvs, based on community demand. Figure 1: Antibody against PSD-95 from the Trimmer Lab...Reproducibility: Standardize antibodies used in research. Nature 518:27–29. https://doi.org/10.1038/518027a  Resources...

  20. RUBY-Red Siliques

    Type
    Blog Post
    Published
    March 1, 2022, 2:15 p.m.
    ...seedlings or callus (undifferentiated plant cell culture), which is great for a wide variety of applications...found in all other plants, anthocyanins. Figure 1: An adult plant expressing RUBY. Photos by Andrew...can be selected without antibiotics. (Top left) Mature silique (Arabidopsis fruit) with fertilized developing...
Showing: 641 - 660 of 886 results