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Addgene
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  1. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...bbdone with our bbduk process.    At first, to make sure we understood the concept of channels and implemented...

  2. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ...work stalls. We become so fixated with the idea of making something the best it can be that it seems impossible...

  3. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ... and high specificity of anti-epitope antibodies make this approach appealing, but the disadvantages must...

  4. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ...retrograde direction from the synapse to the cell body, making it a great tool to study neuronal connectivity....

  5. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    Published
    April 11, 2024, 1:15 p.m.
    ...//doi.org/10.1111/1574-6968.12241  Keen, N. T., Tamaki, S., Kobayashi, D., & Trollinger, D. (1988). Improved...

  6. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...often looks like the graph on the left, it might make more sense once you realize events are plotted on...

  7. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ... than engineering an entirely new protein. This makes it much cheaper to use Cas9 activators. This larger...

  8. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...gRNA targets in that region. The CRISPR Software Matchmaker can be used to determine the pros and cons of...

  9. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ... generally ensure cell line identity and purity making it easier for comparable studies to be performed...

  11. Plasmids 101: Control Plasmids

    Type
    Blog Post
    Published
    April 9, 2015, 3:29 p.m.
    ...reproducibility controls". Biological replicates are what makes up your sample size (aka your n value)" and should...

  13. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ...exposed side chain that can be easily manipulated to make reactive species without affecting the avidin binding...

  16. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    Published
    June 27, 2024, 1:15 p.m.
    ...gating uses the same color for different markers, making it easy to gate on the negative cell population...

  19. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    .... Li, K et al. PLoS ONE 9(8); (2014). PubMed. Making designer mutants in model organisms. Peng, Y. et...

  20. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ...iGECI and NIR-GECO2G, and its small size (~1500 bp) makes iGECInano suitable for AAV packaging.    Find iGECInano...
Showing: 661 - 680 of 698 results