We narrowed to 846 results for: ins

... break, or is engineered not to cleave DNA and instead activates/represses gene expression by various ...minimum of 1,000 targeted cells to sequence. In this instance, a 5% off-target frequency is something to keep...-defective lentiviral vectors - which rely on insertion of DNA by the NHEJ pathway during DNA repair of...

...targets, you might decide to do simultaneous IF instead (Figure 1B). To label all your targets in one step...yourself. If you can’t find a suitable antibody against your target protein, consider fusing a protein ...Neumann, E., De Caestecker, M., Gutierrez, D., & Spraggins, J. (2022). Multiplex Immunofluorescence on Fresh...

... a summer role as a Research Scientist at the Institute of Cancer Research in Sutton, located on the edge...and plenty of jobs in science at world renowned institutes. The lab at the University of Queensland required...field don’t really get to do a lot of lab work but instead really need to be comfortable with programming....

...transdifferentiation of glucagon-producing α-cells into insulin-producing β-like-cells [37, 38]. In 1987, Davis... ES cells and iPS cells into mature pancreatic insulin-producing cells. Cell Res, 2009. 19(4): p. 429-...duodenal homeobox gene 1 induces expression of insulin genes in liver and ameliorates streptozotocin-induced...

...used GAPDH has noted issues as a reference gene (Higgins, 2000; Ref, Zhu, 2002), though there are cases ...run and the difference in CT values is plotted against the log input concentration for each dilution. ...://doi.org/10.1038/s41598-018-26707-8Suzuki T; Higgins PJ; Crawford DR. Control selection for RNA quantitation...

...a template. To do so, they used a Cas nickase, instead of dCas9. The resulting editor, BE3, nicks the ...and/or evolved to catalyze cytidine deamination instead of (or in addition to) adenosine deamination (Chen... R. T., Christie, K. A., Whittaker, M. N., & Kleinstiver, B. P. (2020). Unconstrained genome targeting...

...Bluesky: @addgene.bsky.social; or @addgene on Instagram, TikTok, Facebook, and LinkedIn. We are also exploring...and features that previously made the platform a mainstay of conversation in the scientific community. Addgene...

...of our holiday decorations, our holiday spirit remains. We would like to thank everyone who participated...creating memories with their labmates! Are you inspired by our 2017 #Deckthelab winners? Be sure to enter...

...NanoMEDIC particles Akitsu Hotta pHCMV-RabiesG Contains Rabies virus G protein; see depositing paper for...information Miguel Sena-Esteves pcDNA-SADB19L Contains rabies viral RNA polymerase; see depositing paper...

...mTurquoise II and the creation of improved fluorescent proteins through mutagenesis. Chapter 6 - The future of...Resources from the Addgene Blog Visit our Fluorescent Proteins featured topic page Read Joachim's Series on Choosing...

...The Baker Lab focuses on designing entirely new proteins, improving our understanding of how protein folding...and others. The introduction of de novo designed proteins also helped researchers understand more about ...

...CMV promoter. Envelope proteins are a key component of lentiviruses. These proteins coat the virus and help...

...Goldilocks Antibody: Guest blogger Ava Shipman explains how the Wu lab is screening over 3,000 antibodies... a drag-and-drop CRISPR editing tool for large inserts.  DAP arrays for multiplex gene editing: Guest ...

...Plasmid Kits for microbial work with fluorescent proteins, synthetic biology, gene cloning and expression...them on our search page. Use the Expression and Insert Species filters on the right-hand side of the search...

...to cut other DNA or RNA sequences. Nucleases: Proteins that bind to gRNAs and are directed by the gRNAs...prime editing and the identification of other Cas proteins continue to expand the targeting range. CRISPR...

... about Crystal’s exciting career path, to gain insight into industry research, and to get advice on how... project and scientific teams at the Novartis Institutes for BioMedical Research to develop novel biologics...

...tradition in which we choose our teams and start brainstorming months in advance. Our teams comb the news (...Friday. So if you’re interested in finding out who wins this year’s Addgene costume contest, like us on ...

... share their AAV validation data. The Data Hub contains a curated collection of community submissions ...) can save you significant time and money. For instance, this report shows that anterograde, monosynaptic...

...and metabolism. Melina has worked at Genetics Institute in Cambridge, MA and Sequana Therapeutics in San...the Addgene Blog Addgene's a nonprofit? Get an Inside Look at Addgene's NGS Quality Control Addgene's...

...academic institutions can afford to hire. It will be difficult for some of these institutions to manage...