...of prominent members of the bioscience and tech transfer communities who help guide and promote Addgene...overall mission of making science easier and more transparent. That’s largely my job [at the Broad] too… to...role in the future, Doench says, Addgene is “a central resource for what is worth having. The work that...critical function really…. Addgene’s role as a central repository for what is useful and what was used...

...from a dcm or dam methylation-deficient bacterial strain such as JM110 or INV110. Avoid star activity. Some...DNA and allows you to visualize the DNA under ultraviolet (UV) light. Don’t forget to add loading buffer... estimate how far down the gel your bands have traveled based on the dye! Always run a ladder. Ladders...of larger DNA fragments. I hope these tips demonstrate that plasmid verification is not just necessary...

...Washington DC (#SfN17). This massive conference attracted over 30,000 attendees (scientists and nonscientists...sampling of the broad variety of tools in neuroscience trade. There were, of course, many companies selling antibodies...people ready to discuss their VR helmets, gait tracking technologies, and brain monitors. Walking around...experiments and to find tools that will make your transition into the field much easier! If there’s a plasmid...

...research more visibility.    Figure 1: Illustration of SARS-CoV-2 from Philippa Steinberg for the...glycoproteins and other proteins that require post-translational modification may not be easily expressed in ...sequencing as a first pass QC. The lab team manually transforms, innoculates, and performs DNA minipreps for ...order to bring them inline with our slower, more traditional, QC process. While this has worked well for the...

... host cells (the simplest example would be a transformed mesophilic, for example E. coli, carrying a plasmid...higher levels of growth compared to the E. coli strain expressing the empty vector. And thus was born ...working towards widening the applicability of our strategy using the pMBP-LbCas12a and pC013-Twinstrep-SUMO-huLwCas13a...blogger Sreepadmanabh M from IISER Bhopal. All illustrations / figures featured were designed by our team...

...26343581. PubMed Central PMCID: PMC4597191. 5. Eyquem, Justin, et al. "Targeting a CAR to the TRAC locus with...the Memorial Sloan Kettering Cancer Center. In contrast to using randomly integrating viral vectors, CRISPR...(2009): 110-116. PubMed PMID: 19122659. PubMed Central PMCID: PMC2671237. Additional Resources on the ...

... alternative cloning strategies There are many strategies beyond the traditional PCR/digest/ligate methods... production genome wide screens and the more traditional “one at a time” approach. According to his math...

...raised or hours tracked, 3% of postdocs were at institutions focused only on tracking hours, and 22% of...that institutions have sent, with the lack of transparency and poor communication of their plans (many ...postdocs at the University of California, who had a contract - negotiated by their union - securing higher ...

...in the US and Western Europe have taken three strategic approaches to gender equality over the past several...an international research team from Norway and Australia worked with stem cells in mice. They appropriately...female stem cells—this was an unconscious and arbitrary decision. Unfortunately, it also meant that, by... Scientific Impact Are Evoloving Check Out Extracurricular Activities for a Strong Science Career Path...

...will allow you to set the amount of transcriptional and translational activity a cell has and control how...actually were not many hurdles. It was pretty straightforward surprisingly. So I think a nice thing about...on NOR gates. It’s a fundamental unit. We did demonstrate that you can use the CRISPR chemistry for a NOR...

...Gate cloning, perform an exonuclease cleanup, transform, plate, and pick. In short, the Fragmid system...AAV pRDA_889 (ITRs from AAV2) Mammalian Transposon pRDA_757 (Piggybac; constitutive expression...   Drosophilia Plasmid pRDB_053 (for transgenic flies; gypsy insulator sequences; white + selectable... drosophila) Delivery type (lentivirus, AAV, transposon, Plasmid) Destination vector (dropdown options...

...need for gene editing in research, the old PAM constraints just won’t cut it anymore. Say hello to PAM-free... evolve one that uses a PAM with no sequence constraints, or at least has looser or different requirements.... The activity of SpRY was validated in double stranded cleavage and base editing applications, with high...Plasmids available here) Walton, R. T., et al., Unconstrained genome targeting with near-PAMless engineered...

...tools used were key for ultra-high-complexity assembly. While the traditional rules of thumb for overhang...Potapov et al., 2018), which showed that the five traditional rules didn’t need to be strictly followed in ...sets (with overhangs that violate some of the traditional design rules) for successful high-complexity ...

...that the TadA domain can edit the short single-stranded DNA stretch that is accessible. Image from Gaudelli...used a previously developed bacterial selection strategy (Gaudelli et al., 2017) in combination with a ...dC9-ABE8.x-m/d). The dC9-ABE8.x-m/d constructs demonstrated a 2.1x on-target DNA-editing efficiency in a...attribute for therapeutic application. ABE8s demonstrate an overall improved base editing capacity, even...

...which was trained on key factors from past PubMed user searches, such as clicks to read the abstract or full...publications and figures using machine learning to extract important features such as influential citations...the Chan Zuckerberg Initiative. Meta is not a traditional search engine. Instead, you create your own research...

...constantly moving so we need good ways to monitor and track them. We have a number of microscopes, including...meaning that we can obtain known mutants at $7.00 a strain. Makerspaces like ours can also make accounts with...motor behaviour, and ultimately submit our new strains back to the CGC for other researchers to use. Although...money is always tight. Of course, unlike your traditional biology lab, we have members with a wealth of...

...contaminated.Learn how Addgene uses barcodes to track samples Keep track of passage numbers Keep an eye on the ...too young and should not be used for important transfection experiments right after they’ve been thawed....9(6): p. e98635. PubMed PMID: 24901218. PubMed Central PMCID: PMC4047077. 2. Pagliara, Patrizia, et al...

... approaches (Vu et al., 2024). Their optimized strategy uses new combinations of PE protein components...NC: nucleocapsid RNA chaperone, RT: reverse transcriptase. Right: T1 tomato plants that inherited an edited...then used single-molecule FRET to study the intramolecular dynamics of the proteins in solution and when...-dependent and -independent versions that are trafficked to the soma via the Kv2.1 targeting motif as ...

... because the system modulates protein post-translationally, unlike many other methods. Secondly, these...proteins. Thirdly, degrons and their drugs are titratable. With these tags, protein levels aren’t limited...limited to ‘on’ and ‘off’; varied levels of drug concentration can yield intermediate protein abundance. Finally... like the SMASh tag does. However, it is a straightforward system – HaloTag and its PROTAC are all that...

...shown them how to isolate DNA from a strawberry, or demonstrated that they’re living in an exciting, tumultuous... think that establishing this history helped demonstrate that a) I was a real person who used to be a ...become public in the previous two weeks, which demonstrated to the students that scientific history was ...