We narrowed to 897 results for: ANT

...evaluating CRISPR technologies, including the ever important on/off-target efficiency. Likewise, the NIST Genome... template that contains your desired edit, and I want to make sure that I (and Addgene) don’t forget this...Check out this post to see if an engineered Cas9 variant might work for your experiment. PITChing MMEJ as...

...enjoy, remember, and listen to narratives — take advantage of that in your work The part of my job I most...add author details a different person in the lab wanted to be the first author. When I raised this, he ... likely to be memorable. Work out what story you want to tell; tell it; stop. Many thanks to our guest...

...is the biological outcome you want to achieve? For example, do you want the nucleic acid cargo to be integrated... If you know the basics but are here because you want to know the intricacies of transductions, we've ...

...teenager in high school, I knew that this is what I wanted to do,” Stegmeier says He began this journey in...wealth of resources on Addgene’s website and blog important. “The experience from the scientific community... either through direct industry collaborations, grants, or setting up commercial and nonprofit companies...

... of the main tenets of our quality control (QC) mantra is to do our utmost to ensure that scientists are... containing just the expression cassette that we want, it also includes a small amount of spurious DNA...packaging plasmids. We decided to use this to our advantage by designing a Python script that looks for pre-defined...

...I didn’t truly address the question of what I wanted to do when I grew up until I was all but finished... I was struck with the realisation that I didn’t want a career in neuroscience—whatever that entails. ... this subject allowed me to engage in findings relevant to my background (neuroimaging, biomechanics, ...

...cultures in the shakerTurn the LEDs onAnd when you wantYou can just turn them offNo need for any washNo need... aureochrome1 (VfAu1), led to functional BLADE variants, too. The team tested different linkers between...light without the need of any cloning.     What advantages does BLADE have over AraC? When compared to the...

...fuel sources.” In her work, Dr. Polka made R-body mutants that respond to different pH’s and some that are...School describes how AAVs function and indicates important things to consider if you’re getting started with...through Addgene.  Thanks again to all of our fantastic depositors and guest writers. Your contributions...

...be it an organic dye or FP) is one of the most important steps in the design of an imaging experiment.  ...Goedhart on the Addgene blog – outlining various important considerations when choosing a FP, but much of...valid email address).  One may also simply attach important references that serve to further characterize ...

...xOtH5bs4HiI Advantages of MG-2I-dL5** over other photosensitizers One of the main advantages of MG-2I-dL5... Have you ever wanted to selectively kill a subset of cells in your model system? Turns out that with...

...SARS-CoV-2 were developed for broad use and are now important for accelerating research into this virus. One...scientific community. Addgene was awarded a Fast Grant to ensure that the scientific community will continue...Deposits of new reagents, policies from journals and granting agencies requiring materials to be deposited into...

...CRISPR tools, without compromising their strategic advantages in gene editing, while keeping the system to ... array with a given tRNA (derived from human or plant source), by tuning the length of 5’ leader sequences...outperformed other tRNA candidates (either from human or plant sources) in both the strength as a promoter and ...

...screen and transform your work into a paper or a grant. Here’s a list of tools and resources that might...differences between these two citation managers. If you want more tips on finding and organizing papers, check...post: Early Career Researcher Toolbox: Finding Relevant Papers. These are only a few of the many writing...

... many aspects of ApoB-LP biology, including quantification of abundance, size, and localization in vivo...dependent and the system may not be adaptable to distantly related species from E. coli. This tool should...be performed in intact animals and offers many advantages over traditional measurements such as patch clamp...

... worthwhile. Particularly if in the end all they want to do is to quickly generate a single cell line ...." If you ever run out of ready-to-use virus and want to make new lentiviral preps on your own, fear not...excited to provide this new service for researchers wanting an easier way to use CRISPR and would love to hear...

...be of secondary importance (or tertiary, or non-existant). On the very first night of the iPSC symposium... the stem cell community for addressing these important questions that the scientific method cannot address... it took the joint effort of five post-docs to implant human kidney progenitors into a pig. In stark contrast...

...get more done. There are also a host of other advantages to practicing effective delegation skills: ...responsibility if things go wrong. Here are some important criteria for delegating tasks appropriately: ... and accountability? What types of issues do you want to know about right away? Provide adequate support...

...understanding of the topic will give you some advantage over other candidates. Write about your transferable...research. For example, did you help put together a grant application? Are you familiar with the publishing...whether to stay in academia or not, it is even more important to start early. Think about your plan for the ...

...immunogenicity. But these studies also identified other advantages, including Cas13d-specific regulatory proteins... makes WYL proteins an intriguing counterpart to anti-CRISPR proteins that negatively modulate the activity...efficiency; where shRNA treatment produced 500-900 significant off-targets, CasRx displayed zero. Unlike Cas9...

...cloning: A SLIC makeover In 2007, LIC received an important update, courtesy of Addgene depositor Stephen ...structures at the ends of fragments. The major advantage of SLIC over Gibson assembly is cost, as T4 polymerase...homologous recombination in vitro to generate recombinant DNA via SLIC. Li MZ, Elledge SJ. Nat Methods....