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Western blots have many technical elements. We cover each element, and the different options available to choose from, in this post.

Aug. 22, 2024, 1:15 p.m.

Did you know CRISPR can be used to target non-coding RNAs? In this blog post, we explain the pros and cons of different approaches.

Nov. 14, 2024, 2:15 p.m.

Find the basics about chemogenetics: what are chemogenetics, why use them, and how to build your chemogenetic system using receptors and ligands.

July 11, 2023, 1:15 p.m.

An in-depth look at fixing and permeabilizing samples in immunofluorescence experiments.

Aug. 30, 2022, 1:15 p.m.

an overview of qPCR and ddPCR copy number variant assays.

April 26, 2022, 1:15 p.m.

Our new Instagram series, Meet an Addgenie, features stories form Addgenies about their career decisions, paths, and more.

June 25, 2020, 1:15 p.m.

A new open source tool for CRISPR experiments, CrispyCrunch, has been developed by the Chan Zuckerberg Initiative to design batch gRNAs, templates, and sequencing primers for CRISPR + HDR experiments.

Feb. 7, 2019, 2:16 p.m.

Learn how a 3D protein model can help you visualize, learn, and teach others about protein structure and function.

Oct. 17, 2017, 12:55 p.m.

Get your fly research started with this quick guide to working with drosophila.

July 13, 2017, 2:30 p.m.

Having trouble with your transductions? Find troubleshooting tips from Addgene scientist Leila Haery.

Aug. 11, 2016, 2:23 p.m.

Tips to help you choose which fluorescent protein to use in your experiments.

May 20, 2014, 2:06 p.m.

Learn about the advantages of using AAV as a delivery method for CRISPR screens.

Feb. 4, 2025, 2:15 p.m.

In this blog post, we walk you through how to create and use a serial dilution in your experiments.

Aug. 15, 2024, 1:15 p.m.

Learn about the structure and life cycle of retroviruses to help understand how to use viral vectors tools in the lab.

July 27, 2023, 1:15 p.m.

Two new CRISPR tools from the Abudayyeh-Gootenberg lab are now available at Addgene: Cas7/11 and PASTE.

Feb. 17, 2022, 2 p.m.

An introduction to the use of antibodies in immunoprecipitation, including antibody immobilization, controls, and a basic overview of techniques.

Dec. 7, 2021, 2:15 p.m.

Choosing a CRISPR activator? This article features four of the most popular CRISPR activators (dCas9-VP64, SAM, SunTag, and VPR) and some newer activators like dCas9-CBP and SPH.

Oct. 6, 2020, 1:15 p.m.

Marc Zimmer's book, The State of Science, examines broad issues in science including racism and sexism, peer-review, funding, CRISPR, and the politicization of science.

July 30, 2020, 1:15 p.m.

Learn all about CAPTURE - a new CRISPR-based technique to study regulatory elements in their native chromatin contexts.

July 2, 2020, 12:40 p.m.

Find tips and tricks for using digital droplet PCR to quantify your AAV titers.

Jan. 16, 2020, 2:18 p.m.