We narrowed to 813 results for: tes
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TypeBlog PostPublishedJuly 27, 2023, 1:15 p.m....Figure 3: The life cycle of a retrovirus. Image courtesy of Jeremy Luban, MD. Figure 3 depicts the ... the National Academy of Sciences of the United States of America, 54(1), 137–144. https://doi.org/10.1073...
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AAVs in Retinal Gene Therapy
TypeBlog PostPublishedJuly 31, 2018, 12:55 p.m....results in preclinical studies some potential candidates for rare to ultra-rare ocular diseases are being... of new capsid variants, designing expression cassettes to regulate and optimize transgene levels, and... -
Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!
TypeBlog PostPublishedDec. 3, 2019, 11:38 a.m.... ‘Vector Types’ I selected ‘Lentiviral’ which eliminates most of the plasmids designed for other uses....guide RNA / shRNA inserts Resistance Marker: This indicates the selectable marker used in your model system... -
SciComm: Taking Science to Elementary and Middle Schools
TypeBlog PostPublishedJuly 9, 2019, 12:52 p.m....study by the NSF showed that among US 4th graders tested on the Science National Assessment of Educational... you don’t have children but are interested in testing your SciComm skills or are interested in a career... -
Deep Dive: Fixing and Permeabilizing for Immunofluorescence
TypeBlog PostPublishedAug. 30, 2022, 1:15 p.m....within the folded protein (green region). Methanol dehydrates the protein, which can disrupt protein structure...at your antibody datasheet to see if it has been tested in IF and if a protocol is available. When possible... -
Summer SciComm Series: Modes of Communication
TypeBlog PostPublishedJuly 1, 2022, 1:15 p.m....longer educational videos, and Twitter, for sharing updates, new deposits, and new blog posts! How to... Math, sarcasm, romance, and spacecraft! Image courtesy of xkcd.com Science communication should be... -
Tips for Writing a Good Cover Letter
TypeBlog PostPublishedMay 21, 2024, 1:30 p.m....for labs and nonprofits, who tend to prioritize candidates that are excited about their work and mission... will be close to that of a professional email. Notes from real-life hiring managers In preparation for... -
Analyzing CRISPR Editing Results with ICE from Synthego
TypeBlog PostPublishedMay 8, 2018, 1 p.m....checked circle. Typically, a yellow check mark indicates that a particular parameter was automatically ...sequencing bases downstream of the cut. Summary ICE generates NGS-quality CRISPR editing analysis from Sanger... -
AAV Titers: Where Do They Come From And What Do They Mean?
TypeBlog PostPublishedNov. 15, 2017, 3 p.m....particles (black arrowhead). Scale bar = 100nm. Image courtesy of David Bell and Svetla Stoilova-McPhie, Center...assay. This may be due to competition for binding sites between the primers and the AAV genome repeats. ... -
Transferable Skills Guide: Career Planning Resources
TypeBlog PostPublishedNov. 14, 2017, 2 p.m....of recruiters for identifying appropriate job candidates. If you do receive such a call, make sure to ...-out-of-job-fairs-2014-3 https://www.forbes.com/sites/neilpatel/2015/05/21/15-surprising-rules-you-should-know-regarding-linkedin-etiquette... -
Viral Vectors 101: Pseudotyping
TypeBlog PostPublishedApril 20, 2021, 2:30 p.m....transduction of primary lymphocytes and CD34+ cells derived from human and nonhuman primates. Blood 100:823–832... -
Healthcare Consulting: A Door to the Business of Life Sciences
TypeBlog PostPublishedOct. 13, 2016, 2:30 p.m....preclinical and clinical research to discover and test the best new therapeutics. Consultants often work...PhDs, and an increasing recognition of the value graduates bring to these roles. Healthcare consulting is... -
Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs
TypeBlog PostPublishedOct. 27, 2015, 3 p.m.... Jennifer Lippincott-Schwartz at the National Institutes of Health and he is currently an Associate Professor...Paladino, Simona, et al. "Protein oligomerization modulates raft partitioning and apical sorting of GPI-anchored... -
Data Freedom: The Expansion of Data Sharing in Research Publications
TypeBlog PostPublishedAug. 5, 2014, 6:51 p.m....others understand ours. Improving access to data promotes dissemination, especially for young researchers...useful to others and efforts to enhance these attributes should be welcomed. However, expectations must... -
Antibodies 101: Normalization and Loading Controls for Western Blots
TypeBlog PostPublishedApril 8, 2025, 1:15 p.m....total protein are compared. While a higher slope indicates higher sensitivity, linearity is the most important... than the total protein quantification. This illustrates why total protein measurement is often more accurate... -
Quick Guide to Near-Infrared Fluorescent Proteins
TypeBlog PostPublishedOct. 5, 2021, 1:15 p.m....neurons in the neocortex of a living mouse. Image courtesy of Axel Nimmerjahn (Salk Institute for Biological...tags due to the proximity of the N- and C-termini. Latest miRFPnanos9 do not yield to (m)iRFPs in cellular... -
Five Popular Model Organisms
TypeBlog PostPublishedApril 11, 2019, 12:39 p.m....misfolding that leads to a build-up of protein aggregates termed “lewy bodies” in the brain. Yeast models...majority of C. elegans are also self-fertile hermaphrodites making it easy to conduct large experiments... -
Harnessing Bacterial Toxins for Allelic Exchange
TypeBlog PostPublishedAug. 15, 2019, 12:30 p.m....of toxins before the second crossover step, they tested their expression under both rhamnose- and arabinose-inducible...selective RNA unfolding and sliding at upstream standby sites." Nucleic acids research42.4 (2013): 2646-2659. ... -
CRISPR 101: Validating Your Genome Edit
TypeBlog PostPublishedNov. 3, 2022, 12:15 p.m....doesn’t affect any endogenous restriction enzyme sites? Never fear! You can introduce a silent, passenger...CRISPRitz or CRISPOR can help narrow down the list of sites where off target editing may occur, allowing you... -
Using Phosphoserine to Study Protein Phosphorylation
TypeBlog PostPublishedJune 23, 2016, 2:30 p.m.... for the majority of documented phosphorylation sites. If knowledge of a candidate regulatory kinase is...that binds specifically to phosphate moieties and promotes a robust mobility shift in phosphorylated, but...