...Scientists Making It Happen, is an exercise in restraint. The very first subheading in Chapter 1 asks “..., and he wisely makes no such attempt. He is transparent about his own biases--many of which will be shared...health and environmental science, by the Trump administration are difficulties in dealing with climate change...dire than Zimmer could have imagined. Does this detract from the quality of the book? It doesn’t need to...

...connectivity to open source data repositories. To illustrate this, let’s take a look at the RSpace ELN.  The... commonly used online file storage tools, as illustrated in this video, and to data stored on institutional...is the integration between RSpace and Slack, illustrated in this video. This is a two-way integration....protocols.io, the protocol sharing repository. As illustrated in this video the integration enables RSpace ...

...drafts of the manuscript. This is the time to transform the document into something cohesive-- change ...This is normal and it means you’re on the right track!  Get feedback After you’re done editing, send it...Thompson, and Thomas D. Gilmore. "Histone acetyltransferases and histone deacetylases in B-and T-cell ...6.5-6 (2015): 184. PubMed PMID: 26124919. PubMed Central PMCID: PMC4482241. Additional Resources on the ...

...practice. I always take a poll of scientists in training to see how many in my audiences have given a 50...prepare answers Speaking pros always have a few extra slides in their back pocket. During your practice...else have a good answer for this?” This doesn’t detract from the speaker, but actually makes for a more...session. The best speakers use no notes and can concentrate on delivery, rapport with the audience, and their...

...While this may effectively serve as a transfection or transduction control, a different gRNA would have... Lowering off-target events There are several strategies to lower the predicted frequency of off-target...near each other, this will result in a DNA double strand break (DSB), the event required for frameshift ...

...science, and maybe even win a $500 travel prize in our open data extravaganza!  Anyone can submit data from...presentation, with two winner each receiving a $500 travel award. All participants will receive an Addgene...

...levels to generate anything from individual transcription units (TUs) used to express your favorite gene-of-interest...vectors. The Plant Parts III kit has a focus on transformation and genome engineering. This is a specialized...used successfully for protein localization and transport studies in HEK cells, recombinant protein purification...Rapid Combinatorial Design for Synthetic and Traditional Biology.” ACS Synthetic Biology, vol. 5, no. ...

...Rabies and Neuronal Tracing” blog post! You can also browse tools for AAV tracing, made from rabies plasmids... the international controls prescribed by the Australia Group, for these plasmids for the majority of ...

...Michigan State University 9:36-10:29 : Kingdom's transition back to Malawi and Lilongwe University of Agriculture...-14:53 : Kingdom's current projects producing transgenic crops 14:54-23:44 : Follow up Kingdom's PhD projects... transgenic crops...

...you could directly start your digest, PCR, or transformation?  Great news. A subset of our collection has...going from streaking to a single colony to DNA extraction and quantification so that you can focus on what...280 nm and 260/230 nm ratios) and measure the concentration. Since this is a beta project, it will run for...

...such as bacteria or viruses, which results in neutralization or targeted destruction.  Antibodies are composed...monoclonal antibody against a target antigen. This contrasts with polyclonal antibodies, which are mixtures...antibodies include those directed against human transcription factors such as PAX7, PAX6, AP-2 alpha and ISLET...

... al. (2015). Multiplex genome engineering demonstrated in human cells They used the Golden Gate cloning...cultured human cells. While it has not yet been demonstrated, they say the vectors are also likely to work...The system now provides an efficient targeting strategy for multiplex editing of genomes. It will also...

...learn about intellectual property from our Tech Transfer team members Tony Cova and Chiara DeNatale and...much business there is in this subject.” Outreach travels with Blugene Blugene loves to visit researchers...Scientist Jessica Welch showed some highlights of his travels from 2015, including a great European photo slideshow...

...Addgene's lab. We ship individual plasmids as transformed bacteria in stab culture format. Our lab team...relocated to 75 Sidney Street. Then the plasmids travel to labs around the world. We've shipped plasmids... and collaborations with institutions and tech transfer offices that facilitate MTAs. The great feedback...

...examined the effects of GFP fusions to inntags or traditional FLAG, HA, and Myc tags. Impressively, IT6-GFP...immunoprecipitation applications, but none of the traditional tags were suitable for both applications. To ...morphology was observed with IT5 and IT6 fusions. In contrast, Flag and HA tags had especially detrimental effects...

...gestures are energetic.  This can take practice and training. This is important because it creates a culture...your office. Stop by instead of emailing.    Demonstrate you are open to feedback with your actions     ...listening skills such as the PAC Method. P = demonstrate patience, pause; A = ask at least one question...

...phage matches. Email sstrathdee@ucsd.edu if you can help! — Dr Steffanie Strathdee, 🗡️Superbug Slayer ...species of bacteria and sometimes, only a subset of strains of the same bacterial species are affected. As ...

... as well. Beautiful microscope images which traditionally languished in the pages of thick textbooks are...Queensland, whose outstanding marine photography has attracted much attention over the past few years, including...is easy to see how these incredible images are attracting so much attention. When I spoke with Daniel about...

...scientists. It takes us a decade or more for just the “training” portion of our careers. In that time we may have...organizing mentoring programs for scientists and doing training to help mentors and mentees have fulfilling, productive...wants to be for her new lab. If only every science trainee had such a dedicated person guiding the start of...

...released the MORF collection, a comprehensive transcription factor library, in lentiviral vectors. At 3500...conduct robust investigations into the human transcriptome. The MORF library is only one of 22 libraries...’ll also share our work in evolving Addgene’s strategic vision for the future. Over the years, we have...