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  1. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ... table below lists the four common 2A peptides employed by scientists. 2A cleavage is universal in eukaryotic...reporter cloned in. In these plasmids you would simply clone your gene of interest into the multiple cloning...

  2. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...correct genetic conditions over 25 years ago when completing her PhD and medical training in Ophthalmology... kb cannot be delivered using this vector. For example, although researchers know that Usher Syndrome ...

  3. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...low RNA editing. To increase A->G editing, they employed hyperactive ADAR constructs, like ADAR2DD(E488Q...ADAR will preferentially edit an adenine if the template has a cytosine mismatch at that position. PspCas13b-ADAR2DD...

  4. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    Published
    April 1, 2025, 1:15 p.m.
    ...relied on deaminase enzymes, either to directly accomplish the base edit or to create the substrate for ...to repair the DNA using the edited strand as a template — in this case, encouraging it to incorporate ...

  5. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ... solve an issue specific to that country. ‌For example, when we began offering temperature-sensitive materials...inclusive design in our digital content. We have implemented proactive measures to find potential barriers...

  6. How to Be an Excellent Trainee

    Type
    Blog Post
    Published
    Sept. 26, 2023, 1:15 p.m.
    ...experience In research there is a saying that if the sample prep is poor, then the data will be poor. The same...you remember where the equipment, reagents, and samples are. Do: Ask your bench mentor if they have time...

  7. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ... understand the process, we’ll walk through an example experiment where we might use Gateway cloning to...into destination vectors. The cloning process is simple -  no restriction, ligation or gel purification...

  8. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    Published
    May 2, 2019, 12:41 p.m.
    ...editing in squid is possible nonetheless - the first example was of a pigmentation gene.   Genus Microtus (Zoe...Donaldson) Voles have fascinating social behavior. For example, two similar species have very different social...

  9. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    Published
    Nov. 19, 2020, 2:15 p.m.
    ...in mice is possible (Guy et al., 2007). They accomplished this using Cre-Lox mouse models in which the...Cre not only halted the neurological damage but completely reversed it, regardless of age or symptom severity...

  11. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    Published
    Oct. 19, 2017, 1:54 p.m.
    ...light inducible ion channels (plasmid # 20298 for example) have revolutionized Neuroscience. They are the...within neuronal networks in living animals. To accomplish such cell specific manipulations in vivo, FLEx...

  12. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ...avoid rousing the trolls. 3. The AMA process was simple and well organized. You can check out the r/Science...posters (this advice included leaving out the term “implicit bias” which is apparently a troll trigger phrase...

  13. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ... purified from the system, researchers can run samples on SDS-PAGE gels containing Phos-Tag, a small molecule...substitution. Alternatively, you can express the completely wild type protein from the same plasmid.  Upon...

  14. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    Published
    Jan. 26, 2016, 3:30 p.m.
    ...regularly. Dystrophin and genome editing Since complex oligonucleotide treatment comes with many challenges...therapy, it’s important to realize that DMD is a simpler case than other genetic diseases we’d like to treat...

  15. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...specific list of the candidate off-target sites. For example, if there are 10 putative off-target locations ...confounding your results should be minimal. For example, if your goal is to generate a knock-out cell line...

  16. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ...epigenetic modifications across the genome) has been implicated in disease states, and targeting the epigenome...more frequently inherited by daughter cells. For example, KRAB induced silencing was transient and quickly...

  17. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    Published
    Oct. 2, 2018, 12:56 p.m.
    ...emotions and assumptions. I’m not just talking about simple things like “Which route should I take to get to...choosing a training lab, I strongly suggest you complete a work rotation in the lab if at all possible....

  18. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...the cell to use the deaminated DNA strand as a template. To do so, they used a Cas nickase, instead of...repairs the DNA using the U-containing strand as a template, copying the base edit. Figure 2: Cytidine...

  19. New Coomassie Protocol Video

    Type
    Blog Post
    Published
    Oct. 31, 2023, 1:15 p.m.
    ...    If this video is helpful, you can find our complete collection of protocol videos on the Addgene YouTube...
Showing: 641 - 660 of 778 results