We narrowed to 711 results for: tat

1. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

   Type

   Blog Post

   Published

   Nov. 5, 2019, 1:28 p.m.

   ...approaches, knowledge of both the advantages and limitations of each Cas enzyme can help determine which enzyme...

2. Early Career Researcher Toolbox: Social Media for Scientists

   Type

   Blog Post

   Published

   Dec. 11, 2018, 12:46 p.m.

   ...the three social media platforms, there’s some statistics on what percent of scientists and engineers regularly...

3. How to Make Friends and Meet People at a Scientific Conference

   Type

   Blog Post

   Published

   Aug. 7, 2014, 1:58 p.m.

   ...exhausted that you can’t get a lot from the presentations and programs. After the conference Follow ...

4. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

   Type

   Blog Post

   Published

   July 10, 2014, 7:17 p.m.

   ... to achieve success in this business. A good presentation can help win a business plan competition and...

5. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

   Type

   Blog Post

   Published

   March 3, 2020, 2:15 p.m.

   ...further tested the base editors by correcting a mutation that causes Niemann-Pick disease type C, directly...

6. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

   Type

   Blog Post

   Published

   April 23, 2018, 2 p.m.

   ...modeling. Its reversibility allows precise and quantitative analysis of epigenetic memory at nearly any ...

7. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

   Type

   Blog Post

   Published

   Jan. 31, 2019, 1:32 p.m.

   ...let’s take a look at exosomes. Proficient in transportation, exosomes are tools of communication and provide...

8. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

   Type

   Blog Post

   Published

   Aug. 5, 2021, 12:15 p.m.

   ...painful stimuli are processed by a network of excitatory and inhibitory neurons whose function and connectivity...

9. Antibodies 101: Polyclonal Antibodies

   Type

   Blog Post

   Published

   July 14, 2021, 1:15 p.m.

   ...protein in low abundance or whose conformational state may change. If your protein might become slightly...

10. CRISPRainbow and Genome Visualization

    Type

    Blog Post

    Published

    Feb. 28, 2017, 3:30 p.m.

    ...fluorescently labeled Cas9’s come with a number of limitations. Since color is specified by PAM sequence, each...

11. Antibodies 101: Conjugation

    Type

    Blog Post

    Published

    March 26, 2024, 1:15 p.m.

    ...Adler M, Wacker R (2007) Detecting antigens by quantitative immuno-PCR. Nat Protoc 2:1918–1930. https://...

12. To Each HIS Own

    Type

    Blog Post

    Published

    March 2, 2023, 2:15 p.m.

    ... H, Gentz R and Stüber D. Genetic Approach to Facilitate Purification of Recombinant Proteins with a Novel...

13. Plasmids 101: The protein expression toolbox

    Type

    Blog Post

    Published

    Feb. 16, 2023, 7:05 p.m.

    ... gRNA is all you need to generate a frameshift mutation that will permanently ablate protein expression...

14. All in a Twist: dsRNA

    Type

    Blog Post

    Published

    Feb. 2, 2023, 2:15 p.m.

    ...inflammatory response. However, if dsRNA sensors are mutated or mechanisms which suppress endogenous dsRNA formation...

15. Giving gRNAs a Facelift - Synthetic and Beyond

    Type

    Blog Post

    Published

    Oct. 20, 2022, 1:15 p.m.

    ...as the unnecessary stress of worrying about the status of your RNA. gRNAs modifications are even going...

16. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type

    Blog Post

    Published

    Dec. 8, 2016, 3:30 p.m.

    ...actually contain your insert of interest. Simply stated, ccdB makes cloning easier by selecting against...

17. Developing Lab Management Software for Biology

    Type

    Blog Post

    Published

    Aug. 25, 2016, 2:30 p.m.

    ... track the location, quality, growth, and legal status of thousands of plasmids a day, like we do at Addgene...

18. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type

    Blog Post

    Published

    July 21, 2016, 2:30 p.m.

    ...Gendered Innovations. This project: 1) develops state-of-the-art methods of sex and gender analysis and...

19. Adenoviral Vector Production and Troubleshooting

    Type

    Blog Post

    Published

    June 14, 2018, 11:44 a.m.

    ...ratio for the purified virus should be ~1.3 Quantitative PCR: the number of viral DNA packaged in virions...

20. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type

    Blog Post

    Published

    Sept. 26, 2019, 12:50 p.m.

    ... The team found that "all-in-one" Nanoblades facilitated HDR with either a ssDNA or dsDNA donor template...