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TypeBlog PostPublishedOct. 22, 2014, 1:57 p.m....panelist or audience member from taking over. My own personal rule is that I do not let the panelists introduce...stop talking and redirect the conversation. The person you cut off might be miffed but the other 200 audience...
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The Scientific Conference Poster Session: Tips for Success
TypeBlog PostPublishedAug. 29, 2019, 12:50 p.m....to capitalize on your experience. No matter the reason for attending, your preparation and day-of game...hear or understand what we are being asked. I am personally guilty of this, and have many times answered ... -
Hot Plasmids and Viral Preps - May 2021
TypeBlog PostPublishedMay 18, 2021, 1:15 p.m....these therapeutics. Jesse Bloom’s lab at the Fred Hutchinson Cancer Research Center developed a pooled plasmid...identified. New from the viral service By Jason Nasse We regularly add new viral aliquots from our... -
Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells
TypeBlog PostPublishedDec. 10, 2020, 1 p.m....S, Li Y, Hough S, Tran K, Li J, Yin H, Anderson DG, Sontheimer EJ, Weng Z, Gao G, Xue W (2015) Adenovirus-Mediated... -
Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles
TypeBlog PostPublishedJune 22, 2017, 2:30 p.m....between two molecules more closely is Förster Resonance Energy Transfer, FRET. Additional opportunities...Gene Tagging in Human Cells Check Out the Michael Davidson Fluorescent Protein Collection Get Tips for Using... -
Degrading DNA with Cascade-Cas3
TypeBlog PostPublishedFeb. 11, 2025, 2:15 p.m....Yoshimi, K., Okuzaki, Y., Gee, P., Kunihiro, Y., Sonpho, E., Xu, H., Sasakawa, N., Naito, Y., Nakada, S...INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons Additional resources on Addgene.org CRISPR ... -
The AAVantages of AAV in CRISPR Screens
TypeBlog PostPublishedFeb. 4, 2025, 2:15 p.m.... A., Sousa, A. A., Tsai, S. Q., Bengtsson, N. E., Lööv, C., Ingelsson, M., Chamberlain, J. S., Corey, ... -
Chromoproteins: Colorful Proteins For Molecular Biology Experiments
TypeBlog PostPublishedFeb. 4, 2020, 2:15 p.m....Why do these organisms have chromoproteins? One reason may be that chromoproteins protect corals from ...fluorescence lifetime imaging microscopy-based Förster resonance energy transfer (FLIM-FRET) in living cells (Figure... -
3D Printing Meets CRISPR Cas9
TypeBlog PostPublishedOct. 17, 2017, 12:55 p.m....Byron Rubin, a crystallographer working with Jane Richardson, provided an initial solution to this problem...are interested in experiencing Cas9 up close and personal, visit the 3D Molecular Designs web site. Or, ... -
Tips for a 1st time AAV user (by a Rookie AAV user)
TypeBlog PostPublishedOct. 23, 2018, 11:49 a.m....consider performing PEG precipitation. Instead of sonicating the cell pellet, I lyse the cells with this AAV... Stone and Harshana de Silva Feelixge at Fred Hutchinson Cancer Center for teaching me about AAV production...