We narrowed to 803 results for: Cre

...Each path to science policy is unique and this creates a rich and diverse workforce. Out of all the career...QUESTION. Science policy is the work you do to creatively connect seemingly disparate individuals and ideas...also happen at the state and local level. The big secret about science policy that I didn’t learn until ...bring researchers to Congress to advocate for increased science funding and other issues. Whether you ... field where you have no specific scientific credentials.  But before you even apply to that first science...

...my partner Mike and I split the work, shared the credit, and we both won accolades that would launch our..., the Senior Scientist who coordinates all kit creation and quality control efforts with depositors; .... We worked with Andrew, the team’s founder, to create a mission statement in the first few meetings and...becoming part of a team is a really good way of creating and strengthening those social bonds. So never...communication options. Addgene, along with an increasing number of academic labs, uses Slack constantly...

... to screen for positive clones in most cases, and restriction enzyme digest can be used to screen plasmids...strand was degraded. Beta: Beta protects the ssDNA created by Exo and promotes its annealing to a complementary...recombination frequency between 0.1% to 1%, and can be increased to as high as 25-50% by designing oligos that ...recombination than dsDNA substrates, so you should screen more clones when using dsDNA. Have you used the...

...be used in concert with in silico prediction to create a more comprehensive list of off-target editing...of DSBs throughout the genome. However, their decreased sensitivity means that the best option moving ...with a focus on the ways science can be used to create a more just and equitable society. Read more at.... Bell, Charles C., et al. "A high-throughput screening strategy for detecting CRISPR-Cas9 induced mutations...Learn to Use Cas9 Activators Perform Genome-Wide Screens with CRISPR Generate Mouse Models with CRISPR/Cas9...

...paths. Check out Joanne's Reddit AMA Here are some concrete things you can do to develop those transferable...Manage a project involving multiple scientists, create and stick to timelines Be a mentor in a formal ...If opportunities don't exist in your department, create them and show your leadership skills too. Teach...are always excellent Technical knowledge and creativity Become a “thought leader,” develop a technical...

... drug discovery by big pharma (i.e. HTRF and LanthaScreen) (Degorce et al., 2009), measuring intracellular...are critical in setting up a FRET-based assay or screen (the Addgene blog has an excellent primer on FRET... biomolecules each containing multiple binding crevices that can nonspecifically soak up your fluorophore...donors (Fig. 1). Scores of FP variants have been created, thereby enabling a broad spectrum of donor fluorescence...strategies. FRET experimental design lends itself to creativity and innovation, valuable commodities in any NIH...

...COVID-19 research? We decided to give it a try, by creating a service for COVID-19 researchers. So far, we...to researchers worldwide. “COVID-19 has spread incredibly fast and without any notion of national boundaries...-19. “Open scientific sharing is important for creating research synergies and accelerating the translational... but we believe that by coming together, we can create a significant impact on both clinical care and ...

... The MORF library is only one of 22 libraries, created from 14 deposits, that became available through...would be remiss not to mention that 2024 is an incredibly special year for Addgene: it marks our 20th anniversary...twenty years while seizing new opportunities to create even greater value for the scientific community...every Addgenie, thank you for twenty years of incredible success, and for believing, as we do, that sharing...

...including Andrea Radtke, PhD, and Ziv Yaniv, PhD, have created the IBEX Knowledge-Base, a community repository...from the Zenodo dataset.     Figure 2: Screenshot showing reagent entries with associated metadata...others in the IBEX community.   Figure 3: Screenshot showing recent topics in the IBEX discussion ...when designing the IBEX Knowledge-Base, seeking to create an iterative process that is constantly becoming...

...kit size, it may be tempting to try increasing your input to increase your output. But, exceeding a kit’...their relative qualities and quantities. Image created in BioRender.com. Transformation Grade DNA...that you are left with pure plasmid DNA. Image created in BioRender.com. For the clean-up stage, plasmid...kit.   Do you want eco-friendly options? It’s no secret that labs produce a lot of waste. If you are looking...