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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 789 results for: mpl

Showing: 661 - 680 of 789 results

  1. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ... understand the process, we’ll walk through an example experiment where we might use Gateway cloning to...into destination vectors. The cloning process is simple -  no restriction, ligation or gel purification...

  2. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ...avoid rousing the trolls. 3. The AMA process was simple and well organized. You can check out the r/Science...posters (this advice included leaving out the term “implicit bias” which is apparently a troll trigger phrase...

  3. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ... purified from the system, researchers can run samples on SDS-PAGE gels containing Phos-Tag, a small molecule...substitution. Alternatively, you can express the completely wild type protein from the same plasmid.  Upon...

  4. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    Published
    Jan. 26, 2016, 3:30 p.m.
    ...regularly. Dystrophin and genome editing Since complex oligonucleotide treatment comes with many challenges...therapy, it’s important to realize that DMD is a simpler case than other genetic diseases we’d like to treat...

  5. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    Published
    Oct. 2, 2018, 12:56 p.m.
    ...emotions and assumptions. I’m not just talking about simple things like “Which route should I take to get to...choosing a training lab, I strongly suggest you complete a work rotation in the lab if at all possible....

  6. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ...epigenetic modifications across the genome) has been implicated in disease states, and targeting the epigenome...more frequently inherited by daughter cells. For example, KRAB induced silencing was transient and quickly...

  7. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...specific list of the candidate off-target sites. For example, if there are 10 putative off-target locations ...confounding your results should be minimal. For example, if your goal is to generate a knock-out cell line...

  8. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...the cell to use the deaminated DNA strand as a template. To do so, they used a Cas nickase, instead of...repairs the DNA using the U-containing strand as a template, copying the base edit. Figure 2: Cytidine...

  9. New Coomassie Protocol Video

    Type
    Blog Post
    Published
    Oct. 31, 2023, 1:15 p.m.
    ...    If this video is helpful, you can find our complete collection of protocol videos on the Addgene YouTube...

  12. Trends in CRISPR and SynBio Technologies

    Type
    Blog Post
    Published
    Feb. 4, 2015, 3:58 p.m.
    ...sharing In a recent review on CRISPR technology, for example, the authors Patrick Hsu, Eric Lander, and Feng...

  13. Lucky Thirteen: Guest Contributors of 2023

    Type
    Blog Post
    Published
    Dec. 5, 2023, 2:15 p.m.
    ...Created in collaboration with Addgene writers High Complexity Golden Gate Assembly with NEB's DADExperts: Matthew...

  14. 2021 Deck the Lab Winners!

    Type
    Blog Post
    Published
    Jan. 25, 2022, 2:15 p.m.
    ...festive makeover today✂️❄️🦠thanks to the awesome templates by @CVRHutchinson @CVRinfo. Check them out: https...

  15. Congrats to the 2019 Deck The Lab Winners

    Type
    Blog Post
    Published
    Jan. 17, 2020, 3 p.m.
    ...ASCBEMBO19 #MCDB 🎄🎅🎁🐸🐸🐸🐸 pic.twitter.com/2MPLawgNQl — Jennifer Landino (@jenelandino) December 3,...

  16. New Videos: Addgene Lab Tips

    Type
    Blog Post
    Published
    Feb. 15, 2018, 2:41 p.m.
    ..., add the various reagents to a single tube and simply flick the tube several times. This will help ensure...

  17. The Codon Guide to Synthetic Biology

    Type
    Blog Post
    Published
    Sept. 14, 2023, 1:15 p.m.
    ... of the best ways to learn about a new field is simply to know where to look and how to start. There is...
Showing: 661 - 680 of 789 results