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... that we once again reached new heights on the Addgene blog: we surpassed 60,000 views for the month of... would love to see more posts like this on the Addgene blog. Read the post for the full details and many...,” was contributed by Kurt Swanson, husband of Addgene Director of Biology, Lianna Swanson, and researcher...of therapeutics. It also represents a first for Addgene - while we often participate in 5Ks throughout ...Letter Multiple Plasmids at a Low Price: Kits from Addgene Savvy Advocates Needed to Navigate a Scientific...Through Chemistry & Small Molecule Fluorophores Addgene's Cancer Collection Pages Connect the Research Community...Francisco and would like to give my thanks to all the Addgene supporters who spoke with me and deposited plasmids...

...communicating science to the general public and training the next generation of science communicators. In...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional... is a source of scientific information for the general public as well as a platform for scientists to ...discuss the importance of actionability on the Addgene blog and how SITN short-form blog posts help readers...Additional Resources on the Addgene Blog Resume Writing for Non-academic Science Careers 10 Steps to a Perfect...

...author do not represent a formal stance taken by Addgene or its staff. In Washington, D.C. as well as sister... in education, government, and civilization in general (1). Science and politics? Science is an apolitical...as evidence of climate change, the safety of genetically modified organisms (GMOs), the absence of a link... human consumption in comparison to 37% of the general public (5, 6). Similarly 90% of active research...mostly due to human activity while much less of the general public agrees (5, 6). Many of the new administration...them. Other popular reasons to march include: General inclusion of science to inform policy (17). The...relatable (17, 20). This is an effort to show the general public that science is done by people like them...

...viral vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols...associated with the brain’s reward circuits are generally linked to dopamine (DA), a well-known reward chemical...in freely moving mice Record DA dynamics of optogenetically activated and inhibited neurons in live mice...neuronal imaging of dopamine dynamics with designed genetically encoded sensors." Science 360.6396 (2018): eaat4422... PMCID: PMC6287765. Sun, Fangmiao, et al. "A genetically encoded fluorescent sensor enables rapid and ...PMCID: PMC6092020. Additional resources on the Addgene blog Read all of our viral vectors blog posts ...

...PMC5517107. Additional resources on the Addgene blog Subscribe to the Addgene blog Find lab tips for your research...familiar with the alarming amount of waste that is generated. This is a systemic problem that we need to address...to learn about practices I could bring both to Addgene, and share with my peers. In fact, there was so...account for an estimated 2% of global plastic generation. Less than 9% of plastics are ever recycled, ... about conservation in the lab Resources on Addgene.org Sign up for our quarterly newsletter Help other...resources by sharing plasmids Find plasmids at Addgene ...

...options Resources on Addgene.org Check out Addgene's job openings Find eBooks from Addgene, including the ...development tips, take a look at the Addgene Science Career Guide eBook Addgene Science Career Guide eBook.   ...own research project.  3. Do you prefer being a generalist? Working on a variety of projects and in different...   Resources Resources on the Addgene blog Learn about career planning resources What Do I Do Now? ...

...include: Optogenetics AAV Control AAV Biosensor AAV And more! Optogenetics Optogenetics continues ...viral vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols...you pick the best AAV for your experiment. The Addgene viral service has now expanded to offer many of...pEF1a-DIO-FLPo-WPRE-hGHpA    Additional resources on the Addgene blog Read all of our viral vectors blog posts ...

...Immunofluorescence Resources on Addgene.org Find recombinant antibodies available at Addgene Protocols for Immunocytochemistry...antibodies and appropriate secondary antibodies. The general process is similar to standard immunofluorescence...IHC validated or ICC validated antibodies from Addgene), or be prepared to run some extra controls and...quantify accurately, but can be especially helpful to generate more signal from low abundance targets. Analysis...you find yourself stuck with red-green images generated automatically from the microscope software, you...three targets or you’re pushing the limits like Addgene depositor Ronald Germain (Radtke et al., 2020 multiplexed...10.1073/pnas.2018488117 Additional Resources on the Addgene blog Introduction to Antibodies Which Fluorescence...

...study apoptosis in vivo, scientists have used genetically encoded fluorescent executioner caspase reporters...to issues with tissue autofluorescence, cell heterogeneity, and/or cell position changes (To et al., 2016...They then optimized the length of both linkers to generate a reporter that would fluoresce: FlipCherry. FlipCherry...array of diseases and pathologies such as neurodegenerative diseases, autoimmune disorders, and cancer...find the plasmids for FlipGFP and FlipCherry at Addgene. References Cabantous, Stéphanie, et al. "A new...PubMed PMID: 30821975. Additional resources on the Addgene blog Read our fluorescent proteins blog posts ...Read our plasmids 101 blog series Resources on Addgene.org Browse the fluorescent protein collection Find...

...exciting revelation. Science in any field can generally be applied to improve our lives and solve global...Typically has a Master's or Bachelor's degree Generally a short-term position (1-2 years) Often a gap-...who opted to join labs in cancer biology or neurodegenerative disease because they had close relatives suffering...that can make or break a lab experience. Previous Addgene blogs have discussed the process that goes into...also found that it is more common among first generation students, women, BIPOC, and other minoritized...academia or industry, so feel free to explore! Addgene has an entire page dedicated to different career... the Daily Psychological Experiences of First-Generation College Students. Social Psychological and Personality...

