...process was working as expected, we moved forward with creating the next process and linking the output...needs of the user. Thus, a key criteria we were looking for was the ability to re-use or borrow existing... end of the script or by opening the files. By working on one process at a time, and testing a small set...

...appealing, but also scary. I do a lot of public speaking where I enjoy communicating face to face with ...early and brush up on best practices for LinkedIn networking. I was an early adopter of the LinkedIn blog ...ve said this before, but I also highly recommend taking every opportunity you get to speak publically about...Share Your Science 6 Tools & Tips: Online Social Networking for Scientists Sharing Your Lab Protocols: Using...

...forever!  This can be an advantage for researchers looking to generate long-lasting changes in cells. However...inserting viral DNA into the host genome. Without a working integrase, IDLVs can still deliver their genetic...Considerations for IDLV use If you’re reading this and thinking “Gee, I’d love to give these a try myself,” look...Linch, D. C., Bayford, J., Brown, L., Quaye, M., Kinnon, C., Ancliff, P., Webb, D. K., Schmidt, M., von...

...situation differently, some adopting simple note-taking tools, others using excel sheets or organizing ... on the market today, ranging from general note taking tools, to more scientific field-based solutions...of use of the software. We identified that when talking about ELN costs and limited budgets, “a large percentage...ELN will support that. In addition, if you are working for a large institution or company, site licenses...

...experiment. What is immunofluorescence? Generally speaking, IF is an application in which antibodies that... different approaches to be familiar with when talking about IF, direct and indirect. For direct IF, researchers...different colors. Multiplex immunofluorescence Speaking of different colors, having a palette of secondary...permeabilization, antigen retrieval, and sectioning. Blocking Block is used to reduce non-specific binding...

...Iannaccone et al., 2009). Rats are larger than mice making them more useful for certain studies that involve...genetics and developmental patterns are conserved, making them great models for human disease research. One...eggs are large, opaque, and produced in abundance, making them useful for in vivo studies. These attributes...flowers. Image from BlueRidgeKitties. If you are working with Arabidopsis check out the GreenGate Toolkit...

...fit a big gene into a tiny vector like AAV? By breaking the transgene into smaller pieces. Reassembly ...following transduction.  So what’s going on here? The working model is that the oversized AAV genomes are fragmented...Surveillance Mechanism That Eliminates Transcripts Lacking Termination Codons. Science 295:2258–2261 . https... Hirsch ML, Li C, Bellon I, Yin C, Chavala S, Pryadkina M, Richard I, Samulski RJ (2013) Oversized AAV...

...Brainbow labeling has also been applied to cell marking and lineage tracing in other systems, such as zebrafish...directly trafficked to cell membranes. Membrane trafficking of farnesylated derivatives enables labeling ...fluorescence due to the addition of translation-blocking STOP cassette immediately following the promoter...dev.094631. PubMed. Multiplex cell and lineage tracking with combinatorial labels. Loulier K, Barry R,...

...this post, we didn’t come across a broadly used working definition of systemic capsids. After some consideration...Beadle, K., Jang, M.J., Kim, H.M., Rajendran, P.S., Tompkins, J.D., Shivkumar, K., Deverman, B.E., Gradinaru...Chen, X., Wolfe, D. A., Bindu, D. S., Zhang, M., Taskin, N., Goertsen, D., Shay, T. F., Sullivan, E. E.... I. G., Pacouret, S., Chen, A. T., Chan, Y. A., Ryvkin, D., Gu, C., & Deverman, B. E. (2022). A high-efficiency...C., Lagerborg, K. A., Tellez, L., Krunnfusz, A., King, E. M., Ye, S., Solomon, I. H., Tabebordbar, M.,... Tabebordbar, M., Lagerborg, K. A., Stanton, A., King, E. M., Ye, S., Tellez, L., Krunnfusz, A., Tavakoli...

... channel with fast kinetics may be required, whereas a channel with slower kinetics can be used for lower...each light source used.   Channel and activation kinetics Some channels turn on and off very rapidly in ...thorough characterization data, like spectral and kinetic activation information, in the supplemental data...