We narrowed to 904 results for: ANT

...left not really knowing what I wanted to do, but knowing that I wanted to leave the bench.” Her path to...called “Teaching Your Research.” This course has participants take their research from the bench to the classroom...

... organisms: mammalian cells, yeast, drosophila, plants, worms, zebrafish, frogs, some bacteria; but not...has maximum activity from 50-70 °C. The key advantage of GeoCas9 is that it functions at temperatures...yet? Let us know in the comments below. And if you want to give GeoCas9 a try, as always, the plasmids are...

...popularity of single-use items, plastic is a substantial portion of lab waste. Despite the fact that researchers...challenge you to take to action! These days, it’s important for individuals, companies, and universities to...to work on this issue. Be a champion for this important cause! To get more lab sustainability tips, check...

... zones, it’s important that everyone can access data and drafts of manuscripts and grant applications ...directions of the project. The state of research is constantly shifting, and long-distance collaborations are...

...worldwide and the presentation made it clear how important it is for us to improve our resources for this... meet hundreds of the over 11,000 conference registrants, including scientists from six continents who...presentation at the CRISPR screens symposia outlined the fantastic work this Broad Institute lab is doing creating...

...invading phages. Rising to the challenge - Evolution of anti-CRISPR genes in phages The CRISPR/Cas adaptive immune...CRISPR/Cas. Earlier this year, expression of 1 of 5 anti-CRISPR genes were found to inactivate the Type I-F... the presence of a variety of undiscovered viral anti-CRISPR genes. These data highlight an interesting...

...evaluating CRISPR technologies, including the ever important on/off-target efficiency. Likewise, the NIST Genome... template that contains your desired edit, and I want to make sure that I (and Addgene) don’t forget this...Check out this post to see if an engineered Cas9 variant might work for your experiment. PITChing MMEJ as...

...I didn’t truly address the question of what I wanted to do when I grew up until I was all but finished... I was struck with the realisation that I didn’t want a career in neuroscience—whatever that entails. ... this subject allowed me to engage in findings relevant to my background (neuroimaging, biomechanics, ...

...is the biological outcome you want to achieve? For example, do you want the nucleic acid cargo to be integrated... If you know the basics but are here because you want to know the intricacies of transductions, we've ...

...to identify specific cells — as well as their descendants. This makes them useful for lineage tracing, ...BioRender.com. The CellTag libraries from the Samantha Morris lab use a combinatorial barcoding approach...protein, teaching us more about viral evolution and antibody binding. Figure 3: Schematic of phage display...

...teenager in high school, I knew that this is what I wanted to do,” Stegmeier says He began this journey in...wealth of resources on Addgene’s website and blog important. “The experience from the scientific community... either through direct industry collaborations, grants, or setting up commercial and nonprofit companies...

... of the main tenets of our quality control (QC) mantra is to do our utmost to ensure that scientists are... containing just the expression cassette that we want, it also includes a small amount of spurious DNA...packaging plasmids. We decided to use this to our advantage by designing a Python script that looks for pre-defined...

...cultures in the shakerTurn the LEDs onAnd when you wantYou can just turn them offNo need for any washNo need... aureochrome1 (VfAu1), led to functional BLADE variants, too. The team tested different linkers between...light without the need of any cloning.     What advantages does BLADE have over AraC? When compared to the...

...enjoy, remember, and listen to narratives — take advantage of that in your work The part of my job I most...add author details a different person in the lab wanted to be the first author. When I raised this, he ... likely to be memorable. Work out what story you want to tell; tell it; stop. Many thanks to our guest...

...fuel sources.” In her work, Dr. Polka made R-body mutants that respond to different pH’s and some that are...School describes how AAVs function and indicates important things to consider if you’re getting started with...through Addgene.  Thanks again to all of our fantastic depositors and guest writers. Your contributions...

...xOtH5bs4HiI Advantages of MG-2I-dL5** over other photosensitizers One of the main advantages of MG-2I-dL5... Have you ever wanted to selectively kill a subset of cells in your model system? Turns out that with...

...be it an organic dye or FP) is one of the most important steps in the design of an imaging experiment.  ...Goedhart on the Addgene blog – outlining various important considerations when choosing a FP, but much of...valid email address).  One may also simply attach important references that serve to further characterize ...

... many aspects of ApoB-LP biology, including quantification of abundance, size, and localization in vivo...dependent and the system may not be adaptable to distantly related species from E. coli. This tool should...be performed in intact animals and offers many advantages over traditional measurements such as patch clamp...

...SARS-CoV-2 were developed for broad use and are now important for accelerating research into this virus. One...scientific community. Addgene was awarded a Fast Grant to ensure that the scientific community will continue...Deposits of new reagents, policies from journals and granting agencies requiring materials to be deposited into...

...CRISPR tools, without compromising their strategic advantages in gene editing, while keeping the system to ... array with a given tRNA (derived from human or plant source), by tuning the length of 5’ leader sequences...outperformed other tRNA candidates (either from human or plant sources) in both the strength as a promoter and ...