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We narrowed to 886 results for: URE

Showing: 661 - 680 of 886 results

  1. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    Published
    May 9, 2019, 1:15 p.m.
    ...successfully reprogram. Unfortunately scRNA-seq only captures the gene expression of cells at a single time ...analysis and the remaining cells were maintained in culture. iEP cell physiology and reprogramming efficiency... lineage and identity in direct reprogramming." Nature 564.7735 (2018): 219. PubMed PMID: 30518857. Guo...

  2. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    Published
    Sept. 15, 2015, 2:30 p.m.
    ...stressors, including emotional stress or changes in temperature.  “The disease was first described in 1971,” ..., it is still sometimes wrongly diagnosed as a seizure disorder.” In 2012, researchers identified the ...Thailand will receive much better health care in the future based on our studies.” Thank you to all the BeHeard...

  3. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    Published
    Aug. 25, 2015, 2:30 p.m.
    ...that we aren’t still pitching for feedback.  Your venture should continuously evolve and respond to feedback...number of experts, including Harvard/MIT faculty, venture capital and angel investors, and business development...idea has potential to make a difference, then make sure you’re able to answer every possible question related...

  4. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    Published
    Feb. 9, 2021, 2:15 p.m.
    ...discuss those ideas and build from those.   Figure 1: Virtual lab meeting? No problem! You can still...final screen can also be saved and exported as a picture. I believe that showing something different from...document can be shared and serve as a springboard for future discussion. Collaboration and creativity are pivotal...

  5. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    Published
    April 19, 2017, 3:25 p.m.
    ...that are then barcoded for identification. This mixture of barcoded fragments is known as a *library*. ...additional benefit of plexWell™’s sequential tagging procedure, the quantities of library molecules for each ...multiplexed pool. The cost of these normalization steps, measured in time and consumables, can be daunting for large...

  6. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    Published
    Dec. 6, 2016, 3:30 p.m.
    ... the library, use next-generation sequencing to ensure that the library has retained gRNA representation...produce high titer lentivirus from the library, ensure gRNA representation in the viral prep, infect target... you're interested in studying. You need to make sure your assay is scalable and robust. What you don'...

  7. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    Published
    April 4, 2023, 1 p.m.
    ...model organism for a few reasons: Affordable to culture and easy to work with Grows extremely fast (doubling...induced by removing methanol from the media. This feature makes the strain tractable for peroxisomal studies... for genome engineering. A unique evolutionary feature of K. phaffii, relative to S. cerevisiae, is that...

  8. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    Published
    March 30, 2023, 1:15 p.m.
    ...receiving only 1 TF expression construct. Cells were cultured following a differentiation protocol before performing...so that a single TF was delivered to each cell, future applications could deliver multiple TFs to each...on the Addgene Blog Learn More about Lentivirus Figure out How to Get the Most from Your Lentiviral Transduction...

  9. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    Published
    Sept. 28, 2016, 2:35 p.m.
    ...they also uniformly stepped back to give a big picture perspective: Dr. Yamanaka talked about his clinical...trial patients with advanced macular degeneration; Laurenz Studer discussed his work with Parkinson's Disease...presenters, it was gratifying to see that Addgene has ensured that plasmid acquisition is not a rate-limiting...

  10. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    Published
    Dec. 20, 2018, 1:15 p.m.
    ...addition to thorough ethical considerations, in making sure that the research has been adequately proven to ...unethical behavior (it’s hard to argue otherwise). To be sure, throughout the history of medical research, there...on necessary actions to review and decide on the future of genome editing technology. We must stop punting...

  11. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

    Type
    Blog Post
    Published
    Nov. 20, 2018, 8:17 p.m.
    ...formats. For example, several journals like eLife, Nature, and Cell use  Research Resource identifiers (RRIDs...resolvable compact identifier. Addgene works hard to ensure that materials available through our repository...Addgene Blog Read a blog post on Our Citations Feature Check out this post on Scientific Reproducibility...

  12. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    Published
    Jan. 12, 2021, 2:15 p.m.
    ...got along well, suggest a coffee chat in the near future. Even just one more meeting (in person or virtually... but I’ll never forget the strawberries. Grand gestures are memorable! Attend events for one organization...defunct (but fun) plug-in called Maps.  Figure 1: See a starfish? This is actually a map of Joanne...

  13. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    Published
    Dec. 18, 2014, 4:06 p.m.
    ...predict protein structures, then you might know a thing or two about computer modeling. Make sure to focus on...? Or perhaps you have presented in some public lectures? These transferable skills could be meaningful...

  14. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    Published
    Oct. 9, 2014, 3 p.m.
    ...see the Introduction to Fluorescence Microscopy lecture at iBiology. Commonly used filter sets & relevant...this filter set and have reported brightness measurements. Here, EGFP outperforms the improved folding...variants, presumably due to the lower growth temperature. This also suggests, however, that there is no...

  15. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    Published
    Feb. 8, 2022, 2:15 p.m.
    ...consolidate several steps into one. Soon you’ll figure out what prompts you need to consistently and accurately... accurately run a protocol.   Figure 1: Evolution of a Western blot protocol, from a full page ...experimental steps are worth doing, and doing well, so make sure you’re taking time to do them carefully and thoroughly...

  16. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    Published
    May 25, 2017, 2:30 p.m.
    ...processes directly affected by light. Long-term light exposure can also lead to cellular phototoxicity, and experimental...substrate like coelenterazine is supplied in the culture medium to allow RLuc8 to produce photons. The original...wavelength to specify which FP emission you’ll capture. Since the Nano-lantern system does not use external...

  17. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    Published
    July 19, 2018, 1:12 p.m.
    ...results you've measured should change established theory. So, what can you do to ensure that your skills...our website and social channels to apply for our future events. In addition to this annual event, graduate...

  18. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    Published
    Sept. 15, 2020, 1:15 p.m.
    ...screening blog post for more information.   Figure 1: Result of a blue-white screen. Image from Stefan...more about colony PCR in this blog post.   Figure 2: The steps of colony PCR.   Sanger sequencing...in this case a plasmid. Sequencing is one of the surest ways to know that your insert is what you expect...

  19. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

    Type
    Blog Post
    Published
    Jan. 30, 2020, 2:15 p.m.
    ...and you think there are four important times to measure that growth rate, you report the results of all...between hypothesis testing and generation, can be sure that results will be reported regardless of outcome...the journal and reviewers are incentivized to make sure that any possible null is really a true null and...

  20. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    Published
    Aug. 6, 2024, 1:15 p.m.
    ...additional flexibility in pseudotyping.   Figure 1: Genome of a prototypical gamma-retrovirus.  ...nucleus through the nuclear pore complex. This feature may be useful for the transduction of terminally...-retroviral plasmids have similar names? The nomenclature of the plasmid backbone is meant to be descriptive...
Showing: 661 - 680 of 886 results