...materials via Addgene. Perhaps if each of many grantors supporting research could provide Addgene with a small...Zuckerberg Initiative for supporting Addgene by providing a generous grant. This support will enable us ...sent the Addgenies home.  At the same time, as Addgene started dealing with this new reality of working...resource for scientists studying the coronavirus. Addgene’s Technology Transfer Team also moved fast to ensure...science sharing as we can get. The effects of Addgene’s brief shut-down and the subsequent fluctuations...don’t have space in this blog to call out every Addgene Team because they’re all doing amazing work during...rose to the challenge was our Finance Team. With Addgene closed for almost two weeks and with labs worldwide...

...versions of each, and Tim Lu even compared his "memory" gene codes to a Turing machine (also discussed in the...Doudna, Feng Zhang, and Keith Joung. All Addgene depositors! Addgene was mentioned specifically in Dr. Zhang's...harnessed. Sharing CRISPR, synbio, & more via Addgene Addgene's addition to the poster session was a first...attendant called them, "long, skinny things") and generally exuding a very-tired-but-very-excited attitude...happens): Science is hard. Chris Voigt, another Addgene depositor, discussed some of the many challenges...That so many scientists are tackling this type of genetic engineering is a testament to how amazing the downstream...from the custom software used to design complex genetic circuitry to the downstream output. Tuesday morning's...

...contacting Addgene can be found on our Contact page. You’ve called. You’ve emailed. And Addgene has been...Plasmid sharing in Europe: Addgene’s request statistics Since 2004, Addgene has shipped more than 75,000...into the Addgene repository. More than 4,500 plasmids have already been deposited at Addgene by over 250... your service: Addgene’s London office Just a few weeks ago I became the newest Addgene Outreach Scientist...UPDATE - As of January 2020, Addgene's Europe office no longer handles customer support inquiries. The...Cambridge, MA to be exact. Today, that changes. Today, Addgene launches a brand new Europe office located in London...poised and ready to answer your questions about Addgene, plasmids, and depositing (all while our US counterparts...

...frequently requested. Ubiquitin - One particular gene we saw increased interest in from 2008 to 2009 was...expression and pLVTHM, a lentiviral plasmid for mammalian gene silencing. TALENs - Genome engineering methods improved...Addgene was founded 10 years ago today. In that time, Addgene has shipped over 350,000 individual plasmids...biosensors, optogenetics, and other imaging tools. The GCaMP5 and GCaMP6 series of genetically encoded calcium...Top 10 plasmid technologies distributed through Addgene over our first 10 years. 10 most distributed plasmid...it's not actually a technology, but pSV-PGC1 was Addgene's first distributed plasmid and was created in the...repository. 100,000 Plasmids Shipped! - By 2010, Addgene had hit our stride. We shipped our 100,000th plasmid...

...When I got the job of Outreach Scientist at Addgene, my first thought was “I get to talk to scientists... spend a lot of time telling scientists about Addgene’s repository, and explaining how to deposit and ... 1,600+ labs around the world!) that have kept Addgene at the forefront of life sciences research for ...from scientists who have deposited plasmids at Addgene.  Learn how discoveries were made and get practical...scientific sharing and the open-access movement. At Addgene, we believe that science gets better when everyone... to tell? Let us know by emailing us at blog@addgene.org or tell us on Twitter and Facebook. We’ll track...

...international researcher who uses plasmids containing genes from rabies and vesicular stomatitis virus, you ... export control exceptions for rabies and VSV-G genes, plasmids ship out approximately three weeks faster...ordering plasmids, please feel free to email help@addgene.org. We’ll be happy to help! A quick note: While ...complex and may change suddenly. Please email help@addgene.org for the most-up-to-date information on shipping...

...Projects in Wei Leong Chew's lab. Genome engineering, gene delivery methods, AAVs, and more. Chapter 7/Outro... in the areas of genome editing, immunology, and gene therapy.   Special thanks to our sound engineer/...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.      Additional...Additional Resources from the Addgene Blog Visit our CRISPR featured topic page Check out our Hot Plasmids...synthetic biology researcher Tom Ellis Resources at Addgene.org Find CRISPR Plasmids Find Synthetic Biology ... addgene podcast...

...Hybrid Serotypes: Improved Vectors for Gene Delivery. Curr Gene Ther 5(3), 299–310. PubMed PMID: 15975007...vectors could also be powerful tools for the field of gene therapy if they can reach the affected part of the...anterograde and retrograde axonal transport. Hum Gene Ther 25, 705–720. PubMed PMID: 24694006. PubMed ...2017). Engineered AAVs for efficient noninvasive gene delivery to the central and peripheral nervous systems...., and Nakahara, K. (2011). Local and retrograde gene transfer into primate neuronal pathways via adeno-associated...-associated viral vectors is serotype-dependent. Gene Ther 20(3), 348–352. PubMed PMID: 22418061. PubMed...vector serotypes 1, 2, 5, 7 and 8 in mouse brain. Hum Gene Ther 18, 195–206. PubMed PMID: 17343566. Tervo, ...

...sequence to confirm backbone elements.   Confirming the gene/insert by aligning to NCBI entry or using BLAST....available reference sequence or plasmids containing genes with many modifications. We look for point mutations...amino acid. We also confirm that the species of the gene matches the data associated with the plasmid.   ...Viral Preparations from Viral DNA Extracts. Human Gene Therapy 31:664–678 . https://doi.org/10.1089/hum...for all incoming plasmids was a big change for Addgene - we had used Sanger sequencing for quality control...control for over ten years. Now, thanks to a generous donation by the Hirsh family, we are able to take...provide requesting scientists high quality data! Addgene's new in-house NGS process To start, the process...

...control expression of their genes of interest. While recombinases are powerful gene expression tools in and...CRISPR knockout library that targets human epigenetic genes was deposited by Kivanc Birsoy. Find the ...blue-light inducible Cre for precise control of bacterial gene expression By Meghan Rego Recombinases are enzymes...precisely controlled through the use of optogenetics. Optogenetic tools use light to induce conformational...Synthetic Biology publication, Mary Dunlop’s lab generated a photosensitive Cre, Opto-Cre-Vvd, by splitting...from Biggiephage can eliminate competing mobile genetic elements. Image by Basem Al-Shayeb in the Doudna...unique spatiotemporal phenotypes from large heterogeneous cultures.  HeLa cells expressed a fusion...