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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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In Category

We narrowed to 833 results for: ins

Showing: 641 - 660 of 833 results

  1. Top Requested Plasmid of 2017 - pMD2.G

    Type
    Blog Post
    Published
    Jan. 10, 2018, 4:13 p.m.
    ...CMV promoter. Envelope proteins are a key component of lentiviruses. These proteins coat the virus and help...

  3. CRISPR Cheat Sheet

    Type
    Blog Post
    Published
    Jan. 27, 2021, 2 p.m.
    ...to cut other DNA or RNA sequences. Nucleases: Proteins that bind to gRNAs and are directed by the gRNAs...prime editing and the identification of other Cas proteins continue to expand the targeting range. CRISPR...

  4. Countdown to Halloween @Addgene

    Type
    Blog Post
    Published
    Oct. 29, 2014, 5:26 p.m.
    ...tradition in which we choose our teams and start brainstorming months in advance. Our teams comb the news (...Friday. So if you’re interested in finding out who wins this year’s Addgene costume contest, like us on ...

  5. Editor's Choice, October 2016

    Type
    Blog Post
    Published
    Nov. 4, 2016, 2:30 p.m.
    ...academic institutions can afford to hire. It will be difficult for some of these institutions to manage...

  6. Editor's Choice, July 2016

    Type
    Blog Post
    Published
    Aug. 5, 2016, 3 p.m.
    ... about how fluorophores other than fluorescent proteins can be incredibly useful in FRET experiments. ... our posts if you have any questions or useful insights. If you're interested in blogging for us, fill...

  7. Newly Updated AAV Data Hub!

    Type
    Blog Post
    Published
    Feb. 7, 2023, 2:15 p.m.
    ... share their AAV validation data. The Data Hub contains a curated collection of community submissions ...) can save you significant time and money. For instance, this report shows that anterograde, monosynaptic...

  8. Podcast: Interview with Addgene's Founders

    Type
    Blog Post
    Published
    April 13, 2018, 12:51 p.m.
    ...and metabolism. Melina has worked at Genetics Institute in Cambridge, MA and Sequana Therapeutics in San...the Addgene Blog Addgene's a nonprofit? Get an Inside Look at Addgene's NGS Quality Control Addgene's...

  9. Grad School Advice Part 2: Building Community

    Type
    Blog Post
    Published
    Oct. 6, 2016, 2:30 p.m.
    ...Sciences program at Harvard University and pick their brains on how they've managed to keep themselves happy...year dip (see graph). Niroshi and Ben give their insights as to what causes this dip. 11:11 - 15:37 : How...

  10. The Addgene Antibody Data Hub

    Type
    Blog Post
    Published
    Sept. 12, 2024, 1:15 p.m.
    ...submission is used to create a data report, which contains experimental data showing how the antibody performed...immunocytochemistry, ELISA — but in all cases, it contains a detailed description of the experimental conditions...

  11. Bringing Sustainable Practices to the Lab: Easy Action Items

    Type
    Blog Post
    Published
    April 22, 2019, 12:26 p.m.
    ...probably bring in a mug or reusable water bottle instead of buying yourself a big stack of plastic cups....could be: Setting up a recycling program at your institute. Set your -80 °C freezer to -70 °C and teach other...

  12. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    Published
    Jan. 11, 2017, 8:47 p.m.
    ... technologies were without controversy, and it remains to be seen how popular any one of them will become... though a little confusing to the uninitiated, contains much of the information needed to understand how...

  13. Science Communication for Everyone

    Type
    Blog Post
    Published
    April 16, 2019, 1:05 p.m.
    ...sessions led by scientists, which is hosted on our Instagram channel every Sunday. What's planned for the upcoming...participate in our one-hour Live Q&A session on Instagram every Sunday. Undergraduate and graduate students...

  15. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    Published
    Aug. 28, 2018, 12:04 p.m.
    ...loads of inspiration for the current and the next generation of microbiologists. If you got inspired to produce...

  16. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    Published
    Jan. 28, 2014, 3:10 p.m.
    ...protocol has worked well for us for multiple E. coli strains, including DH5alpha, XL1 Blue, TOP10, ccdB survivalTM...competent cells, then you're best off following the instructions that came with them. If you decided to make ...

  17. Let There Be LITE Plasmids

    Type
    Blog Post
    Published
    Nov. 21, 2013, 3:56 p.m.
    ...lab at MIT, the use of customizable DNA-binding domains based on transcription-activator-like effectors...control gene expression in mouse neurons and in the brains of living mice both quickly and significantly. ...

  19. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    Published
    March 22, 2016, 2:30 p.m.
    ...Kathleen Ottina and David Sabatini at the Whitehead Institute and MIT, started with a list of 17 signaling pathways...pathways. With support from the National Cancer Institute, they expect to add another 200 or 250 constructs...

  20. What's New in CRISPR - December 2019

    Type
    Blog Post
    Published
    Dec. 17, 2019, 2:37 p.m.
    ...bionanoparticle system for packaging Cas9/gRNA ribonucleoproteins for transient expression. They replaced the...ADAR2 into a cytidine deaminase. This editor still retains A-to-I editing. This system is called RNA Editing...
Showing: 641 - 660 of 833 results