We narrowed to 705 results for: ANG

1. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

   Type

   Blog Post

   Published

   June 25, 2024, 1:15 p.m.

   ...editing efficiency such as the length of the RTT overhang, the GC content of the primer binding site, and...

2. Antibodies 101: Conventional vs Spectral Flow Cytometry

   Type

   Blog Post

   Published

   Aug. 3, 2023, 1:15 p.m.

   ...the markers in the panel. This complexity index ranges from 0-1, with a value of “0” indicating those ...

3. Transferable Skills Guide: Managing a Team

   Type

   Blog Post

   Published

   Jan. 30, 2018, 2:42 p.m.

   ...the feedback, but at least you can start making changes for the best. Positive feedback is equally as important...

4. Truncated gRNAs for Regulating Gene Expression

   Type

   Blog Post

   Published

   Jan. 10, 2017, 3:37 p.m.

   ...transcriptional regulator without making any functional changes to Cas9. For instance, when native (nuclease-competent...

5. Addgene's Cancer Collection Pages Connect the Research Community

   Type

   Blog Post

   Published

   Sept. 6, 2016, 2:30 p.m.

   ...to K‐Ras and inhibit Sos‐mediated activation." Angewandte Chemie 124.25 (2012): 6244-6247. PubMed PMID:...

6. Anatomy of a Plasmid Page at Addgene

   Type

   Blog Post

   Published

   Feb. 4, 2016, 3:30 p.m.

   ...The backbone modifications listed can refer to changes made to the original backbone by the depositing...

7. Genome-wide Screening Using CRISPR

   Type

   Blog Post

   Published

   Aug. 18, 2015, 2:30 p.m.

   ...scaled up for genome-wide screening due to the broad range of potential target sequences and ease of generating...

8. Plasmids 101: Multicistronic Vectors

   Type

   Blog Post

   Published

   Sept. 9, 2014, 8:20 p.m.

   ... entry of ribosomes during in vitro translation. Jang et al. (J Virol. 1988 Aug;62(8):2636-43.) PubMed...

9. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

   Type

   Blog Post

   Published

   June 12, 2014, 3:30 p.m.

   ...to expand the toolkit. We have included a wider range of terminators than are commonly used as we have...

10. Tips for arabidopsis transformation

    Type

    Blog Post

    Published

    Oct. 25, 2018, 1:23 p.m.

    ...Agrobacterium all over your gloves, so be sure to change gloves between constructs if you are transforming...

11. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type

    Blog Post

    Published

    Oct. 4, 2018, 12:44 p.m.

    ...sgRNA targets. CRISPRi is effective in a narrow range around the transcription start site (TSS). The Root...

12. AAVs in Retinal Gene Therapy

    Type

    Blog Post

    Published

    July 31, 2018, 12:55 p.m.

    ...engineered AAV serotypes that further expands the range of AAV properties is available. While only natural...

13. MXS Chaining

    Type

    Blog Post

    Published

    Feb. 7, 2017, 3:30 p.m.

    ...Central PMCID: PMC2574415. 3. Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA, Smith HO.Enzymatic...

14. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type

    Blog Post

    Published

    Aug. 30, 2022, 1:15 p.m.

    ...red lines), which maintains structural epitopes (orange region), but it can block epitopes hidden within...

15. Summer SciComm Series: Modes of Communication

    Type

    Blog Post

    Published

    July 1, 2022, 1:15 p.m.

    ...their science, meaning that they will heavily evangelize for the mode of communication they connect best...

16. Getting to Know Expansion Microscopy

    Type

    Blog Post

    Published

    June 20, 2023, 1:15 p.m.

    ...e73775. https://doi.org/10.7554/elife.73775  Dani A, Huang B, Bergan J, et al (2010) Superresolution Imaging...

17. An Introduction to Adenovirus

    Type

    Blog Post

    Published

    Oct. 3, 2023, 1:15 p.m.

    ... very diverse family of viruses with broad host range and tissue tropism (Borkenhagen et al., 2019). AdVs...

18. Transferable Skills Guide: Identifying Your Transferable Skills

    Type

    Blog Post

    Published

    Jan. 11, 2018, 3:29 p.m.

    ... you’re more of a leader than you thought! When change happens at your organization, when your boss needs...

19. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type

    Blog Post

    Published

    Aug. 9, 2016, 2:30 p.m.

    ...as a linear polymer of A,T,G, and C nucleotides arranged in a double helix. People are wrong. In reality...

20. Negotiating Work and Life: How to Find the Joy

    Type

    Blog Post

    Published

    March 20, 2014, 3:04 p.m.

    ... your work. Nobody wins, and you need to make a change. #2 Thou shalt sometimes put work ahead of life...