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We narrowed to 898 results for: CAN

Showing: 661 - 680 of 898 results

  1. 10 Ways to Share Your Science!

    Type
    Blog Post
    Published
    May 13, 2014, 8:34 p.m.
    ...time to hone your communication skills. So, where can a graduate student or post-doc start? The good news...your science with the community. Some of them you can do today! 10 ways to share your science 1)   If you...consider how those stories are being told. Twitter can be a good way to follow news about particular subjects...

  2. Find and Share AAV Data with Addgene's New AAV Data Hub

    Type
    Blog Post
    Published
    Oct. 8, 2019, 12:33 p.m.
    ...Every entry has an accompanying image so that you can see for yourself how the AAV performed in that experimental...we hope to mine the data to find patterns that we can share with you about promoters, serotypes, expression...

  3. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    Published
    March 28, 2024, 1:15 p.m.
    ...interact with extracellular ligands, their ectodomains can extend and open, binding ligands with high affinity...function. With the right research reagents, researchers can measure this conformational change to investigate... cellular processes.   Figure 1: Integrins can shape into three conformations: (A) bent-closed, ...its open state, activating the receptor. Ligands can bind to integrins in their closed states (A) and ...laboratory techniques related to antibody discovery. You can find cell surface receptor biology talks on the IPI...kidney development. Despite their ubiquity and significance, these receptors have proved challenging to ...

  4. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    Published
    Oct. 24, 2017, 1:54 p.m.
    ... as often as they can. Karissa Milbury is currently a PhD candidate at the BC Cancer Agency and University... April 22, 2017. While experiments (and editors) can move slowly, news about possible broad changes to...also studies energy markets in his spare time. You can follow him on twitter at @thompn4. New York City,...stars and the instruments we use to study them. You can follow her at alexandrazgreenbaum.wordpress.com or...supporters. I'm raising some little scientists (as you can see in the picture) and this march proved to be the...Within the Sciences Learn How Changing Labor Laws Can Effect Postdoc Wages ... breast cancer at the Goodman Cancer Research Centre at McGill University in Montreal, Canada.  Outside...

  5. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ...Sometimes the PCR products can look smeared, so having an undigested control can help distinguish between.... Cas9 delivery Overview: Before any editing can begin we need our A549 cells to express Cas9. In ...Addgene viruses are provided at a specific titer, you can better plan your transductions and use a range of...well dish. My protocol with many additional tips can be found here. Figure 3: A surprise for the author...gene. I used existing BRAF gRNA viruses, but you can find a list of validated gRNA plasmids on our website... implicated in several human cancers) in A549 cells (a human lung cancer cell line), armed only with viruses... CRISPR, I was able to knockout BRAF in a human cancer cell line in about 1 month, which I’d say is pretty...

  6. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ...journal club. This can be a challengingly vague metric but there are a few things you can point to. Does ...advance notice for the first meeting so that people can set aside time in their schedules and read the paper...should read the paper ahead of time!). This notice can be shortened as the meeting settles in and people...

  7. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...anti-SARS-CoV-2 antibodies (Fig 1B). This approach can augment viral surveillance by enabling rapid assessment...particles (MPyV VLPs ). This artificial nanoparticle can protect proteins from unfavorable interactions in...more D-glucaric acid, suggesting that MPyV system can improve metabolic pathway yields by encapsulating...the population.  Because indel generation by Cas9 can occur at a steady rate, scientists found a way to...Mapping antibody escape in SARS-CoV-2 by mutational scanning By Seth Kasowitz As multiple antibody-based vaccines...therapeutics. Jesse Bloom’s lab at the Fred Hutchinson Cancer Research Center developed a pooled plasmid library... the antibody toolbox, they screened antibody candidates using a synthetic human scFV library and characterized...

  8. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    Published
    Sept. 7, 2017, 12:17 p.m.
    ...resource-consumptive, since only wells with one cell can give rise to single clones, and thus many plates ...truncated pipet tips. Indeed, an experienced operator can pick 48 clones from a 10 cm dish in 15- 20 minutes... a fluorescent protein), fluorescence microscopy can theoretically be used to select specific wells from...peptide maturation in neural and endocrine cells. She can be emailed at [email protected].   Additional... secondary biological screens. For example, for cancer researchers, if growth properties in agarose are...

