...plasmids deposited at Addgene from the abstract page. Check out that beautiful Addgene “Find Plasmids” badge...LinkOut” and search for “Addgene”. Then check the “Link Icon” box next to the “Addgene Nonprofit plasmid repository...Manuscript Resources on Addgene.org Learn How to Order Plasmids from Addgene Check Out Our FAQ Pages ...badge to be taken directly to the plasmids on Addgene’s site (Side note: I always recommend you read the...plasmids badge appear So how do you make this magical Addgene badge appear? First you’ll need to create an account... hope this helps! Additional Resources on the Addgene Blog Get Tips on Keeping Up with the Literature... addgene...

...expand Addgene’s services. There was an overwhelmingly positive response to the option of Addgene providing...allows us to maintain Addgene’s high quality standards?” Under the leadership of Addgene’s co-founder, Melina...In the middle of 2016 Addgene started distributing a small but growing catalog of ready-made AAV and ...Lentiviral preps. This new Viral Service represents Addgene’s largest new initiative since we started distributing... capabilities to produce and distribute virus. Addgene is a 501(c)3 nonprofit and our plasmid service ...build a completely self-supporting service so that Addgene is not dependent on grants. Aiming to operate under...Sponsors and Supporters Page Thank you to all of our generous, sharing depositors. Over 3,000 laboratories are...

... orientation as the gene or use custom gene synthesis or site directed mutagenesis to remove the BbvCI...this. 2) When your gene of interest has a BbvCI site(s). If so, the nicking mutagenesis protocol will still...work if the orientation of the BbvCI site(s) of the gene are in the same orientation as those in the plasmid...Additional Resources on the Addgene Blog Use Site Directed Mutagenesis to Generate Specific Mutations in Your... pot saturation mutagenesis as a quick and easy technique that can be used to generate complex libraries...of this. Plasmid library generation prior to one pot saturation mutagenesis Several methods can be used...effects. One-pot saturation mutagenesis The one-pot saturation mutagenesis technique described in Wrenbeck...

...Versatile Tool for Gene Expression in Mammals Additional Resources on Addgene.org Overview of Viral ...pooled libraries. Whether you’d like to knockout a gene, replace it, modulate its expression, or perform...while rtTA can be used to activate expression of a gene under the control of the tet operator. AAV AAVs ...associated virus (AAV, primarily chemogenetics tools for now but with optogenetic tools coming soon). The viral...excited that Addgene is now offering virion preparations to the scientific community. Addgene's AAV preparations...posts on the blog and pages on the Addgene website. blog.addgene.org The blog provides tips for working...email us and we’ll get you writing!     www.addgene.org On the Addgene website you’ll find our updated viral...

...which NANOG-related genes are critical to stem cell survival. Knowing which genes influence the survival...compaction, an enhancer can increase the expression of a gene that is millions of base pairs away. (For details...GPS for the genome” to compute coordinates for genetic elements like protein, RNA, and DNA (Table 1). ...family as HIV. By design, our lentivirii are genetically programmed to CRISPR out the enhancers we identified...differentiate over time, we periodically use next-generation sequencing to measure the relative proportion...pillar and the ESC dies. Order Lentivirus from Addgene If you’re interested in designing your own CRISPR...Hypersensitive Sites and Intergenic CRISPRs Yours truly generated the DHS data set by starting with all of the DHS...

... Figure 1: In conventional gene targeting (A), you must first generate your desired mutation in mouse...mutations, the 8-10 month process of conventional gene targeting is lowered to about 2-3 months. But certain...example of inserting a 2A-FlpO cassette into the Fgf8 gene. Miura et al. (2018) suggest using a 50-80 bp sequence... mouse model generation easi-er! Generating mice before CRISPR The process of generating knock-in mice...process of mouse model generation simpler, as described in an earlier post on generating CRISPR mouse models...after editing. How to use Addgene plasmids for Easi-CRISPR You can use Addgene plasmids to create a repair...template. Figure 2: Overview of ivTRT to generate an ssDNA repair template. Although effective...

...the newest member on Addgene’s Board of Directors, Bart Newland. Newland is General Counsel at Atalanta... is developing RNAi therapeutics to treat neurodegenerative diseases. He has earned a B.S. in microbiology...like? BN: I've made the transition now to being general counsel of a small start-up. For the last 20 years...and Biogen. They’re very different jobs. Being general counsel at a small start-up is very interesting...interesting. It’s very varied. There’s IP work to do, general corporate work, and negotiating contracts with other...all. Q: How does your experience help you as an Addgene board member? BN: Having seen so much in the business.... I think these are things that I can bring to Addgene -- that comfort with saying, “if we do this, then...

... chances are you are a fan of Addgene or our very cute mascots!  Blugene has been helping us make Plasmids...default backgrounds all year and want a change? Addgene has you covered. Today we’re releasing 11 bold ... purple bacteria video background with mascots Blugene and Aavery? Download the 720p version or download...might want to use one of our virus backgrounds. Addgene’s viral service provides ready-to-use AAV and lentiviral...the joke? If not, you might want to check out Addgene’s Fluorescent Proteins blog topic to learn more ...background.     You can find all of the plasmids that Addgene offers by visiting our collections page. Here, ... green DNA background.     Since we ❤️ DNA at Addgene, we're presenting you with another DNA background...

... first hear about Addgene?  I heard about Addgene from Joanna Brownstein, an Addgene board member. When...resources on the Addgene blog Learn more about about Addgene in our "Inside Addgene" blog series Subscribe...Subscribe to the Addgene blog Resources on addgene.org: Meet the other members of Addgene's Board of Directors...We’re excited to welcome Julie Cicalese to the Addgene Board of Directors. Cicalese served as the Chief...experience and insight to the now 13 member board at Addgene. Let’s meet the newest board member and get to ...School, Joanna was my colleague. She mentioned Addgene was looking for someone with HR expertise to join...serves as an objective body to oversee some of Addgene’s compensation decisions. It was a great time for...

...delivery enables minimally-invasive gene delivery and optogenetic excitation. Excitation of neurons deeper...the differences between chemogenetics and optogenetics Resources on Addgene.org Browse all viral vectors...available at Addgene Visit our viral vectors guide Find our chemogenetics and optogenetics science guides...resources on the Addgene blog Read more about the PHP.eB AAV capsid Read more about optogenetics Learn about...Optogenetics is a neuroscience method that lets you fire neurons with the flick of a light switch. Neurons...allowing an influx of ions into the neuron which generates an action potential (Figure 1). This light-induced... to use invasive measures to perform in vivo optogenetic experiments. Intracranial injections of AAV are...