We narrowed to 904 results for: ANT

...colony to DNA extraction and quantification so that you can focus on what you want to do with your plasmid ... grades of DNA  Read about the various ways to quantify DNA Resources on Addgene.org Learn about our...

...peek in case you missed them. July 2016 was a fantastic month for the Addgene blog. We achieved roughly...editing when compared to the most commonly used Cas9 variant, SpCas9. These properties, combined with other ...in the post, may make Cpf1 the preferred CRISPR variant going forward.   Special Delivery Fluorophore Targeting...

...been working with Seeding Labs to provide plasmid grants to researchers in developing countries. Today we...announce that we’ve selected our first two plasmid grant awardees: Drs Louis Bengyella and Kwabena O. Duedu...excited to discover what new and creative ideas applicants will propose for plasmids from the Addgene community...

...into next year we’d like to know what topics you want to learn more about. Our post on “Making Your Own...Finally, we are always looking for guest bloggers who want to share their research with the community, so email...Mentoring Support By Joanne Kamens Plasmids 101: Antibiotic Resistance Genes By Marcy Patrick Plasmids...

...fresh new friend: Antibody of the Day!  Figure 4: The plasmid, viral prep, and antibody of the day While...others may be less obvious — but every change is meant to make your experience on our website better so...

...programmable DNA-binding domains" publication.  If you want to see this new feature in action, click here to...plasmid of interest, which may help you identify relevant protocols and experimental data Find other related... an article that used a plasmid from Addgene, we want to hear about it so we can link to it in the "Articles...

...Product Management “I knew very early on that I wanted to be an artist/designer. But there were a few ...Campbell, and read about his "follow your bliss' mantra and never forgot it. Usually when I see inspiring...Designer On the job search “Always go for the job you want, even if you don’t think you’re qualified. The worst...

...are some additional features. The first and most imporant is that, upstream of the Cbh promoter, this plasmid...GFP-tagged and un-tagged versions of SpCas9 and further variants replace SpCas9 with SpCas9n (nickase), which nicks...homology directed repair. You can find all of these variants, as well as links to the Zhang Lab's Nature Protocols...

...focus on an aspect of communication especially important for a manager – getting feedback from the team... This can take practice and training. This is important because it creates a culture of engagement in ...  Seeking feedback frequently One of the most important paths of information flow is the one that brings...

...competition featuring a selected ingredient. Anyone who wants to participate brings in a dish that features that... for our website. Free lunch is provided for participants during the Hack Days, and we’ve seen great success...easy as possible. However, I think it’s just as important to take a break from work to share time with the...

...activities. Working with proteins requires a substantial amount of properly folded, relatively pure protein...al. "The pCri system: a vector collection for recombinant protein expression and purification." PloS one..., Zachary T. Britton, and Anne S. Robinson. "Recombinant protein expression and purification: a comprehensive...

...defend the thesis, etc. Photography is critically important in many fields of biology and I believe it could... use of x-rays. His haunting images have been enchanting people for years and have gathered much acclaim...method to explore the inner structures of delicate plants and other organisms without destroying the original...

...It was immediately clear that Addgene wanted to ensure applicants understood what the expectations were... with the different types of issues that were important to this function. With the welcoming environment...

...studying COVID-19. “Open scientific sharing is important for creating research synergies and accelerating...believe that by coming together, we can create a significant impact on both clinical care and public health..., scientific sharing will remain an extremely important part of the future of research. To find other ...

... from rare diseases by connecting patients to important resources and promoting research for rare disease...disease. This year, Addgene will be providing plasmid grants worth $2,000 each to two labs through the BeHEARD...physicians and scientists.  Although the form of malignant mesothelioma that affects the lining of the lungs...

...Conference at Boston University last week.  This fantastic city-wide effort was led by two amazing postdocs...practices of indirect costs.  The peer review and grant awards systems need to be radically overhauled.  ...alternate training needs to be provided to take advantage of all the possible science careers. She advocated...

...comparing the CRISPR/Cas9 system to Norton Antivirus. Antivirus programs work by taking info from the code...Prize. What did Dr. Doudna think was the most important part of their work? First, the discovery of a ...

...and shared by others to develop an anti-tumour therapy using antibodies targeting dendritic cells. These... over 250 scientists in Europe. Despite these fantastic numbers, a lot of European scientists and institutions...

...– a liver or brain, say, or maybe a tumor. They wanted to find something better. Transparency window That...allowing higher resolution in samples containing substantial autofluorescence background.” Just last month...when in doubt, read the methods sections of the relevant papers, and then read them again. Most of the ...

...easily manipulate specific genes. But what if you want to study genes all across the genome? Two new human... and tricks Wang offers this tip: “It's quite important, at every step of the process, that you are not...not losing library representation.” It’s also important that you infect enough target cells. What’s “enough...