...understanding the brain’s function is knowing its architecture, in particular the connections between different...to their advantageous characteristics for brain research and their ability to introduce genetic material...fluorescent proteins (red, green, blue). If a researcher injects a cocktail of these different AAV vectors...Atlas website. A similar approach was used by researchers from the Janelia Campus for their MouseLight ...University of California, Berkeley, the Janelia Research Campus also developed a new AAV variant, rAAV2...

...proteins included in different CGBE tools allow the researcher to direct the repair of the abasic site. But ... originally being developed for A → G edits, researchers tried inhibiting MPG/AAG to improve editing efficiency...transversion editors were developed, though, researchers at HuidaGene Therapeutics guessed that fusing...existing CGBEs. This distinct approach means researchers can now select the best tool for their application...

...in neuronal circuits or synaptic remodeling, researchers combine the Cre and FLP recombinases to control...combination of Cre, FLP, and inducible systems gives a researcher exquisite control over expression of multiple...Katrin Michel! Katrin Michel is a Postdoctoral Researcher at MIT and is fascinated by the question of how...-Lox Check out Retrograde AAVs for Neurscience Research Resources on Addgene.org Find Cre-Lox Plasmids...

...Catharanthus roseus (Figure 1). The ultimate goal of our research is to enhance their production either in the plant...help in screening candidate genes and speeding up research. Certainly, there is still more to transient leaf...your lab, it can become a strong tool for your research. Feel free to add further tips and experiences... in the Lee-Parsons Lab.     References D'Aoust, Marc-André, et al. "Transient expression of antibodies...

...signal at a point on the axis determined by the researcher, based on their controls. You can usually tell...intimated if you’re struggling to understand how the researchers decided to place their gates on the plots! Flow...gating strategy, you can start to ask why the researchers made the decisions they did in their experiments...vitro chondrocytic differentiation: A stem cell research initiative at the King Abdulaziz University, Kingdom...

...and enrich base editing activity. Nucleic Acids Research 48:2841–2852 . https://doi.org/10.1093/nar/gkaa124...cleavage by Cas9 in living cells. Nucleic Acids Research 46:e84–e84 . https://doi.org/10.1093/nar/gky332...enrichment (TREE) in human cells. Nucleic Acids Research 47:e120–e120 . https://doi.org/10.1093/nar/gkz713...the CRISPR guide Find CRISPR plasmids for your research ...

...the College of Life Sciences that same year. Her research is focused on the escape mechanism of genome editing...Molecular Plant Sciences since December 2020. His research focuses on the efficiency of transposon-encoded...professor in Synthetic Biology in 2006. The Yang lab's research is focused on developing microbial genome editing...Sciences in September 2019 as a Ph.D. candidate. Her research focuses mainly on the development and application...

...pooled libraries here at Addgene, including cDNA, barcoding, screening, and gRNA libraries. Most of the pooled... pooled libraries and one of our most popular barcoding libraries as pre-made viral preps. If you can’...polyethyenimine transfection protocol. I also recommend searching PubMed for any publications associated with the...feel more confident incorporating them into your research. And, of course, if you have any questions, don...

...users, we just released a major redesign of how search works on our site. For Addgenies, development includes...with our software. When this happens, we need to research and hopefully find a comparable library if it’...our users focus on the fun stuff, accelerating research and discovery. We work with transparency, gladly...the Addgene blog Learn about what went into our search engine update Find all Addgene news Resources ...

...adaptation for use in mammalian cells in 2012-2013. Researchers are now using this system in ever more creative...Hopefully this post will help get your in vivo research up and running as soon as possible! Selecting ...it’s time to use them in mouse embryos. When researchers first started using CRISPR/Cas9 for genome editing...RNA-guided endonucleases and nickases." Genome research 24.1 (2014): 132-141. PubMed PMID: 24253446. PubMed...