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We narrowed to 863 results for: STI

Showing: 661 - 680 of 863 results
  1. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    Published
    Jan. 22, 2019, 2:41 p.m.
    ...of red to green fluorescence allows for clear distinction between vacuolar/lysosomal and cytoplasmic Rosella...acting as an internal control. Autophagic flux is estimated by the GFP/RFP ratio: a decrease in GFP fluorescence...
  2. CRISPR Challenges: Standardization and Homology Directed Repair

    Type
    Blog Post
    Published
    Feb. 14, 2018, 2:36 p.m.
    ... show that this basic concept of gRNA design is still challenging for researchers using CRISPR, and that...you’d like to avoid editing the genome and just stick to RNA? Cas13 may be able to help you do that! How...
  3. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    Published
    Jan. 25, 2018, 2:09 p.m.
    ...Backstrom, a biochemist in the Vanderbilt Eye Institute and Tonia Rex's lab. A common strategy to determine...Backstrom, Ph.D is a biochemist in the Vanderbilt Eye Institute and is particularly interested in cell signaling...
  4. Identifying Sequence Elements with SnapGene's Feature Database

    Type
    Blog Post
    Published
    Dec. 21, 2017, 2:06 p.m.
    ...But what makes software good? Fortunately, that question has been thoroughly answered by experts in human-computer...engaging with users of the software. We welcome suggestions about common features from anyone who uses SnapGene...
  5. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    Published
    Aug. 21, 2014, 3:15 p.m.
    ...researchers. For many years I struggled with the questions of “Will I leave academia?” and "What type of ...the Harvard Business Review or some Chartered Institute of Marketing reviews, I would have struggled. ...
  6. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ...have been able to study the effects of various stimuli on global translation, but a visual technique to... determine if proteins are targeted to their destination co- or post-translationally. TRICK: Visualizing...
  7. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    Published
    April 4, 2023, 1 p.m.
    ...either fission or budding, which is the primary distinction between two of the most frequently used yeast...highly efficient at protein secretion, making harvesting recombinant proteins a cinch. This is because...
  8. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    Published
    Aug. 23, 2022, 1:15 p.m.
    ...misinformation about sexual and reproductive health, as the stigma around sexual and reproductive health issues creates...never forgot about all the anxious, whispered questions from friends and classmates back in high school...
  9. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    Published
    March 14, 2019, 1:54 p.m.
    ...2014), but scientists have yet to decipher the mechanistic aspects behind these improvements. What have..."Enhanced liver gene transfer and evasion of preexisting humoral immunity with exosome-enveloped AAV vectors...
  10. 5 Great Apps for Lab Life

    Type
    Blog Post
    Published
    Sept. 22, 2016, 2:30 p.m.
    ...technique, I try to look for a resource to help me. I’m still waiting for a mass spectrometry app. I digress. ...one convenient resource. I can safely say that I still have the single and triple abbreviations of the ...
  11. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    Published
    Oct. 9, 2014, 3 p.m.
    ...spectra differ sufficiently for them to be imaged in distinct fluorescent channels on the microscope. With the... to choose fluorescent proteins that can be distinguished from one another and that can be imaged with...
  12. Advice for Scientists Starting a Lab

    Type
    Blog Post
    Published
    April 29, 2014, 1:43 p.m.
    ...available from your institution, your department, and online. The Howard Hughes Medical Institute (HHMI) offers...
  13. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    Published
    July 22, 2025, 1:15 p.m.
    ...gold standard in gene expression analysis to investigate the effect of regulatory elements — such as promoters...interaction occurs, the luciferase emits its characteristic light. However, if a protein interaction brings...
  14. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    Published
    Aug. 6, 2024, 1:15 p.m.
    ...prototypical gamma-retrovirus.    Frequently asked questions about γ-retroviral vectors How do engineered γ-retroviruses...post‐transcriptional regulatory element; sequence that stimulates the expression of transgenes via increased nuclear...
  15. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    Published
    June 6, 2024, 1:50 p.m.
    ...application. These controls will allow you to distinguish real results from background noise or nonspecific...positive and negative cells. This can be achieved by adjusting your voltage using your single color controls ...
  16. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ... a jigsaw puzzle, the oligos were designed with sticky ends that only anneal together in one direction...plasmids available from Addgene. If you have any questions or thoughts about Cpf1 multiplexing, leave them...
Showing: 661 - 680 of 863 results