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We narrowed to 919 results for: addgene

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Showing: 641 - 660 of 919 results

  1. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    Published
    Aug. 10, 2021, 1:15 p.m.
    ...concentration of the original template DNA in the sample.  Addgene currently uses ddPCR for AAV titrating. You can...Elsevier, pp 215–247 Additional resources on the Addgene blog Droplet Digital PCR for AAV Quantification...Site Directed Mutagenesis by PCR Resources on Addgene.org Protocol: Polymerase Chain Reaction (PCR) Protocol...

  2. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type
    Blog Post
    Published
    June 22, 2017, 2:30 p.m.
    ...constructs you create in your own work available to the Addgene community. Download our Markers of Subcellular ... FRET Microscopy. Additional Resources on the Addgene Blog Learn More about Plasmids for Endogenous ...Collection Get Tips for Using FRET Resources on Addgene.org Browse the Allen Institute for Cell Science ...

  3. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    Published
    Jan. 10, 2017, 3:37 p.m.
    ...    Alejandro (Alex) Chavez is a member of the Addgene Scientific Advisory Board. His laboratory at Columbia...PMCID: PMC4265475. Additional Resources on the Addgene Blog Listen to Our Interview with Alex Chavez ...: A Practical Guide Additional Resources on Addgene.org Check Out Our CRISPR Guide Pages Find Empty ...

  4. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    Published
    March 28, 2018, 6:52 p.m.
    ...this paper are available at Addgene.  Additional Resources on the Addgene Blog Learn about Engineered...about Homology Directed Repair Resources on Addgene.org Check out our CRISPR Guide Find CRISPR Plasmids...

  6. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    Published
    April 4, 2019, 12:53 p.m.
    ...bacteria of interest? Find Mobile-CRISPRi plasmids at Addgene! References Auchtung, Jennifer M., et al. "Biology...PubMed PMID: 26104363. Additional resources on the Addgene blog Learn about other CRISPR methods for bacterial...libraries Read all CRISPR blog posts Resources on Addgene.org Browse our CRISPR interference plasmids Find...

  7. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type
    Blog Post
    Published
    Feb. 7, 2019, 2:16 p.m.
    ...at existing tools. As has been written here on Addgene, there has been a Cambrian-like explosion of software... Clement Andy May Additional resources on the Addgene blog Read our CRISPR 101 blog posts Learn about...gRNAs Find other CRISPR software Resources on Addgene.org Find CRISPR plasmids and resources Download ...

  8. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...Antibody Resources on Addgene.org  Find recombinant anti-c-Myc [9E10] at Addgene Find plasmids with the...scisignal.aax9730  Additional resources on the Addgene blog Antibodies 101: Monoclonal Antibodies Plasmids...

  9. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    Published
    Aug. 11, 2016, 2:23 p.m.
    ...standardize your experiments later. Stay tuned to the Addgene blog and be sure to check out our viral vector ... PMCID: PMC330758. Additional Resources on the Addgene Blog Learn All about Adenovirus Associated Virus...Lentiviral Vectors Additional Resources on the Addgene.org Check out Our Viral Vector Guide Pages Find ...

  10. Plasmids 101: Colony PCR

    Type
    Blog Post
    Published
    May 12, 2016, 2:30 p.m.
    ...back-bone specific primers. Find Empty Backbones at Addgene Each approach has advantages and drawbacks, which...Overview of Colony PCR Additional Resources on the Addgene Blog Learn to do Restriction Cloning Try SLIC ...about Gibson Assembly Additional Resources on Addgene.org Read about Choosing a Molecular Cloning Technique...

  11. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...More examples of these biosensors can be found at Addgene. Split FPs – some FPs (e.g. GFP, Venus) can be...More Helpful Websites & Resources: FP guide at Addgene Interactive Visualization of Fluorescent Protein...

  12. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ...lentiviral-based CRISPR libraries available at Addgene. The large packaging capacity and stable expression...s41392-024-01780-w Additional resources on the Addgene Blog Overcoming the AAV Size Limitation for CRISPR...Using the FITS Approach Additional resources at addgene.org CRISPR pooled libraries Guide to pooled libraries...

  13. Design Tips for Prime Editing

    Type
    Blog Post
    Published
    Jan. 23, 2025, 2:15 p.m.
    ...programs to help you design pegRNAs. Here on the Addgene blog, we featured a guest blog by the developer...s41586-024-07259-6 Additional Resources on the Addgene blog Prime Editing: Adding Precision and Flexibility... Download the CRISPR 101 eBook Resources on Addgene.org Read the CRISPR guide Find CRISPR plasmids by...

  14. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    ...Your Lentivirus Preps More resources on Addgene.org Addgene's virus protocols Viral Plasmids and Resources...Resources and references More resources on the Addgene blog Viral Vectors 101: Producing Your rAAV AAV...

  15. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    Published
    July 25, 2024, 1:15 p.m.
    ...Polyclonal Antibodies Resources on addgene.org The Addgene Antibody GuideAddgene's recombinant antibody collection...luck and happy binding! More resources on the Addgene blog Antibodies 101: ELISAsAntibodies 101: Selecting...

  16. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...43R] has been one of the hottest antibodies in Addgene’s collection since we began distribution and has...[K28/43R] under the Application section of the Addgene antibody page, and learn more about the whole-brain...

  17. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    Published
    Oct. 10, 2023, 1 p.m.
    ...doi.org/10.1093/mutage/gei016I Resources on Addgene.org Addgene’s Basic Molecular Biology Protocols Over-...Antibiotic Plating Plasmids 101 eBook Resources on the Addgene blog Plasmids 101: Antibiotic Resistance Plasmids...

  18. How to Be an Excellent Trainee

    Type
    Blog Post
    Published
    Sept. 26, 2023, 1:15 p.m.
    ...biology you will study. (You can also check out Addgene’s blog, eBooks, and other educational resources ...personnel! Happy training! Resources Resources on the Addgene blog Trading Spaces – Visiting Scientist Edition...

  19. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ...Vectors 101: Viral Applications Resources on addgene.org Addgene's Lentiviral Guide Most popular lentiviral...Resources and References More resources on the Addgene blog Viral Vectors 101: Viral Vector Elements ... This blog was written by Addgenie Brian O'Neill, with thanks to Addgenie Rebecca Hutcheson for her assistance...
Showing: 641 - 660 of 919 results