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We narrowed to 928 results for: Gene

Showing: 341 - 350 of 928 results

  1. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    Published
    July 30, 2024, 1:15 p.m.
    ...in the Addgene repository each year from 2016–2023. B) AAV preps distributed per year by Addgene from 2016...vector preps contain biosensors, optogenetic tools, and chemogenetic tools: protein tools that are activated...available at Addgene from 2016–2023 by type of tool. B) AAV preps distributed by Addgene per year from...biosensor, by Addgene’s categorization) was first developed in 2001, with successive generations developed...As part of Addgene’s 20th anniversary celebration, we’ve been finding and sharing stories unique to our... our repository and resources. As editor of Addgene’s eBooks — including the recently released Viral Vectors...viruses engineered to be safely used to deliver a genetic payload to cells, and the complexity of effectively...

  2. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    Published
    Nov. 3, 2020, 2:15 p.m.
    ...delivery enables minimally-invasive gene delivery and optogenetic excitation. Excitation of neurons deeper...the differences between chemogenetics and optogenetics Resources on Addgene.org Browse all viral vectors...available at Addgene Visit our viral vectors guide Find our chemogenetics and optogenetics science guides...resources on the Addgene blog Read more about the PHP.eB AAV capsid Read more about optogenetics Learn about...Optogenetics is a neuroscience method that lets you fire neurons with the flick of a light switch. Neurons...allowing an influx of ions into the neuron which generates an action potential (Figure 1). This light-induced... to use invasive measures to perform in vivo optogenetic experiments. Intracranial injections of AAV are...

  3. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    Published
    Sept. 17, 2020, 1:15 p.m.
    ...materials via Addgene. Perhaps if each of many grantors supporting research could provide Addgene with a small...Zuckerberg Initiative for supporting Addgene by providing a generous grant. This support will enable us ...sent the Addgenies home.  At the same time, as Addgene started dealing with this new reality of working...resource for scientists studying the coronavirus. Addgene’s Technology Transfer Team also moved fast to ensure...science sharing as we can get. The effects of Addgene’s brief shut-down and the subsequent fluctuations...don’t have space in this blog to call out every Addgene Team because they’re all doing amazing work during...rose to the challenge was our Finance Team. With Addgene closed for almost two weeks and with labs worldwide...

  4. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    Published
    Dec. 12, 2019, 2:22 p.m.
    ...scientific domain in general and of our data in particular and our deep understanding of Addgene’s customer’s needs...’t. Try out the new Addgene search engine! Additional resources from the Addgene blog Learn about other...other features on Addgene's website Find a step-by-step guide to using Addgene's new search Read to learn... that we needed to improve more than that: the general “findability” of the information on the site. Different...posts or reagent items.  The technology behind Addgene’s new search  Like millions of other websites, we...approach much more than if we were dealing with general purpose information. Below are a few of the strategies...

  5. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ... Figure 1: In conventional gene targeting (A), you must first generate your desired mutation in mouse...mutations, the 8-10 month process of conventional gene targeting is lowered to about 2-3 months. But certain...example of inserting a 2A-FlpO cassette into the Fgf8 gene. Miura et al. (2018) suggest using a 50-80 bp sequence... mouse model generation easi-er! Generating mice before CRISPR The process of generating knock-in mice...process of mouse model generation simpler, as described in an earlier post on generating CRISPR mouse models...after editing. How to use Addgene plasmids for Easi-CRISPR You can use Addgene plasmids to create a repair...template. Figure 2: Overview of ivTRT to generate an ssDNA repair template. Although effective...

  6. Activate Addgene's "Find Plasmids" Badge on Pubmed Abstracts

    Type
    Blog Post
    Published
    May 12, 2017, 12:58 p.m.
    ...plasmids deposited at Addgene from the abstract page. Check out that beautiful Addgene “Find Plasmids” badge...LinkOut” and search for “Addgene”. Then check the “Link Icon” box next to the “Addgene Nonprofit plasmid repository...Manuscript Resources on Addgene.org Learn How to Order Plasmids from Addgene Check Out Our FAQ Pages ...badge to be taken directly to the plasmids on Addgene’s site (Side note: I always recommend you read the...plasmids badge appear So how do you make this magical Addgene badge appear? First you’ll need to create an account... hope this helps! Additional Resources on the Addgene Blog Get Tips on Keeping Up with the Literature... addgene...

  7. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    Published
    March 23, 2017, 2:30 p.m.
    ...expand Addgene’s services. There was an overwhelmingly positive response to the option of Addgene providing...allows us to maintain Addgene’s high quality standards?” Under the leadership of Addgene’s co-founder, Melina...In the middle of 2016 Addgene started distributing a small but growing catalog of ready-made AAV and ...Lentiviral preps. This new Viral Service represents Addgene’s largest new initiative since we started distributing... capabilities to produce and distribute virus. Addgene is a 501(c)3 nonprofit and our plasmid service ...build a completely self-supporting service so that Addgene is not dependent on grants. Aiming to operate under...Sponsors and Supporters Page Thank you to all of our generous, sharing depositors. Over 3,000 laboratories are...

  8. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    Published
    Feb. 22, 2017, 3:30 p.m.
    ... orientation as the gene or use custom gene synthesis or site directed mutagenesis to remove the BbvCI...this. 2) When your gene of interest has a BbvCI site(s). If so, the nicking mutagenesis protocol will still...work if the orientation of the BbvCI site(s) of the gene are in the same orientation as those in the plasmid...Additional Resources on the Addgene Blog Use Site Directed Mutagenesis to Generate Specific Mutations in Your... pot saturation mutagenesis as a quick and easy technique that can be used to generate complex libraries...of this. Plasmid library generation prior to one pot saturation mutagenesis Several methods can be used...effects. One-pot saturation mutagenesis The one-pot saturation mutagenesis technique described in Wrenbeck...

  9. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    Published
    Nov. 17, 2016, 3:30 p.m.
    ...Versatile Tool for Gene Expression in Mammals Additional Resources on Addgene.org Overview of Viral ...pooled libraries. Whether you’d like to knockout a gene, replace it, modulate its expression, or perform...while rtTA can be used to activate expression of a gene under the control of the tet operator. AAV AAVs ...associated virus (AAV, primarily chemogenetics tools for now but with optogenetic tools coming soon). The viral...excited that Addgene is now offering virion preparations to the scientific community. Addgene's AAV preparations...posts on the blog and pages on the Addgene website. blog.addgene.org The blog provides tips for working...email us and we’ll get you writing!     www.addgene.org On the Addgene website you’ll find our updated viral...

  10. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    Published
    Aug. 9, 2016, 2:30 p.m.
    ...which NANOG-related genes are critical to stem cell survival. Knowing which genes influence the survival...compaction, an enhancer can increase the expression of a gene that is millions of base pairs away. (For details...GPS for the genome” to compute coordinates for genetic elements like protein, RNA, and DNA (Table 1). ...family as HIV. By design, our lentivirii are genetically programmed to CRISPR out the enhancers we identified...differentiate over time, we periodically use next-generation sequencing to measure the relative proportion...pillar and the ESC dies. Order Lentivirus from Addgene If you’re interested in designing your own CRISPR...Hypersensitive Sites and Intergenic CRISPRs Yours truly generated the DHS data set by starting with all of the DHS...
Showing: 341 - 350 of 928 results