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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 890 results for: ANT

Showing: 681 - 700 of 890 results

  1. The Strength of Story Telling

    Type
    Blog Post
    Published
    Dec. 19, 2017, 2 p.m.
    ...enjoy, remember, and listen to narratives — take advantage of that in your work The part of my job I most...add author details a different person in the lab wanted to be the first author. When I raised this, he ... likely to be memorable. Work out what story you want to tell; tell it; stop. Many thanks to our guest...

  2. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    Published
    Nov. 18, 2016, 3 p.m.
    ...fuel sources.” In her work, Dr. Polka made R-body mutants that respond to different pH’s and some that are...School describes how AAVs function and indicates important things to consider if you’re getting started with...through Addgene.  Thanks again to all of our fantastic depositors and guest writers. Your contributions...

  3. Viral Vectors 101: Transductions

    Type
    Blog Post
    Published
    April 30, 2024, 1:15 p.m.
    ...is the biological outcome you want to achieve? For example, do you want the nucleic acid cargo to be integrated... If you know the basics but are here because you want to know the intricacies of transductions, we've ...

  4. DAP arrays for multiplex gene editing

    Type
    Blog Post
    Published
    July 7, 2022, 1:15 p.m.
    ...CRISPR tools, without compromising their strategic advantages in gene editing, while keeping the system to ... array with a given tRNA (derived from human or plant source), by tuning the length of 5’ leader sequences...outperformed other tRNA candidates (either from human or plant sources) in both the strength as a promoter and ...

  5. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type
    Blog Post
    Published
    April 28, 2021, 1:15 p.m.
    ...screen and transform your work into a paper or a grant. Here’s a list of tools and resources that might...differences between these two citation managers. If you want more tips on finding and organizing papers, check...post: Early Career Researcher Toolbox: Finding Relevant Papers. These are only a few of the many writing...

  6. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    Published
    Aug. 4, 2020, 1:15 p.m.
    ...SARS-CoV-2 were developed for broad use and are now important for accelerating research into this virus. One...scientific community. Addgene was awarded a Fast Grant to ensure that the scientific community will continue...Deposits of new reagents, policies from journals and granting agencies requiring materials to be deposited into...

  8. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    Published
    May 16, 2018, 1 p.m.
    ...be it an organic dye or FP) is one of the most important steps in the design of an imaging experiment.  ...Goedhart on the Addgene blog – outlining various important considerations when choosing a FP, but much of...valid email address).  One may also simply attach important references that serve to further characterize ...

  9. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    Published
    Dec. 6, 2016, 3:30 p.m.
    ... worthwhile. Particularly if in the end all they want to do is to quickly generate a single cell line ...." If you ever run out of ready-to-use virus and want to make new lentiviral preps on your own, fear not...excited to provide this new service for researchers wanting an easier way to use CRISPR and would love to hear...

  10. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type
    Blog Post
    Published
    Sept. 19, 2017, 1:20 p.m.
    ...xOtH5bs4HiI Advantages of MG-2I-dL5** over other photosensitizers One of the main advantages of MG-2I-dL5... Have you ever wanted to selectively kill a subset of cells in your model system? Turns out that with...

  11. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    Published
    Sept. 28, 2016, 2:35 p.m.
    ...be of secondary importance (or tertiary, or non-existant). On the very first night of the iPSC symposium... the stem cell community for addressing these important questions that the scientific method cannot address... it took the joint effort of five post-docs to implant human kidney progenitors into a pig. In stark contrast...

  12. Management for Scientists: Delegating is Key

    Type
    Blog Post
    Published
    Feb. 20, 2015, 8:30 p.m.
    ...get more done. There are also a host of other advantages to practicing effective delegation skills: ...responsibility if things go wrong. Here are some important criteria for delegating tasks appropriately: ... and accountability? What types of issues do you want to know about right away? Provide adequate support...

  13. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    Published
    Dec. 18, 2014, 4:06 p.m.
    ...understanding of the topic will give you some advantage over other candidates. Write about your transferable...research. For example, did you help put together a grant application? Are you familiar with the publishing...whether to stay in academia or not, it is even more important to start early. Think about your plan for the ...

  15. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type
    Blog Post
    Published
    May 3, 2018, 1:48 p.m.
    ...immunogenicity. But these studies also identified other advantages, including Cas13d-specific regulatory proteins... makes WYL proteins an intriguing counterpart to anti-CRISPR proteins that negatively modulate the activity...efficiency; where shRNA treatment produced 500-900 significant off-targets, CasRx displayed zero. Unlike Cas9...

  17. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    Published
    Dec. 11, 2018, 12:46 p.m.
    ...handle, but I wasn’t using them to my professional advantage. It wasn’t until I wrote a blog post about a research...start using social media to their professional advantage, I’ve selected five resources that have tips for...can fit into a researcher’s work day. It’s also important to remember that it’s not the amount of time you...

  18. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ...polymerase. E4/E1A activate transcription of other relevant viral genes (including the ones listed previously... it to evade detection by the host. Of note, recombinant adenovirus is missing the endogenous E1 gene,...services for some of the plasmids in our collection. Want to make a vector construct on your own? No problem...

  19. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    Published
    March 21, 2023, 1:15 p.m.
    ...allows you to infect cells and deliver cargo, it’s important to understand their differences to choose the ...used to achieve transient, high expression of the relevant cargo without the fear of random genomic integration...non-integrating and infect non-dividing cells. AAVs primary advantage over adenovirus is their low immunogenicity, which...

  20. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    Published
    June 29, 2017, 1:06 p.m.
    ... a variety of common plasmid features such as antibiotic resistance genes, selectable markers, promoters... files Once you’ve identified a plasmid that you want to learn more about, updates to our sequence analyzer...used to indicate them on the map, direction (if relevant), and types. Types listed are standard GenBank...
Showing: 681 - 700 of 890 results