We narrowed to 714 results for: met

1. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

   Type

   Blog Post

   Published

   Oct. 29, 2019, 2:52 p.m.

   ... 2018) available at Addgene: MerMAID2 - A metagenomically discovered marine anion-conducting channelrhodopsin...

2. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

   Type

   Blog Post

   Published

   April 12, 2016, 2:30 p.m.

   ...multiple) genetic perturbations in single cells. Methods to scale up multiplexed CRISPR systems for high-throughput...

3. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

   Type

   Blog Post

   Published

   Dec. 16, 2015, 3:30 p.m.

   ...cumbersome, can lower on-target efficiency, and sometimes even increase off-target effects, respectively...

4. New Tool for Lineage Tracing: The ClonTracer Library

   Type

   Blog Post

   Published

   Sept. 22, 2015, 2:30 p.m.

   ... of questions surrounding cancer development, metastasis, and drug resistance, Bhang says “the [drug] ...

5. A Look at Addgene's QC Process

   Type

   Blog Post

   Published

   May 6, 2025, 1:15 p.m.

   ...the plasmid to stop functioning properly or be something completely different than what was originally ...

6. Making CRISPR Plasmids Using Fragmid

   Type

   Blog Post

   Published

   Aug. 29, 2024, 1:15 p.m.

   ...available destination vectors for various delivery methods, along with compatible inserts. All destination...

7. Corralling and Cornering the Goldilocks Antibody

   Type

   Blog Post

   Published

   Aug. 9, 2022, 1:15 p.m.

   ...engineered.   Fig. 1: Antibody production methods    NeuroMab and others have an extensive library...

8. Hot Plasmids and Viral Preps - March 2021

   Type

   Blog Post

   Published

   March 23, 2021, 1:15 p.m.

   ...targeting a specific chromatin feature (e.g. a methylated histone such as H3K27me3), a linker between these...

9. High Complexity Golden Gate Assembly with NEB’s DAD

   Type

   Blog Post

   Published

   Aug. 1, 2023, 1:15 p.m.

   ...highest-fidelity overhang set it can find for those parameters. For even more specificity, you can exclude specific...

10. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type

    Blog Post

    Published

    March 8, 2017, 3:15 p.m.

    ... may enter “donor template vector”. Cloning Method Choose from: Gateway cloning, gene synthesis,...

11. Hot Plasmids - October 2020

    Type

    Blog Post

    Published

    Oct. 13, 2020, 1:15 p.m.

    ...greater number of red cells as measured by flow cytometry. Cells grown in high IL-3 showed mostly blue fluorescence...

12. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type

    Blog Post

    Published

    June 25, 2020, 1:15 p.m.

    ... does. Her parents told her that if she found something she loves to do, she would never work a day in...

13. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type

    Blog Post

    Published

    March 12, 2020, 1:15 p.m.

    ...Krebs, A., Schübeler, D., Hajdú, R.I., Lukáts, Á., Németh, J., Nagy, Z.Z., Wu, K., Wu, R., Xiang, L., Fang...

14. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type

    Blog Post

    Published

    July 24, 2018, 12:51 p.m.

    ...al. Production of lentiviral vectors. Mol Ther Methods Clin Dev. 2016. PubMed PMID: 27110581. PubMed Central...

15. Lab Automation at Addgene

    Type

    Blog Post

    Published

    March 22, 2018, 1:28 p.m.

    ...automate and optimize more protocols in the future - something that will be key for our future growth. One of...

16. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type

    Blog Post

    Published

    Aug. 6, 2019, 12:55 p.m.

    ...chromosomes recombine during meiosis, creating gametes that contain a mix of genetic material from each...

17. Hot Plasmids and Viral Preps - January 2021

    Type

    Blog Post

    Published

    Jan. 5, 2021, 2:15 p.m.

    ...to date By Justin Ng High-throughput screening methods, such as fluorescence-activated cell sorting (FACS...

18. Adapting Toehold Switches to Detect Zika Virus

    Type

    Blog Post

    Published

    Aug. 30, 2016, 2:30 p.m.

    ...the Biodesign Center for Molecular Design and Biomimetics and the School of Molecular Sciences at Arizona...

19. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type

    Blog Post

    Published

    July 14, 2016, 2:30 p.m.

    ... with gRNA sequence. Both groups used multiple methods to examine Cpf1 off-target editing. First, they...

20. Plasmids 101: FLEx Vectors

    Type

    Blog Post

    Published

    April 28, 2016, 2:30 p.m.

    ...allowing for rescued expression of GeneR.   Sometimes also referred to as DiO (Double-floxed inverse...