We narrowed to 794 results for: mpl

...antibody expression. If starting from a protein sample (for instance, affinity purified antibodies from...NH, Rahman MZ, He L, Xin L, Shan B, Li M (2016) Complete De Novo Assembly of Monoclonal Antibody Sequences...

...enzymes   The solution to the PAM problem seems simple, in theory: find a Cas enzyme or evolve one that...engineered Cas9 with NG PAM compatibility, as the name implies (Nishimasu et. al, 2018). xCas9 was generated by...

...CRISPR systems based on Cas9 were thought to be the simplest CRISPR systems and the easiest to adapt to genome...mechanism. To prevent new editing post-HDR, repair templates should remove the PAM sequence. Many research ...

... Reddit AMA Perhaps we should start with some examples of a bad panel discussion? You know, the one where... audience members a chance to participate. For example, at a recent event on nonprofit science organizations...

...whole outlook on the possibilities in life for employment?  It never happened – there was never a “moment... – although we didn’t figure out how we would accomplish the color change until a few months later. RevBio...

...known tropism for your cells of interest — for example, AAV8 for kidney cells — can help improve infection... away from the comfort of Cas9, and opt for a completely different Cas effector, like Cas12a (Cpf1). Even...

...administered through the tail vein — many researchers will simply refer to these as “tail vein injections.” However...depending on researcher preferences. It sounds complicated but has a pretty low learning curve! Restraint...

...procedure (illustrated in the YouTube video below) is simple: After obtaining antibiotic-resistant colonies,...initially, or using secondary biological screens. For example, for cancer researchers, if growth properties in...

...Compared to humans and mice, flies have a relatively simple genome. They have four chromosomes: the X (and ... dominant phenotypes visible on the flies. For example, second chromosome balancers often have the curly...

...in the form of mRNA for academic research. The template plasmids for generating your own libraries are...the end of the mRNA molecule, forming a ribosome complex consisting of the translated protein (sybody), ...

...just by looking at the cells on the plate. For example, if your plasmid contains a gene encoding a blue...after transforming them into your cells, you can simply look for blue colonies to determine which cells...

...people involved is staggering. The process is complicated. If you’re interested in the drug discovery process...postdocs and do not support or manage them well are simply no longer allowed to have postdocs. In academia...

...attention on social media. I gained a concrete example to use to promote future attempts and learned a...roles of scientists outside of academia One recent example: Don't call me a dropout: Why science needs more...

... table below lists the four common 2A peptides employed by scientists. 2A cleavage is universal in eukaryotic...reporter cloned in. In these plasmids you would simply clone your gene of interest into the multiple cloning...

...correct genetic conditions over 25 years ago when completing her PhD and medical training in Ophthalmology... kb cannot be delivered using this vector. For example, although researchers know that Usher Syndrome ...

...low RNA editing. To increase A->G editing, they employed hyperactive ADAR constructs, like ADAR2DD(E488Q...ADAR will preferentially edit an adenine if the template has a cytosine mismatch at that position. PspCas13b-ADAR2DD...

...relied on deaminase enzymes, either to directly accomplish the base edit or to create the substrate for ...to repair the DNA using the edited strand as a template — in this case, encouraging it to incorporate ...

... solve an issue specific to that country. ‌For example, when we began offering temperature-sensitive materials...inclusive design in our digital content. We have implemented proactive measures to find potential barriers...

...light inducible ion channels (plasmid # 20298 for example) have revolutionized Neuroscience. They are the...within neuronal networks in living animals. To accomplish such cell specific manipulations in vivo, FLEx...

... understand the process, we’ll walk through an example experiment where we might use Gateway cloning to...into destination vectors. The cloning process is simple -  no restriction, ligation or gel purification...