We narrowed to 694 results for: ust

1. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

   Type

   Blog Post

   Published

   July 2, 2020, 12:40 p.m.

   ...chromatin interaction networks since they are a large cluster of enhancers. CAPTURE 2.0’s multiplexing and high...

2. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

   Type

   Blog Post

   Published

   June 18, 2020, 1:15 p.m.

   ...some other proteins. The Seeger lab developed a robust in-vitro protocol that enables rapid selection ...

3. xCas9: Engineering a CRISPR Variant with PAM Flexibility

   Type

   Blog Post

   Published

   March 28, 2018, 6:52 p.m.

   ...targeting should be easy, right? Yes and no. If you’re just trying to create a knockout, you might be happy ...

4. Truncated gRNAs for Regulating Gene Expression

   Type

   Blog Post

   Published

   Jan. 10, 2017, 3:37 p.m.

   ...capable of recruiting a transcriptional activator, robust regulation of gene expression is observed in the...

5. The Scientific Conference Poster Session: Tips for Success

   Type

   Blog Post

   Published

   Aug. 29, 2019, 12:50 p.m.

   ...don’t hesitate to be honest and tell your audience just that. Explain what you do know about a related topic...

6. AAV Vector Quality Control: Going the Extra Mile with NGS

   Type

   Blog Post

   Published

   Sept. 12, 2017, 1:44 p.m.

   ...addition to genome identification, we developed custom open-access Python scripts that can perform a deeper...

7. Plasmids 101: 5 factors to help you choose the right cloning method

   Type

   Blog Post

   Published

   Aug. 21, 2018, 12:31 p.m.

   ...restriction sites allowing you to easily create customized overhangs to assemble up to 6 different fragments...

8. Plasmids 101: Optimizing Plasmid Yields

   Type

   Blog Post

   Published

   May 26, 2016, 2:30 p.m.

   ...LB broth and antibiotic concentrations will work just fine; however, for low copy plasmids or slower growing...

9. Light Sheet Fluorescence Microscopy

   Type

   Blog Post

   Published

   July 16, 2015, 2:30 p.m.

   ...such as in the Ultramicroscope.  In addition, adjusting the thickness of the laser sheet and using multi-view...

10. Plasmids 101: Multicistronic Vectors

    Type

    Blog Post

    Published

    Sept. 9, 2014, 8:20 p.m.

    ... IRES fluorescent reporter for easy detection.5  Just released: Addgene's Plasmids 101 eBook And now for...

11. Which Fluorescent Protein Should I Use?

    Type

    Blog Post

    Published

    May 20, 2014, 2:06 p.m.

    ...Victoria. Since that discovery, the family of FPs just keeps getting larger with hundreds of variants available...

12. Viral Vectors 101: The Retroviral Lifecycle

    Type

    Blog Post

    Published

    July 27, 2023, 1:15 p.m.

    ...virus’ reproductive cycle is the fact that the virus must have its structural proteins expressed from a reverse-transcribed...

13. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type

    Blog Post

    Published

    July 16, 2020, 1:15 p.m.

    ...is that our dataset contains plasmid orders from just one lab, which limits how broadly one can interpret...

14. A Guide to Starting Your Own Journal Club

    Type

    Blog Post

    Published

    Feb. 6, 2020, 3 p.m.

    ...cutting edge science, researchers of all varieties must stay current with literature and emerging technologies...

15. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type

    Blog Post

    Published

    Sept. 28, 2017, 2:01 p.m.

    ...transactivator) is co-injected. The dose of this AAV can be adjusted to achieve sparse levels of labeling, without ...

16. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type

    Blog Post

    Published

    Dec. 7, 2020, 2:53 p.m.

    ... recognized during transposition, genetic cargos must be flanked by the L and R ends - thus, scarless ...

17. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

    Type

    Blog Post

    Published

    Aug. 27, 2020, 1:15 p.m.

    ...BiFC Experiment Much like FRET, BiFC experiments must be carefully optimized to be successful. A BiFC ...

18. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type

    Blog Post

    Published

    Sept. 8, 2016, 2:30 p.m.

    ...PubMed PMID: 26575238. 5. Crivat, Georgeta, and Justin W. Taraska. "Imaging proteins inside cells with...

19. Lentiviral Vector Uses and Overview

    Type

    Blog Post

    Published

    May 19, 2016, 2:30 p.m.

    ... could infect non-dividing cells, but without sustained transgene expression. To design a viral vector...

20. Cpf1: A New Tool for CRISPR Genome Editing

    Type

    Blog Post

    Published

    Oct. 14, 2015, 2:30 p.m.

    ...candidates, Zetsche et al. found two that display robust cleavage activity similar to that of Cas9. These...