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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 889 results for: ANT;

Showing: 681 - 700 of 889 results

  1. Meet the New Addgene Board Members!

    Type
    Blog Post
    Published
    April 28, 2020, 1:14 p.m.
    ...teenager in high school, I knew that this is what I wanted to do,” Stegmeier says He began this journey in...wealth of resources on Addgene’s website and blog important. “The experience from the scientific community... either through direct industry collaborations, grants, or setting up commercial and nonprofit companies...

  2. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

    Type
    Blog Post
    Published
    April 23, 2020, 1:15 p.m.
    ... of the main tenets of our quality control (QC) mantra is to do our utmost to ensure that scientists are... containing just the expression cassette that we want, it also includes a small amount of spurious DNA...packaging plasmids. We decided to use this to our advantage by designing a Python script that looks for pre-defined...

  3. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    Published
    May 16, 2018, 1 p.m.
    ...be it an organic dye or FP) is one of the most important steps in the design of an imaging experiment.  ...Goedhart on the Addgene blog – outlining various important considerations when choosing a FP, but much of...valid email address).  One may also simply attach important references that serve to further characterize ...

  4. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type
    Blog Post
    Published
    Sept. 19, 2017, 1:20 p.m.
    ...xOtH5bs4HiI Advantages of MG-2I-dL5** over other photosensitizers One of the main advantages of MG-2I-dL5... Have you ever wanted to selectively kill a subset of cells in your model system? Turns out that with...

  5. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    Published
    Dec. 6, 2016, 3:30 p.m.
    ... worthwhile. Particularly if in the end all they want to do is to quickly generate a single cell line ...." If you ever run out of ready-to-use virus and want to make new lentiviral preps on your own, fear not...excited to provide this new service for researchers wanting an easier way to use CRISPR and would love to hear...

  6. DAP arrays for multiplex gene editing

    Type
    Blog Post
    Published
    July 7, 2022, 1:15 p.m.
    ...CRISPR tools, without compromising their strategic advantages in gene editing, while keeping the system to ... array with a given tRNA (derived from human or plant source), by tuning the length of 5’ leader sequences...outperformed other tRNA candidates (either from human or plant sources) in both the strength as a promoter and ...

  7. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type
    Blog Post
    Published
    April 28, 2021, 1:15 p.m.
    ...screen and transform your work into a paper or a grant. Here’s a list of tools and resources that might...differences between these two citation managers. If you want more tips on finding and organizing papers, check...post: Early Career Researcher Toolbox: Finding Relevant Papers. These are only a few of the many writing...

  8. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    Published
    Aug. 4, 2020, 1:15 p.m.
    ...SARS-CoV-2 were developed for broad use and are now important for accelerating research into this virus. One...scientific community. Addgene was awarded a Fast Grant to ensure that the scientific community will continue...Deposits of new reagents, policies from journals and granting agencies requiring materials to be deposited into...

  10. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    Published
    Sept. 28, 2016, 2:35 p.m.
    ...be of secondary importance (or tertiary, or non-existant). On the very first night of the iPSC symposium... the stem cell community for addressing these important questions that the scientific method cannot address... it took the joint effort of five post-docs to implant human kidney progenitors into a pig. In stark contrast...

  11. Management for Scientists: Delegating is Key

    Type
    Blog Post
    Published
    Feb. 20, 2015, 8:30 p.m.
    ...get more done. There are also a host of other advantages to practicing effective delegation skills: ...responsibility if things go wrong. Here are some important criteria for delegating tasks appropriately: ... and accountability? What types of issues do you want to know about right away? Provide adequate support...

  12. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    Published
    Dec. 18, 2014, 4:06 p.m.
    ...understanding of the topic will give you some advantage over other candidates. Write about your transferable...research. For example, did you help put together a grant application? Are you familiar with the publishing...whether to stay in academia or not, it is even more important to start early. Think about your plan for the ...

  13. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    Published
    Dec. 11, 2018, 12:46 p.m.
    ...handle, but I wasn’t using them to my professional advantage. It wasn’t until I wrote a blog post about a research...start using social media to their professional advantage, I’ve selected five resources that have tips for...can fit into a researcher’s work day. It’s also important to remember that it’s not the amount of time you...

  14. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type
    Blog Post
    Published
    May 3, 2018, 1:48 p.m.
    ...immunogenicity. But these studies also identified other advantages, including Cas13d-specific regulatory proteins... makes WYL proteins an intriguing counterpart to anti-CRISPR proteins that negatively modulate the activity...efficiency; where shRNA treatment produced 500-900 significant off-targets, CasRx displayed zero. Unlike Cas9...

  15. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    Published
    June 29, 2017, 1:06 p.m.
    ... a variety of common plasmid features such as antibiotic resistance genes, selectable markers, promoters... files Once you’ve identified a plasmid that you want to learn more about, updates to our sequence analyzer...used to indicate them on the map, direction (if relevant), and types. Types listed are standard GenBank...

  16. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ...  Multiplexing Method Delivery Method Advantages Disadvantages References Yamamoto Lab Golden Gate ...Multiplexing crRNA expression with Cpf1  The key advantage of multiplexing crRNA expression with Cpf1 is ...

  17. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    Published
    March 23, 2017, 2:30 p.m.
    ...self-supporting service so that Addgene is not dependent on grants. Aiming to operate under a similar model for our...word out about this new resource and providing fantastically useful educational content on viral vectors.... Addgene’s Legal Team acquired permission from relevant plasmid depositors and their institutions to distribute...

  18. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ...The lab additionally recommends using an anti-mCherry antibody rather than endogenous mCherry fluorescence...transduction with this virus. While various Cas9 mutants have been engineered to perform unique editing ...

  20. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    Published
    Sept. 26, 2024, 1:15 p.m.
    ...cloning: A SLIC makeover In 2007, LIC received an important update, courtesy of Addgene depositor Stephen ...structures at the ends of fragments. The major advantage of SLIC over Gibson assembly is cost, as T4 polymerase...homologous recombination in vitro to generate recombinant DNA via SLIC. Li MZ, Elledge SJ. Nat Methods....
Showing: 681 - 700 of 889 results