We narrowed to 714 results for: met

1. Antibodies 101: Isotypes

   Type

   Blog Post

   Published

   Oct. 19, 2021, 1:15 p.m.

   ...in antibody-based applications including flow cytometry, immunohistochemistry, immunocytochemistry, ELISAs...

2. A Deep Dive into BioBuilder

   Type

   Blog Post

   Published

   Oct. 11, 2017, 1:44 p.m.

   ... they remember the moments when they discover something new for themselves and when they effectively contribute...

3. Addgene’s Viral Service - Why Virus? Why Now?

   Type

   Blog Post

   Published

   March 23, 2017, 2:30 p.m.

   ...forward. The viral vector project team develops methods, performs extensive quality control, generates  ...

4. Top Requested Lentivirus and AAV of 2016

   Type

   Blog Post

   Published

   Jan. 6, 2017, 3:56 p.m.

   ...genome-wide libraries for CRISPR screening. Nat Methods. 2014 Aug;11(8):783-4. PubMed PMID: 25075903. PubMed...

5. Developing a cold tolerant E. coli using specialized chaperones

   Type

   Blog Post

   Published

   Jan. 28, 2020, 2:35 p.m.

   ...2018 iGEM debut - centered around a prototypical methane biosensor - IISER Bhopal is back in the SynBio ...

6. Clear and Accessible Writing

   Type

   Blog Post

   Published

   Feb. 15, 2024, 2:15 p.m.

   ... team, working together to share information. Sometimes it is most helpful to use a lot of jargon and ...

7. CasPEDIA: A Functional Classification of Cas Enzymes

   Type

   Blog Post

   Published

   Jan. 25, 2024, 2:15 p.m.

   ... your hits, you can easily sort them by other parameters, such as E-value for protein sequence searches...

8. Sharing is Caring: But How to Distribute Open Hardware?

   Type

   Blog Post

   Published

   Feb. 28, 2023, 2:15 p.m.

   ...microscopy in the life sciences: quo vadis?. Nat Methods 19, 1020–1025 (2022). https://doi.org/10.1038/s41592...

9. Fluorescent Proteins 101: Luciferases

   Type

   Blog Post

   Published

   July 22, 2025, 1:15 p.m.

   ...Bioluminescent reporters require a chemical substrate (and sometimes cofactors) to be provided and are generally dimmer...

10. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type

    Blog Post

    Published

    Jan. 21, 2025, 2:15 p.m.

    ...of postdocs focusing on molecular biology and metabolism. He now guides fellow PhDs in their careers with...

11. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

    Type

    Blog Post

    Published

    Feb. 19, 2019, 1:36 p.m.

    ...Prior to Tufts Health Plan, Carl worked at both MetLife & Ernst & Young, LLP, auditing insurance, finance...

12. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type

    Blog Post

    Published

    Dec. 6, 2018, 1:34 p.m.

    ...efficiency of TurboID and miniTurbo makes PL a viable method for proteomics in model organisms in which it was...

13. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

    Type

    Blog Post

    Published

    Nov. 19, 2019, 2:08 p.m.

    ... of unmodified proteins. Affinity purification methods typically require a protein to be tagged, which...

14. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type

    Blog Post

    Published

    July 10, 2014, 7:17 p.m.

    ...equipment that still works. “We use functional spectrometers from the early 1990s that were free and free...

15. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type

    Blog Post

    Published

    Feb. 4, 2014, 3:50 p.m.

    ... or who your new contact will connect you to. Sometimes, it is not who you know but who knows about you...

16. dTAG - You're it!

    Type

    Blog Post

    Published

    June 21, 2018, 2:06 p.m.

    ... use of these plasmids are available here. The Methods section of Nabet et al., Nature Chemical Biology...

17. RANbodies: Reporter Nanobody Fusions

    Type

    Blog Post

    Published

    April 10, 2018, 12:56 p.m.

    ...Domain RANbody Reporters. Reporter Detection Method Uses Pros Cons Antigens HRP (P) Tyramide signal...

18. Antibodies 101: Polyclonal Antibodies

    Type

    Blog Post

    Published

    July 14, 2021, 1:15 p.m.

    ...Protein A or Protein G purification is one common method. These proteins bind the IgG heavy chain. Running...

19. All in a Twist: dsRNA

    Type

    Blog Post

    Published

    Feb. 2, 2023, 2:15 p.m.

    ...Endogenous sources of duplexed RNA DNA:RNA hybrids Sometimes ssRNA just needs a buddy, and even DNA will suffice...

20. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type

    Blog Post

    Published

    Oct. 29, 2019, 2:52 p.m.

    ... 2018) available at Addgene: MerMAID2 - A metagenomically discovered marine anion-conducting channelrhodopsin...