We narrowed to 861 results for: REN;
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TypeBlog PostPublishedNov. 10, 2015, 3:30 p.m.... like FLAG, HA, and Myc can adopt a number of different conformations and thus potentially interact with...our older epitope tags - only time will tell! References 1. Georgieva, Maya V., et al. "Inntags: small...
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Stem Cell Models for Disease & Open Science: Interview with Darrell Kotton
TypeBlog PostPublishedJuly 7, 2017, 1:32 p.m....Research currently under way in the Kotton Lab. One of Darrell's favorite projects - differentiating surfactant... -
What's New in CRISPR - Spring 2019
TypeBlog PostPublishedMarch 21, 2019, 12:47 p.m....hisashii, BhCas12b that is functional at 37 °C but preferentially nicks the non-target DNA strand. The lab then...approach, the Jennifer Doudna lab identified three different Cas12a inhibitors. Some of these anti-Cas12a proteins... -
Why Do I Need an MTA Anyway?
TypeBlog PostPublishedMay 1, 2014, 8:23 p.m....or choose to auto-approve MTAs. Whatever your preference, we are here to make it fast and easy. Fast....org or +1 617-225-9000, or check out our MTA reference pages. So go ahead and use Addgene plasmids ... -
Reflections and Looking Ahead
TypeBlog PostPublishedFeb. 25, 2025, 2:15 p.m....find what you need and to bring it in line with current Web Content Accessibility Guidelines (WCAG). These...developments? Subscribe to the Addgene newsletter. References Ayoubi, R., Ryan, J., Gonzalez Bolivar, S., Alende... -
Bringing Sustainable Practices to the Lab: Innovations
TypeBlog PostPublishedMarch 7, 2019, 2:39 p.m.... emphasizes Accountability, Consistency, and Transparency. This label scores products by their environmental...pipette tips in a high-throughput and efficient way? Grenova, for example, offers automated, benchtop, high ... -
Phage Directory: From Phage Therapy to a Repository of Phage Information
TypeBlog PostPublishedJan. 10, 2019, 2:38 p.m....test. These includes labs with a large number of different phages or with phages targeting organisms closely...prepare phages for clinical use. There are also different regulations from country to country when it comes... -
Scientific Sharing in the Time of COVID-19: Databases and Resources
TypeBlog PostPublishedMay 12, 2020, 1:15 p.m....strives to match up scientists across fields with different technical knowledge that could help fight COVID... both clinical care and public health for this current COVID-19 pandemic, and outbreaks that have yet ... -
Who Gives a Tweet? 9 Facts About Scientists on Twitter
TypeBlog PostPublishedJune 26, 2017, 1:31 p.m....never clicked for me. Recently my interest was renewed in part due to FOMO but mostly because of this.... And don’t forget to follow us @Addgene. References Ke, Q., Ahn, Y., & Sugimoto, C. R. (2017). A ... -
What's New in CRISPR - March 2020
TypeBlog PostPublishedMarch 10, 2020, 1:15 p.m....controls nuclear localization (LINuS). Blue light renders the NLS accessible and the complex enters the nucleus...cycle, asymmetric cell division, and cellular differentiation, but it takes several weeks to delete or deplete... -
Cre-ating New Methods for Site-specific Recombination in Drosophila
TypeBlog PostPublishedMay 12, 2015, 1:32 p.m....the efficiency of Cre recombination varies for different constructs and cell types. Second, Cre may induce...enhance genome editing capabilities in Drosophila. References: Multiple new site-specific recombinases for ... -
Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner
TypeBlog PostPublishedSept. 15, 2014, 6:05 p.m....Charpentier. I was joined by Addgene Scientist Matt Ferenc (one of our resident CRISPR experts) at the 2014...system Drs. Dounda and Charpentier met at a 2011 conference and decided to collaborate on what was a “curiosity... -
Some Like it Hot: Thermostable GeoCas9
TypeBlog PostPublishedSept. 14, 2017, 12:40 p.m.... just find one that naturally occurs? This is different than the engineered approach used to make eSpCas9...are available from Addgene. Happy CRISPRing! References Harrington, Lucas, et al. "A thermostable Cas9... -
SunTag and Fluorescent Imaging
TypeBlog PostPublishedMarch 28, 2017, 2:30 p.m....SunTag? Tanenbaum et al. performed a variety of different experiments in their paper, and so can you! In...we’ll save that application for another day! References 1. Tanenbaum, Marvin E., et al. “A protein-tagging... -
Build Your CRISPR Vocabulary
TypeBlog PostPublishedJuly 2, 2024, 1:15 p.m....nontarget strand. Protein Cas: Different CRISPR systems involve different proteins, but many of them are... -
We're Updating Our Hold for Publication Policy
TypeBlog PostPublishedJune 24, 2025, 1:15 p.m....costly for us to process and store materials that aren’t distributed or for our teams to keep searching,...materials are both excellent ways to provide transparency, support reproducibility, and accelerate science... -
Bricking Science: Portraying Scientific Reality Through LEGO
TypeBlog PostPublishedNov. 29, 2016, 3:30 p.m....standards. Bricking science reminded us of our inner children who have no preconceived obstacles, optimistically...potential, and who sometimes wish they could live differently than we do day to day. We discovered that it ... -
Plasmids 101: Positive and Negative Selection for Plasmid Cloning
TypeBlog PostPublishedAug. 22, 2019, 12:43 p.m....includes an antibiotic resistance cassette and you aren’t introducing an additional antibiotic resistance...plasmids at Addgene Read our molecular biology reference Watch molecular biology protocol videos ... -
CRISPR 101: Cas9 Nickase Design and Homology Directed Repair
TypeBlog PostPublishedMarch 15, 2018, 12:59 p.m....comparison to each other? The gRNAs must target different strands to create a DSB, but this can be accomplished...Shuqi Yan designed experiments to test nickase preferences using HEK293 cells. They started by comparing... -
New CRISPR Web Resources and #12DaysofCRISPR Recap
TypeBlog PostPublishedDec. 12, 2017, 3:13 p.m....some our most popular CRISPR blogs to keep them current. Here are some of the changes we’ve made: New CRISPR...already has five blue flame plasmids covering three different epigenetic effectors. The diversity of tools is...