  9. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...proved to be quite easy to achieve…” - but I think we can all agree that it sounds less than ideal.  Unexpected...amenable to protein engineering. As a result, you can now find Myc1-9E10 in a variety of isotypes like ...refinement of this trusty tool doesn’t end there: you can also find anti-Myc [9E10] Fab fragments and single-chain...9E10’s accessibility and the variety of formats you can find it in likely mean it will remain an important... the N-terminal half of the antigen differs significantly between human and the other species, suggesting...

  11. DIY DNA Ladders from Penn State University

    Type
    Blog Post
    Published
    July 14, 2017, 2:30 p.m.
    ...Two plasmids that can be used to make inexpensive 100 bp or 1 kb DNA molecular weight ladders were recently...interested in making your own DNA ladder? You can find the Tan Lab’s DNA ladder prep protocol here and ...worth of ladder. The Penn State ladders are significantly cheaper. When using a homemade plasmid prep ...increases the costs to $20-30 per ladder: still significantly cheaper than commercial ladders. Are you interested...

  12. Activate Addgene's "Find Plasmids" Badge on Pubmed Abstracts

    Type
    Blog Post
    Published
    May 12, 2017, 12:58 p.m.
    ...handy tool for you.   With a few quick steps, you can use PubMed to see which articles contain plasmids...sure to check out the other “LinkOut” badges you can add and use your new PubMed account to set-up emails...

  13. Antibody Data Repositories and Search Engines

    Type
    Blog Post
    Published
    Oct. 31, 2024, 1:15 p.m.
    ...looking to find one. Luckily, there are websites that can help: Antibody data repositories, which share validation... that you use? Let us know in the comments so we can add it to the table! And if you're interested in ...Laboratory Cancer Any Data repository From the Frederick National Library for Cancer Research ...

  14. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    Published
    April 19, 2017, 3:25 p.m.
    ...challenges in NGS by developing technologies that can help unlock the potential of modern sequencing instruments...because the yield or quality of individual libraries can be influenced by the quantity or quality of input...normalization steps, measured in time and consumables, can be daunting for large numbers of samples. As mentioned...

  15. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    Published
    Sept. 12, 2019, 1 p.m.
    ...AAV we have released in the last few months. You can also browse our entire AAV inventory. Our new AAVs... AAV2, AAV5, AAV8, AAV9, and AAV Retrograde. You can also request the specific serotypes you need instead... exhibits the brightest resting fluorescence and can be used for imaging small neuronal processes (dendrites...

  16. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    Published
    Jan. 24, 2019, 2:24 p.m.
    ... Tetbow for neuronal labeling To use Tetbow, you can introduce the four Tetbow plasmids (Addgene #104102...2014) (Addgene #104106 - #104108) (Figure 3) that can be introduced using in utero electroporation. Then...website. We update the site regularly and hope Tetbow can help you in your research.     To see Tetbow in ...

  17. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    Published
    Dec. 20, 2018, 1:15 p.m.
    ...things we can navel-gaze over from the last couple of weeks, including whether or not scientists can recognize...model of public involvement, research, and consensus can happen. We just have to look to the United Kingdom...

  18. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    Published
    March 24, 2016, 2:30 p.m.
    ...repaired by homology directed repair (HDR) pathways can modify the sequence of a gene (thus altering its ...revealed some of the biophysical parameters that can influence the HDR/NHEJ decision. Our conclusions ... the sequences of our validated editing reagents can be found on our plasmid pages. We encourage any labs...

  19. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    Published
    Aug. 25, 2015, 2:30 p.m.
    ...functional prototype to gain any real traction.  This can carry a heavy price tag, especially if you're working...focused on empowering early-stage scientists. You can think of miLab as a sort of talent agency for scientists... of the potential contacts your personal network can offer, you are really on your own to build a strategic...majority of these meetings acted as a platform for significant company advancement. Be relentless The world ...

  20. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ...discovered in the HIV protein Tat. Although CPP fusions can translocate a cell membrane, the presence of the ... took up both Cas9 protein and a gRNA (yes, iTOP can also be used for RNA transduction) targeting a gene...Na+ hypertonicity are key elements of iTOP. iTOP can be used to transduce Cas9 and a gRNA, which subsequently...
Showing: 661 - 680 of 898 results