We narrowed to 823 results for: lars;

...maintain fewer copies of plasmids if they contain large inserts or genes that create a toxic product. ...die off. Have any questions? Need something clarified? If so please, post in the comments.  References...

...protein phosphatase that responds to rising intracellular calcium levels within the cell. When levels ... wild type (top panels) and cells with high intracellular Ca2+ (bottom panels) equipped with destabilized...

...Shen, 2011). To our knowledge the effect of hyperosmolarity on AAV production yields had yet to be examined...., and Bernhard O. Palsson. "Effect of medium osmolarity on hybridoma growth, metabolism, and antibody...

... all be defined as laypeople when it comes to molecular biology.  Some lay people have a high amount of...more likely to closely read an email sent out to a large listserv or one sent to you and a few other people...

...equipment. Reporters for studying gene expression have similar drawbacks, often requiring the destruction of plants... for GUS staining or are not suited to studying large samples. RUBY-Red But recently, something new has...

...referred to as TX-TL systems) have been gaining popularity as a synthetic biology tool. These systems allow... New from the viral service By Jason Nasse We regularly add new viral aliquots from our plasmid collection...

...resistant to vaccine serum responses. This result was similar to studies that used live virus (Cele et al., 2021...neutralization rates of B.1.351 pseudovirus was similar to that of pseudoviruses representing a bat-derived...

...deposited from Di Ventura's lab, including the popular light-inducible nuclear export system (LEXY) here...page Resources on Addgene.org Check out the Molecular Biology Reference Download Addgene's Plasmids ...

...challenge in the best of cases. But it can be particularly challenging when your only antibody option is...al., 2013). One issue with this strategy is that large complexes may not permeate through your tissue samples...

...This is, for the intrepid researcher looking for molecular biology tools, a very useful trait. Virus structure... Viral vectors Viruses are quite appealing as molecular biology tools… if one isn’t too bothered by the...

...central We’re working on a project where we aim to do large scale DNA preps with multiple samples without cross...time and space in your own lab, you should give similar workshops a try if you’re ever looking to try out...

...conferences/meetings over and over to get to know the regulars (or better yet, become a volunteer). Naturally...stay in touch with all of their connections on a regular basis. It is also not at all necessary. Once you...

...sessions, not your walks between sessions Like many large conferences with multiple sessions happening at ...walking up to the mic and asking your question in a large conference room puts butterflies in your stomach...

...would take years and millions, if not billions, of dollars, but by approaching it from a new direction, we...Science Communications Team at QBI UCSF. They are particularly interested in facilitating collaborative science...

... tissue-specific promoter sounds simpler, this modular system saves you from having to create a new fly...and overexpress viral genes. Additionally, other modular expression systems exist (eg. LexA/LexAop and Cre...

...greater science literacy and education have more polarized beliefs on controversial science topics. Proceedings..., L. L., Braman, D., & Mandel, G. (2012). The polarizing impact of science literacy and numeracy on perceived...

...prime editing, achieving up to 3-loci MPE with similar high efficiencies, which is scalable for a higher...genes against multiple genetic diseases, such as muscular dystrophies, heart disease, and type 2 diabetes...

...are being given in vivo, it is difficult to use larger enzymes. To reduce the impact of off-target mutations...B. 2021. “CRISPR/Cas gene therapy.” Journal of cellular physiology.  https://doi.org/10.1002/jcp.30064...

...sensor to the membrane and displays it on the extracellular side. In addition to the original PDGFR TMD,...promoter, FLP-dependent expression GFAP: Glial fibrillary acidic protein (promoter). Good for Glial expression...

...Monoclonal antibody drugs are popular therapeutics for a plethora of disease conditions, from cancer ...Immunotoxicology Testing of Monoclonal Antibodies in Macaca fascicularis: From Manufacturing to Preclinical Studies. ...