We narrowed to 834 results for: CAT;
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TypeBlog PostPublishedDec. 6, 2016, 3:30 p.m....represent the fraction of that total with the indicated function. One huge reason CRISPR has become such...achieving the gRNA representation recommended in the publications associated with these libraries. Please note...
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How to Lead a Great Meeting
TypeBlog PostPublishedFeb. 9, 2016, 3:30 p.m.... dialogue and community among participants, clarification - new and useful information, and invigoration...be the representative in the meeting and then communicate outcomes to the rest of their team. Here's a ... -
Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis
TypeBlog PostPublishedAug. 28, 2014, 3:34 p.m.... I hope these tips demonstrate that plasmid verification is not just necessary but also an easy process.... Please vist Addgene's resource for plasmid verification to find additional tips and detailed protocols... -
Antibodies 101: Flow Cytometry Controls
TypeBlog PostPublishedJune 6, 2024, 1:50 p.m....ll need a few controls specific to flow as an application. These controls will allow you to distinguish...commercial antibody pages. Flow cytometry can be a complicated and daunting process. Controls like single color... -
Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies
TypeBlog PostPublishedJuly 28, 2017, 1:30 p.m....beforehand on which chromosome your docking site is located). This amounts to a small difference of two or ...throughout the lifespan of the fly to determine the implications of your genetic manipulations. You can produce... -
Multiplex Genome Editing with CRISPR-Cpf1
TypeBlog PostPublishedMay 9, 2017, 2:12 p.m.... a simple and effective method with multiple applications (in vitro, in vivo, transfection, viral transduction...Resources Find CRISPR Plasmids for Your Research Catch up on Your CRISPR Background with Our Guide Pages... -
Antibodies 101: Isotypes
TypeBlog PostPublishedOct. 19, 2021, 1:15 p.m.... Isotype controls are used in antibody-based applications including flow cytometry, immunohistochemistry...of these five isotypes, such as the number and location of disulfide bonds, the number of constant domains... -
Top Requested Lentivirus and AAV of 2016
TypeBlog PostPublishedJan. 6, 2017, 3:56 p.m.... preps. Cas9 is the headliner of most CRISPR applications and can be expressed from your cell line of ...Find Ready-to-Use Lentivirus Find Ready-to-Use AAV Catch up on Chemogenetics ... -
Plasmids 101: Blue-white Screening
TypeBlog PostPublishedJune 4, 2015, 1:03 p.m....insert. All colonies on this plate should be blue, indicating that your IPTG and x-gal are working as they ...of false positives: Blue-white screening only indicates the presence of AN insert, not necessarily YOUR... -
Will You Be My Mentor? Finding and Asking for Mentoring Support
TypeBlog PostPublishedFeb. 4, 2014, 3:50 p.m....great way to get started with mentoring. Read this Catalyst Report “Making Mentoring Work” to learn about ...tapping into it and helping make it work by being an educated mentee. Other opportunities: Try virtual mentoring... -
All in a Twist: dsRNA
TypeBlog PostPublishedFeb. 2, 2023, 2:15 p.m....generate dsRNA as part of their infection and/or replication process within the host. dsRNA viruses are inherently...generate dsRNA intermediates as part of the viral replication process. dsDNA viruses yield dsRNA through a ... -
Rosella: A Fluorescent pH-Biosensor for Studying Autophagy
TypeBlog PostPublishedApril 13, 2017, 2:30 p.m....radioactivity in the culture supernatant (this is indicative of protein degradation). To control for protein...studying autophagy. This method relies on the identification of autophagic structures based on morphology... -
Transferable Skills Guide: Problem Solving
TypeBlog PostPublishedNov. 30, 2021, 2:15 p.m....might even be expected to write up a report to communicate your findings to your colleagues. So perhaps ...about the issue, assessed potential protocol modifications, and ultimately decided on the changes to implement... -
What's New in CRISPR - November 2021
TypeBlog PostPublishedNov. 18, 2021, 2:15 p.m....AsCas12f1. They found that this system, without any modifications, is effective in genome editing in both bacteria...therapeutically relevant gene targets and expand the application to the possible treatment of genetic disease.... -
Seven Tips for Using LinkedIn as a Scientist
TypeBlog PostPublishedOct. 1, 2019, 1:21 p.m.... example, I will often see a whole bunch of notifications “a postdoc from this Uni viewed your profile...look straight at the camera. Pro tip: Turn off notifications to your network of profile changes. If you are... -
Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics
TypeBlog PostPublishedNov. 3, 2020, 2:15 p.m....tegmental area (VTA) of the brain. Since the VTA is located in the midbrain, fiber-optic cannulas were still...right secondary motor cortex (M2). Since M2 is located on the surface of the brain, to deliver light to... -
Reproducibility for Everyone: Lessons from an Open Science Collaboration
TypeBlog PostPublishedFeb. 28, 2019, 1:34 p.m....mini-grant, which will fund a project to support a dedicated website to host all the materials in one place...for me was to collaborate with this incredibly dedicated team. Many thanks to Tyler Ford, Caroline LaManna... -
Developing Lab Management Software for Biology
TypeBlog PostPublishedAug. 25, 2016, 2:30 p.m....for a scaleable lab. When you need to track the location, quality, growth, and legal status of thousands...stab culture Ship the stab culture to the proper location To keep ourselves from mixing up samples and ... -
Gendered Innovations: Why Does Sex of the Cell Matter?
TypeBlog PostPublishedJuly 21, 2016, 2:30 p.m..... To remedy this, Shah et al. provide a table indicating the sex of the most commonly used cell lines ... work to be done! Researchers need to learn sophisticated methods of sex and gender analysis. Universities... -
Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing
TypeBlog PostPublishedMay 5, 2015, 3:54 p.m....looking for a particular protocol or are trying to replicate an experiment, the methods section probably won...is science’s way of validating work prior to publication, it doesn’t mean that there are no mistakes or...