Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 898 results for: CAN

Showing: 681 - 700 of 898 results

  1. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    Published
    Dec. 18, 2014, 4:06 p.m.
    ...potential employer that you are the right candidate for the job. How can you incorporate the skills developed... school into your resume? How can you stand out among all the candidates applying for the position?   ... a Communications Coordinator. Wondering how you can develop more transferable skills? See Developing ...surprised to learn how often I see resumes from good candidates that I almost missed because I had to “dig” hard...topic will give you some advantage over other candidates. Write about your transferable skills Some of...

  2. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    Published
    Aug. 21, 2014, 3:15 p.m.
    ...careers outside of academia, sales and marketing can be great places to start. Depending on what type ...set up an ELISA kit company on whose website you can find some great information on ELISA troubleshooting...troubleshooting, ELISA kits and cell death assays. You can find Seán on twitter @Macfhearraigh.    Read more posts...background of the applicant. However, although this position did look for an academic candidate, I knew they... look for work outside of academia, a job as a ‘Cancer Marketing Coordinator’ came up at Abcam. Unlike...specifically looked for a researcher with 3 years+ of cancer research experience, a PhD or a Master’s degree...they would look for a candidate with extra experience or a marketing interest that would be able to apply...

  3. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...these tools were developed and potential ways you can use it in your research. RNA editing advantages RNA...optimize cytidine deaminase activity. While RESCUE can perform C-to-U edits, the authors note that it retains...expands the RNA editing toolkit and sequences that can be edited.   As discussed above, the ability to edit...therapeutic uses We are also curious if REPAIR and RESCUE can function in a multiplexed manner to edit multiple...HNH domains responsible for DNA cleavage, so they cannot directly edit the genome. A Cas13-based RNA editing...

  4. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type
    Blog Post
    Published
    July 2, 2020, 12:40 p.m.
    ...sequences that, when bound by transcription factors, can increase a gene’s transcription. Sometimes enhancers.... Cross-links are reversed so that DNA fragments can be detected by PCR or deep sequencing. A drawback... interactions networks as a social network. They can tell you things like which enhancers are interacting... accessibility, and epigenetic modifications, we can start to build a timeline of events that surround...

  5. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...ideal target. It is an easily accessible organ that can be monitored non-invasively over time. Importantly...an immune privileged compartment, meaning that it can tolerate the presence of antigens (such as viral ...identified serotype and the one used in Luxturna™, can efficiently transduce various types of retinal neurons... success so far. Luckily for patients, this gene can be targeted to RPE cells using a lentiviral vector...most - which means that a gene larger than 3 kb cannot be delivered using this vector. For example, although...results in preclinical studies some potential candidates for rare to ultra-rare ocular diseases are being... co-founded a non-profit to help push forward candidate therapies for these diseases. Riding on the early...

  6. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

    Type
    Blog Post
    Published
    Oct. 11, 2018, 12:30 p.m.
    ...transformants. To compensate for this, researchers can plate larger volumes of the outgrowth containing ...ml outgrowth spread on each transformation plate can be manipulated to result in appropriate levels of...assembly, ligation of mismatched pairs of overhangs can lead to incorrect assemblies, so care must be taken...confirm the accuracy of assembly. The fidelity data can be applied to derive similar high-fidelity overhangs... by guest bloggers Becky Kucera, M.Sc. and Eric Cantor, Ph.D. from New England Biolabs. Golden gate assembly...-Crick base pair substrates, it demonstrates significant activity on some mismatch-containing pairings...our guest bloggers Becky Kucera, M.Sc. and Eric Cantor, Ph.D. from New England Biolabs. Becky Kucera is...

  7. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...adeno-associated viral vectors can also target non-dividing cells, lentiviral vectors can carry much more genetic... often used as a substitute for HIV-1 Env, which can only infect CD4+ cells. 3rd generation lentiviral...Lentiviral vectors in the clinic Since lentiviral vectors can deliver a large amount of DNA (~8 kb) with a relatively...beta-thalassemia. Lentiviral vectors are promising agents for cancer immunotherapy, but this research is still in the...Central PMCID: PMC3355472. 8. Lentiviral vectors in cancer immunotherapy. Oldham RA, Berinstein EM, Medin ...

  8. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...essential to most bacteria, custom CRISPR antimicrobials can target specific sequences in a single virulent bacterial...plasmids containing a phage origin of replication that can be packaged into replication-incompetent phage particles...Once these bacteria have been killed, antibiotics can be used to target the sensitive population. Yosef.... Future research will show if this new strategy can help us win back ground in the fight against bacterial...

  9. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    Published
    Aug. 20, 2015, 2:30 p.m.
    ...federal regulations. A grant professional’s job duties can range from coordinating the grant application and...completed. To enter and advance the profession, you can find professional development, certification, degrees...the grants profession? The four steps listed below can give you the resources and path to help you enter... first career, a mentor in the Grants Profession can help you focus your career goals, identify your niche...evaluates a candidate's expertise (a minimum of 3 years experience) and experience through the candidate application... the candidate’s ability to write a grant application through a written exam, and the candidate’s knowledge...grant seekers to use online applications with significant character limits for the written portion of the...

