We narrowed to 897 results for: ANT

...are some additional features. The first and most imporant is that, upstream of the Cbh promoter, this plasmid...GFP-tagged and un-tagged versions of SpCas9 and further variants replace SpCas9 with SpCas9n (nickase), which nicks...homology directed repair. You can find all of these variants, as well as links to the Zhang Lab's Nature Protocols...

...focus on an aspect of communication especially important for a manager – getting feedback from the team... This can take practice and training. This is important because it creates a culture of engagement in ...  Seeking feedback frequently One of the most important paths of information flow is the one that brings...

...competition featuring a selected ingredient. Anyone who wants to participate brings in a dish that features that... for our website. Free lunch is provided for participants during the Hack Days, and we’ve seen great success...easy as possible. However, I think it’s just as important to take a break from work to share time with the...

...activities. Working with proteins requires a substantial amount of properly folded, relatively pure protein...al. "The pCri system: a vector collection for recombinant protein expression and purification." PloS one..., Zachary T. Britton, and Anne S. Robinson. "Recombinant protein expression and purification: a comprehensive...

...defend the thesis, etc. Photography is critically important in many fields of biology and I believe it could... use of x-rays. His haunting images have been enchanting people for years and have gathered much acclaim...method to explore the inner structures of delicate plants and other organisms without destroying the original...

...It was immediately clear that Addgene wanted to ensure applicants understood what the expectations were... with the different types of issues that were important to this function. With the welcoming environment...

...studying COVID-19. “Open scientific sharing is important for creating research synergies and accelerating...believe that by coming together, we can create a significant impact on both clinical care and public health..., scientific sharing will remain an extremely important part of the future of research. To find other ...

... from rare diseases by connecting patients to important resources and promoting research for rare disease...disease. This year, Addgene will be providing plasmid grants worth $2,000 each to two labs through the BeHEARD...physicians and scientists.  Although the form of malignant mesothelioma that affects the lining of the lungs...

...Conference at Boston University last week.  This fantastic city-wide effort was led by two amazing postdocs...practices of indirect costs.  The peer review and grant awards systems need to be radically overhauled.  ...alternate training needs to be provided to take advantage of all the possible science careers. She advocated...

...comparing the CRISPR/Cas9 system to Norton Antivirus. Antivirus programs work by taking info from the code...Prize. What did Dr. Doudna think was the most important part of their work? First, the discovery of a ...

...and shared by others to develop an anti-tumour therapy using antibodies targeting dendritic cells. These... over 250 scientists in Europe. Despite these fantastic numbers, a lot of European scientists and institutions...

...– a liver or brain, say, or maybe a tumor. They wanted to find something better. Transparency window That...allowing higher resolution in samples containing substantial autofluorescence background.” Just last month...when in doubt, read the methods sections of the relevant papers, and then read them again. Most of the ...

...easily manipulate specific genes. But what if you want to study genes all across the genome? Two new human... and tricks Wang offers this tip: “It's quite important, at every step of the process, that you are not...not losing library representation.” It’s also important that you infect enough target cells. What’s “enough...

... zones, it’s important that everyone can access data and drafts of manuscripts and grant applications ...directions of the project. The state of research is constantly shifting, and long-distance collaborations are...

...popularity of single-use items, plastic is a substantial portion of lab waste. Despite the fact that researchers...challenge you to take to action! These days, it’s important for individuals, companies, and universities to...to work on this issue. Be a champion for this important cause! To get more lab sustainability tips, check...

...left not really knowing what I wanted to do, but knowing that I wanted to leave the bench.” Her path to...called “Teaching Your Research.” This course has participants take their research from the bench to the classroom...

... organisms: mammalian cells, yeast, drosophila, plants, worms, zebrafish, frogs, some bacteria; but not...has maximum activity from 50-70 °C. The key advantage of GeoCas9 is that it functions at temperatures...yet? Let us know in the comments below. And if you want to give GeoCas9 a try, as always, the plasmids are...

...worldwide and the presentation made it clear how important it is for us to improve our resources for this... meet hundreds of the over 11,000 conference registrants, including scientists from six continents who...presentation at the CRISPR screens symposia outlined the fantastic work this Broad Institute lab is doing creating...

...invading phages. Rising to the challenge - Evolution of anti-CRISPR genes in phages The CRISPR/Cas adaptive immune...CRISPR/Cas. Earlier this year, expression of 1 of 5 anti-CRISPR genes were found to inactivate the Type I-F... the presence of a variety of undiscovered viral anti-CRISPR genes. These data highlight an interesting...

...to identify specific cells — as well as their descendants. This makes them useful for lineage tracing, ...BioRender.com. The CellTag libraries from the Samantha Morris lab use a combinatorial barcoding approach...protein, teaching us more about viral evolution and antibody binding. Figure 3: Schematic of phage display...