...resources on the Addgene blog Addgene Blog Biosensors posts Resources on Addgene.org Fluorescent Biosensors...where should you begin? Have no fear, Addgene is here to help!  General Design Figure 1: Two proteins...the FP back together (Figure 1). This method is generally known as Bimolecular Fluorescence Complementation...proteins in Caenorhabditis elegans. Goudeau et al. Genetics. 2021 Cynthia Kenyon mRuby4 mRuby4(1–10), ...

...Toolbox for quickly generating zebrafish transgenic lines to study neurodegenerative diseases. Listen to...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...Additional Resources on the Addgene Blog Listen to Our Podcast Interview with Eye Researcher Connie Cepko...Science Videos from Tom McFadden Resources at Addgene.org Sign up to Receive Our Newsletter Check Out ...

...disponible en español! Esta traducción fue regalada a Addgene por Daniel Mejías Sánchez, ¡y no podríamos estar...emocionados! Después de recibir la traducción, Addgene contrató a una traductora científica profesional...Descargar CRISPR 101: Una herramienta de edición Addgene tiene una gran audiencia internacional, incluido...available in Spanish!  This translation was gifted to Addgene by Daniel Mejías Sánchez, and we could not be more...more excited! After receiving the translation, Addgene contracted with a professional scientific translator...Download CRISPR 101: Una herramienta de edición  Addgene has a large international audience, including working...

...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.      Additional... In this episode of the Addgene Podcast, we introduce you to the Journal of Emerging Investigators, an...recently submitted to the journal with funding from Addgene. Listen to the interview with the Journal of Emerging...Additional Resources from the Addgene Blog Find education resources Use our Plasmids 101 eBook to teach ...genome enegineering technology Resources at Addgene.org Find basic molecular biology protocols Check...

...kit to use?  Obtaining higher quantities of DNA generally means starting with higher volumes of bacterial...reduce your lab’s environmental impact, check out Addgene’s blog post on bringing sustainable practices for...Monarch®, QIAGEN’s QIAwave, and Milllipore Sigma’s GenElute™ kits were all specifically designed with environmental...Series NEB Monarch QIAGEN QIAwave Millipore Sigma GenElute Reduced waste from kit materials Reduced plastic...relatively little work after the initial setup. (Addgene actually uses some of these — if you’re curious... (These are mostly miniprep kits! Larger kits generally take longer.) Make sure to take a look at the...Nyla Naim. Resources Additional resources on the Addgene blog Plasmid Preps: Different Purity, Different...

...intern who shared their experience working at Addgene. An Addgene Summer Internship Matteo Tardelli, who ...contributing to the Addgene blog? We'd love to hear from you! Email us anytime at news@addgene.org. ... very thankful time of year for those of us at Addgene! And we can’t think of any better way to start ...to the blog. Thank you! More resources on the Addgene blog 10 Great Guest Posts We're Thankful For Lucky...

...molecular weight ladders were recently deposited with Addgene. A team of undergraduate students led by Dr. Song...restriction digested with PstI or EcoRV, these plasmids generate 100 bp or 1 kb DNA ladders, respectively. Unlike...pPSU1 and the pPSU2 plasmids are available from Addgene. Learn How to Analyze a Restriction Digest References.... L., & Tan, S. (2017). The pPSU Plasmids for Generating DNA Molecular Weight Markers. Scientific Reports...Central PMCID: 5446395. Additional Resources on the Addgene Blog Are you a Frugal Scientist? Save Money with...the Evolution of Lab Techniques Resources on Addgene.org Use the Kit-Free Alkaline Lysis Plasmid Miniprep...

...production requires animal sacrifice and precludes genetic engineering of a tag or fusion with a reporter ...described in Pleiner et al, the key steps for generating nanobodies are outlined below; no alpaca required...your work. Species Antibody Subclass Nanobody Addgene Plasmid Rabbit N/A* TP897 pTP1183 (1x cysteine...nanobodies from the column. bdNEDP1 protease can be generated using plasmid pDG02583. Label the Nanobodies: ...29263082 Find plasmids from this publication at Addgene. 2. Bates, M., Böhning, M.I., Chug, H., Görlich...PubMed PMID: 23495938. Additional Resources on the Addgene Blog DIY DNA ladders from Penn State University... protein expression Additional Resources on Addgene.org Find more nanobody expression plasmids here ...

...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...Plasmid Rap Plasmid rap video made with support from Addgene.      Check out more science education tools...looking for creative ways to implement the next generation science standards and communicate science. Learn...Additional Resources on the Addgene Blog Get Advice on How to Build a Grad Student Community Check out All...Plasmids 101 Series Additional Resources on Addgene.org Check out Our Protocol Pages for New Protocol...

... Sign up to write for the Addgene blog! To sign up to write for the Addgene blog simply email us. We can...write for us! Click Here to Contribute to the Addgene Blog Possible guest topics Some topics you might...Exposure - With over 100,000 views each month, the Addgene Blog is a great way to get your name out there!...include on CVs and Resumes. An Expanded Network - Addgene will promote your blog on a variety of social media...applying CRISPR for mouse genome editing. Post: Generating Mouse Models Using CRISPR/Cas9   Erik L. Snapp...