  10. PEI Calculator for Planning AAV Packaging Transfections

    Type
    Blog Post
    Published
    Jan. 23, 2018, 2:04 p.m.
    ... Preparation is a key step to any experiment and can help prevent future headaches. To help you plan PEI...you need to do a few calculations. This calculator can do the math for you and it’s simple to make adjustments...

  11. Countdown to Halloween @Addgene

    Type
    Blog Post
    Published
    Oct. 29, 2014, 5:26 p.m.
    ...there’s always nostalgia (old board games and movies can provide great character costumes). All of this costume... chose their favorite plasmid and replicated it. Can you guess each plasmid? Our lab paid tribute to ...Success is sweet – as sweet as all that Halloween candy you’ll gorge yourself on in the coming days. Hopefully...

  12. Addgene Depositors Get More Citations

    Type
    Blog Post
    Published
    April 20, 2017, 2:30 p.m.
    ...Addgene (Figure 1). Even aggregated this much, you can see the 2013 ‘bump’ related to the CRISPR/Cas papers...comprised 10 percent. Based on this new re-weighting, we can compare citations where an equal share of papers ...Microbiology  1.6% 1.2%  0.75   125.3 In figure 2, you can see the full impact from using these weighted numbers...journal. And so, while our quantitative analysis cannot rule out all other explanations, we do think that...used in this work. Samantha Zyontz is a Ph.D. candidate at the MIT Sloan School of Management. She is ...

  13. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    Published
    Aug. 23, 2022, 1:15 p.m.
    ...issues creates conditions under which misinformation can flourish. I’ve never forgot about all the anxious...helped run workshops where I showed people how they can make reusable cloth menstrual pads while we talked... the very least, believe – to be true so that we can tailor our communications to better meet the needs...controversial medical issues. Participants gave significantly more favourable ratings to the abstracts that...understand. Why should we trust something that we cannot read or verify for ourselves? Further, when science...

  14. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    Published
    April 4, 2023, 1 p.m.
    ...aerobe. It’s also a methylotropic yeast, meaning it can utilize methanol as its sole source of carbon and...industries as a protein expression workhorse. This yeast can also give more protein bang for your media buck -...media change into methanol and peroxisomal autophagy can be induced by removing methanol from the media. This...

  15. AAV Packaged on Request is Here!

    Type
    Blog Post
    Published
    Jan. 10, 2025, 1:01 p.m.
    ...service, has officially launched! As of today, you can request any of over 3,000 eligible AAV cargo plasmids...Packaged on Request With Packaged on Request, you can find your plasmids and your viral packaging service...

  16. Editor's Choice, October 2016

    Type
    Blog Post
    Published
    Nov. 4, 2016, 2:30 p.m.
    ...number of postdocs various academic institutions can afford to hire. It will be difficult for some of ...establishing a thriving graduate student community can make the ups and downs of graduate school much easier...

  17. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    Published
    Aug. 20, 2020, 2:30 p.m.
    ...zebrafish embryos, and they can be used to modify genomic loci that cannot be modified using wild-type...Nov 12, 2015 and last updated Aug 20, 2020. Cas9 can be used to modify any desired genomic target provided...Cas9 variants. These variants, named SpG and SpRY, can target NGN PAMs and NRN and NYN PAMs, respectively...) is about 1 kilobase smaller than SpCas9, so it can be packaged into adeno-associated virus (AAV).  At... D1135E variant is far more selective for the canonical S. pyogenes PAM sequence NGG compared to wild-...

  18. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ...might affect your work, and how understanding it can be used for good.  First, why are plasmids incompatible...replication. However, in iteron-containing plasmids,  RepA can also bind to its own promoter and inhibit its own...., 2015).  It is also worth noting that plasmids can be symmetrically incompatible, meaning that both ...the other, usually a smaller, higher copy plasmid can more effectively titrate replication or partitioning... of replication, the negative regulation system cannot distinguish between the different plasmids. The...to an identification issue: the CBPs in the cell can’t tell the difference between plasmids if they have...

  19. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    Published
    March 27, 2018, 1:32 p.m.
    ...this post we'll walk through a variety of ways you can use AAVs to improve your genome editing experiments...pAAV-SEPT-Acceptor and pAAV-TK-Acceptor) so that they can be easily adapted to edit your gene of choice. These...CRISPR components as ribonucleoproteins (RNPs) that can be supplied separately from the viral vector. This... a variety of cell types, now you know that AAVs can provide a powerful boost to genome editing experiments...AAV gene editing to introduce naturally occurring cancer-causing mutations into the endogenous allele of...Professor of Oncology at the Lombardi Comprehensive Cancer Center, Georgetown School of Medicine. His research...consequences of cohesin gene inactivation in human cancer.     References 1. Russell, David W., and Roll ...

  20. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ... kb in length. Human adenovirus type 5 (hAdV-C5) can be considered the prototype, although many viruses...available by this deletion from the genomic plasmid can be used to house a recombinant sequence (transgene...potentially because some of the wild-type viruses can persist as a latent infection in certain tissue reservoirs...using vectors, and immune inactivation of the vector can still occur even in the case of no previous immunity...basic research, like other viral vectors, though significant challenges remain (Wold & Toth, 2013).  While...Adenovirus Vectors for Gene Therapy, Vaccination and Cancer Gene Therapy. Curr Gene Ther, 13(6), 421–433. https...
Showing: 681 - 700 of 898 results