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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 789 results for: mpl

Showing: 641 - 660 of 789 results

  1. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    Published
    Oct. 22, 2014, 1:57 p.m.
    ... Reddit AMA Perhaps we should start with some examples of a bad panel discussion? You know, the one where... audience members a chance to participate. For example, at a recent event on nonprofit science organizations...

  2. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    Published
    June 5, 2014, 3:32 p.m.
    ...whole outlook on the possibilities in life for employment?  It never happened – there was never a “moment... – although we didn’t figure out how we would accomplish the color change until a few months later. RevBio...

  3. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...people involved is staggering. The process is complicated. If you’re interested in the drug discovery process...postdocs and do not support or manage them well are simply no longer allowed to have postdocs. In academia...

  4. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    Published
    Sept. 7, 2017, 12:17 p.m.
    ...procedure (illustrated in the YouTube video below) is simple: After obtaining antibiotic-resistant colonies,...initially, or using secondary biological screens. For example, for cancer researchers, if growth properties in...

  6. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    Published
    June 18, 2020, 1:15 p.m.
    ...in the form of mRNA for academic research. The template plasmids for generating your own libraries are...the end of the mRNA molecule, forming a ribosome complex consisting of the translated protein (sybody), ...

  7. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ...known tropism for your cells of interest — for example, AAV8 for kidney cells — can help improve infection... away from the comfort of Cas9, and opt for a completely different Cas effector, like Cas12a (Cpf1). Even...

  8. Common Injection Routes in Mice

    Type
    Blog Post
    Published
    Jan. 22, 2025, 9:42 p.m.
    ...administered through the tail vein — many researchers will simply refer to these as “tail vein injections.” However...depending on researcher preferences. It sounds complicated but has a pretty low learning curve! Restraint...

  10. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    Published
    Feb. 25, 2016, 3:30 p.m.
    ...attention on social media. I gained a concrete example to use to promote future attempts and learned a...roles of scientists outside of academia One recent example: Don't call me a dropout: Why science needs more...

  11. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ... table below lists the four common 2A peptides employed by scientists. 2A cleavage is universal in eukaryotic...reporter cloned in. In these plasmids you would simply clone your gene of interest into the multiple cloning...

  12. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...correct genetic conditions over 25 years ago when completing her PhD and medical training in Ophthalmology... kb cannot be delivered using this vector. For example, although researchers know that Usher Syndrome ...

  13. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...low RNA editing. To increase A->G editing, they employed hyperactive ADAR constructs, like ADAR2DD(E488Q...ADAR will preferentially edit an adenine if the template has a cytosine mismatch at that position. PspCas13b-ADAR2DD...

  14. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    Published
    May 2, 2019, 12:41 p.m.
    ...editing in squid is possible nonetheless - the first example was of a pigmentation gene.   Genus Microtus (Zoe...Donaldson) Voles have fascinating social behavior. For example, two similar species have very different social...

  15. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    Published
    Oct. 19, 2017, 1:54 p.m.
    ...light inducible ion channels (plasmid # 20298 for example) have revolutionized Neuroscience. They are the...within neuronal networks in living animals. To accomplish such cell specific manipulations in vivo, FLEx...

  16. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ... understand the process, we’ll walk through an example experiment where we might use Gateway cloning to...into destination vectors. The cloning process is simple -  no restriction, ligation or gel purification...

  17. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    Published
    Nov. 19, 2020, 2:15 p.m.
    ...in mice is possible (Guy et al., 2007). They accomplished this using Cre-Lox mouse models in which the...Cre not only halted the neurological damage but completely reversed it, regardless of age or symptom severity...

  18. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ... solve an issue specific to that country. ‌For example, when we began offering temperature-sensitive materials...inclusive design in our digital content. We have implemented proactive measures to find potential barriers...

  19. How to Be an Excellent Trainee

    Type
    Blog Post
    Published
    Sept. 26, 2023, 1:15 p.m.
    ...experience In research there is a saying that if the sample prep is poor, then the data will be poor. The same...you remember where the equipment, reagents, and samples are. Do: Ask your bench mentor if they have time...

  20. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    Published
    April 1, 2025, 1:15 p.m.
    ...relied on deaminase enzymes, either to directly accomplish the base edit or to create the substrate for ...to repair the DNA using the edited strand as a template — in this case, encouraging it to incorporate ...
Showing: 641 - 660 of 789 results