We narrowed to 833 results for: ins
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TypeBlog PostPublishedJan. 8, 2015, 2:34 p.m....series on Management for scientists. This second installment in the Management for Scientists series will ...Walking Around" – don't hide in your office. Stop by instead of emailing. Demonstrate you are open to feedback...
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X-CHIME: Context Dependent Germline Knockout in Immune Cells
TypeBlog PostPublishedJan. 16, 2024, 6:13 p.m....suite of four systems which utilizes custom mouse strains and lentiviral expression vectors (Table 1). The...generation of exhausted CD8+ T cell subpopulations and restrains tumor immunity. Nature Immunology 20, 1335–1347... -
SciComm with the Experts at Science in the News Part 2
TypeBlog PostPublishedApril 27, 2017, 2:30 p.m....Maintaining Institutional Knolwedge and Continuity - SITN's key technique for retaining institutional knowledge... -
Searchable and Sortable gRNAs for Your Next CRISPR Experiment
TypeBlog PostPublishedMay 17, 2016, 2:30 p.m....develop a searchable and sortable datatable that contains validated gRNA sequences. Validated in this context...to deliver your CRISPR components as RNPs (ribonucleoproteins) and therefore usually transcribe your gRNAs... -
CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]
TypeBlog PostPublishedFeb. 18, 2015, 3:09 p.m....CRISPR/Cas9 for targeted genomic deletions We were inspired to produce intrachromosomal deletions based on...copy at a given locus may be useful to study haploinsufficiency. Subsequent modifications at the remaining... -
"What Makes a Good Mentor?" and 6 More FAQs About Science Mentoring
TypeBlog PostPublishedDec. 17, 2013, 2:47 p.m.... own.” “Someone to act as your ‘cheerleader’ – inspire you.” “Also a mentor can give you realistic expectations...mentor does all of these things and more. It is instructive (and entertaining) to review this list of bad... -
2023 and 2024: Reflections and Looking Ahead
TypeBlog PostPublishedJan. 23, 2024, 2:15 p.m....June, we announced a new partnership with the Institute for Protein Innovation (IPI), in which Addgene.... The first IPI collection, 31 rAbs targeting integrins, will be released early this year. In September... -
Cre-ating New Methods for Site-specific Recombination in Drosophila
TypeBlog PostPublishedMay 12, 2015, 1:32 p.m....Kotelevtseva N, King K, Davis JR, Mullins LJ, Chapman KE, Dunbar DR, Mullins JJ. Nucleic Acids Res. 2007;35... -
London Calling - Addgene’s New Europe Office in London
TypeBlog PostPublishedJune 10, 2014, 3:20 p.m....75,000 plasmids to more than 1,500 different institutions in Europe. In the last four years alone, the...fantastic numbers, a lot of European scientists and institutions are not aware of what Addgene can do for them... -
New Viral Vectors - Fall 2024
TypeBlog PostPublishedOct. 3, 2024, 1:15 p.m....If the fall colors are inspiring you to paint your cells in equally vivid colors, you'll want to check...vectors from the Jonathan Ting lab at the Allen Institute, deposited as part of the BRAIN Armamentarium ... -
An Addgene Summer Internship
TypeBlog PostPublishedAug. 8, 2024, 7:22 p.m....toxin, or an oncogene, for safety reasons, and the insert size should be a max of 500 bp (preferably). I ...college and play the drum set as their personal instrument. They are the oldest sibling in a Guatemalan ... -
New CRISPR Web Resources and #12DaysofCRISPR Recap
TypeBlog PostPublishedDec. 12, 2017, 3:13 p.m....demethylation by Tet1. RNA Targeting: This page contains Cas13 constructs for mammalian and bacterial systems...off-target effects! This characteristic probably explains why pC016 - LwCas13a guide expression backbone... -
Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development
TypeBlog PostPublishedNov. 1, 2017, 2:04 p.m....for example, cutting open a heart to look at the inside. We do a lot of interactives and videos, as well... Keown, a staff writer at the non-profit Fred Hutchinson Cancer Research Center. And make sure to subscribe... -
Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes
TypeBlog PostPublishedJune 19, 2018, 1:38 p.m....ARGs) are engineered gene clusters that encode proteins that form bacterial gas vesicles. These ~200nm... plasmids for in vivo imaging with fluorescent proteins Browse luciferase plasmids Find fluorescent protein... -
Running for Rare Disease, Running for FOP, Running for AJ
TypeBlog PostPublishedSept. 15, 2016, 2:30 p.m....Senior Director of Biology, is helping us fight against FOP in two ways. First, Addgene is distributing...plasmids with Addgene and join us in the fight against FOP. Second, Addgene will be hosting the AJ’s Boston... -
Addgene at the ASM Microbe 2016 Conference
TypeBlog PostPublishedJuly 7, 2016, 2:30 p.m....one of the highlights of the conference. It was inspiring to see how microbiology research is improving ...symposia outlined the fantastic work this Broad Institute lab is doing creating well-optimized CRISPR screening... -
10 Ways to Share Your Science!
TypeBlog PostPublishedMay 13, 2014, 8:34 p.m....out to science writers in your area or at your institution; the news or communications office is a good ...internship at a place like Science News. Ask your institutional news office if there might be opportunities ... -
Advice for Choosing a Research Project
TypeBlog PostPublishedApril 17, 2014, 2:45 p.m.... your professional brand. It offers colleagues insight into your interests and will determine how you ...for guidance, intelligent discussion, and/or brainstorming sessions to maximize productivity and direction... -
CRISPR/Cas9 FAQs Answered!
TypeBlog PostPublishedMarch 13, 2014, 4:08 p.m.... What is the maximum amount of DNA that can be inserted into the genome using CRISPR/Cas forHomologous...efficient recombination? A3: The most we've tried to insert so far has been 1kb. We used homology arms that... -
Multifunctional Neural Fibers: Modulation from Gut to Brain
TypeBlog PostPublishedAug. 31, 2023, 1 p.m....sensors, and other common neuroscience tools into the brains of model organisms, including the Anikeeva lab’... Plasmid tools for microbiome studies Channel rhodopsins for optogenetics Viral Vectors 101: Chemogenetics...