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TypeBlog PostPublishedDec. 5, 2014, 2:54 p.m....online accessibility. It is now about as easy to find a paper in a lesser-known journal as it is in a ...-tier publication. Accessibility is surely also behind the increase in citations for older publications...publications. Ventures like Impact Story seek to find ways to capture scholarly work that exists outside...
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Measuring Kinase Activity at the Single-Cell Level with Kinase Translocation Reporters (KTRs)
TypeBlog PostPublishedJuly 26, 2018, 12:46 p.m.... Additional Resources on Addgene.org Find FRET plasmids Find fluorescent biosensors ...Nature protocols, 13 1, 155-169. PMID: 29266096. Find the KTR plasmids here! Additional Resources on the... -
Science Communication for Everyone
TypeBlog PostPublishedApril 16, 2019, 1:05 p.m....world and help them share their lab experiences, findings, and experiments as articles written for general...Google search. Be careful about hidden connotations behind a word. Non-scientists interpret terms like manipulations...BioTechniques. Additional resources on the Addgene blog Find all of our science communication posts Sign up to... -
Top Requested AAV of 2017: pmSyn1-EBFP-CRE
TypeBlog PostPublishedJan. 17, 2018, 2:57 p.m....therefore mCherry will only be expressed in neurons. Indeed, this AAV provides you with a means to specifically...coming months so be sure to check back often! To find more viral vectors available through Addgene, be...Viral Service Page Browse Additional Viral Vectors Find Viral Vector Protocols ... -
Reflections and Looking Ahead
TypeBlog PostPublishedFeb. 25, 2025, 2:15 p.m....resources. As we step into 2025, these milestones remind us of the extraordinary impact that happens when...also redesigned our website to make it easier to find what you need and to bring it in line with current...and reinventing our search experience so you can find exactly what you need, faster. We’re also building... -
Scientist Networking: What is an Informational Interview?
TypeBlog PostPublishedJuly 1, 2014, 3:42 p.m....available once they have a graduate degree in science. I find it ironic that we do all of our training as scientists... list and take notes if that is helpful. Keep in mind that this is a good time to ask personal questions...having to talk with strangers. Be bold in asking all kinds of people to connect with you and to share their... -
Easing the Protein Purification Process with pCri
TypeBlog PostPublishedJune 19, 2015, 3:08 p.m.... expression vector and expression system. If you find that your protein might not fold well, you should...cited below. pCri plasmids are available either individually or as a complete kit from Addgene. References...Plasmids 101: Protein tags Resources on Addgene.org Find Additional Cloning Kits ... -
A Better Way to Get Customer Support: The Help Center
TypeBlog PostPublishedDec. 10, 2015, 3:30 p.m....search through the entire FAQ collection. You’ll find topics ranging from depositing your lab’s materials...the support you need. If for some reason you can’t find the answer that you’re looking for, or you just ...Pages Resources at Addgene Visit the Help Center Find Plasmids for Your Research Become a Member of the... -
Mapping the 4D nucleome with CRISPR/Cas9
TypeBlog PostPublishedAug. 11, 2015, 2:30 p.m....10.1073/pnas.1420024112. Epub 2015 Feb 23. Pubmed. Find the plasmids from this publication at Addgene. ...1479-91. doi: 10.1016/j.cell.2013.12.001. Pubmed. Find the plasmids from this publication at Addgene. ...10.1016/j.cell.2014.09.039. Epub 2014 Oct 9. Pubmed. Find the plasmids from this publication at Addgene. ... -
6 Steps to Submitting a Resume That Gets Seen
TypeBlog PostPublishedJuly 9, 2015, 2:30 p.m....chance you have of finding a beneficial connection. LinkedIn is one great way to find an appropriate liaison...application. 3) Read job descriptions carefully Be open minded and be bold. You don’t have to have all of the ... -
New CRISPR Web Resources and #12DaysofCRISPR Recap
TypeBlog PostPublishedDec. 12, 2017, 3:13 p.m....(and, of course, find plasmids that will be useful to you.) With these goals in mind, we set out to update... plasmid class covered on that page. To help you find popular plasmids, we’ve also added four new plasmid... -
Chemogenetics vs. Optogenetics: Which Method Should I Choose?
TypeBlog PostPublishedJune 11, 2020, 1:34 p.m.... commonly through viral delivery by AAV. You can find AAV tools for optogenetics and chemogenetics on ...controlled Unique advantages Mapping the brainDissecting circuits Studying GPCR pathways Acknowledgements...available at Addgene Visit our viral vectors guide Find our chemogenetics and optogenetics science guides... -
Plasmids 101: What is a plasmid?
TypeBlog PostPublishedApril 2, 2020, 6:17 p.m....small circular pieces of DNA that replicate independently from the host's chromosomal DNA. They are mainly... markers for use in other cell types. Primer Binding Site A short single-stranded DNA sequence used ...cloning methods (restriction enzyme, ligation independent, Gateway, Gibson, etc). The cloning method is... -
Addgene's a Nonprofit? Nonprofit Awareness Day 2017
TypeBlog PostPublishedJune 5, 2017, 3:19 p.m....organization what do you think of? Maybe the term brings to mind a social service organization like the Red Cross...organization cannot distribute profits to private individuals through stock or investor pay outs. Addgene has...relationship with our community members. We also find that, as a nonprofit, we attract partnerships with... -
CRISPR 101: Drug discovery and CRISPR
TypeBlog PostPublishedMay 4, 2023, 1 p.m....disease progression or onset. These hits can reveal individual genes or sometimes even genetic pathways that...of possibilities greatly increases the odds of finding a quality drug downstream. In some cases, there...generate patient mutations and then test both individual and combinatorial drugs on for effectiveness ... -
Bricking Science: Portraying Scientific Reality Through LEGO
TypeBlog PostPublishedNov. 29, 2016, 3:30 p.m....experience this very human side to scientific research. Find Protocols Lab Tips to Keep Your Experiments Running...protocols, guidelines, and standards. Bricking science reminded us of our inner children who have no preconceived...to Share Your Science Resources on Addgene.org Find Protocols and Protocol Videos Check out LeGO plasmids... -
The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place
TypeBlog PostPublishedJan. 21, 2020, 2:30 p.m.... and links provided, in addition to a list of individual screens curated for the publication. Clicking...blog post about genome-wide screening using CRISPR Find all of our CRISPR pooled library blog posts Check... guide Learn more about plasmid pooled libraries Find CRISPR plasmids for your research ... -
History of CRISPR Cas - A tale of survival and evolution
TypeBlog PostPublishedNov. 25, 2013, 10:12 p.m.... the CRISPR (clustered regularly interspaced palindromic repeats) Cas (CRISPR associated genes) system...adjacent motif (PAM). Once the CRISPR/Cas complex binds to the foreign DNA, a cut is made to destroy the... CRISPR/Cas, check out Addgene’s CRISPR Guide or find CRISPR/Cas plasmids for your research. Do you have... -
Identifying Sequence Elements with SnapGene's Feature Database
TypeBlog PostPublishedDec. 21, 2017, 2:06 p.m....tolerates occasional mismatches or indels. Empirical tests indicated that a reasonable rule is to require...with SnapGene Server is useful because it helps us find which common features need to be added to the database... -
Science Careers: Unruly Interests Feed Many Paths
TypeBlog PostPublishedSept. 26, 2017, 12:12 p.m.... path at a time, the variety of paths in life is mind-boggling. Unlike a mountain – with many routes up...stochasticity of molecular interactions and embedding individual diversity to get from gamete to a visibly complex... to Career Blog Posts Resources at Addgene.org Find Job Openings at Addgene ...