We narrowed to 738 results for: HA
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TypeBlog PostPublishedDec. 12, 2013, 3:35 p.m....promises and associated sequence information. Addgene has partnered with the BioBricks Foundation to distribute...
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Designing Your Chalk Talk for the Academic Job Interview
TypeBlog PostPublishedMarch 12, 2019, 1:37 p.m....experiments or a laundry list of molecules to screen has unclear utility. You cannot predict what will happen... -
Great Results Start with Great Standard Curves
TypeBlog PostPublishedAug. 15, 2024, 1:15 p.m....sample, called a standard. The ideal standard curve has at least five dilutions, with each step of the series... -
Antibodies 101: The Four ELISAs and When to Use Them
TypeBlog PostPublishedJuly 25, 2024, 1:15 p.m....While the method is simple and saves time, it also has several drawbacks. Direct ELISAs generally have higher... -
Viral Vectors 101: Producing Your rAAV
TypeBlog PostPublishedJuly 16, 2024, 1:15 p.m....titer on every qPCR run. Note that the rAAV sample has to be digested with DNase before undergoing titering... -
PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs
TypeBlog PostPublishedJune 25, 2024, 1:15 p.m....transcription template (RTT). The design of the pegRNA has been shown to play a critical role in the editing... -
INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons
TypeBlog PostPublishedDec. 7, 2020, 2:53 p.m....technology potential enabled by CRISPR-transposons has yet to be fully realized. Many thanks to our guest... -
Hot Plasmids - August 2020
TypeBlog PostPublishedAug. 18, 2020, 1:15 p.m....with low affinity variants of TEV. The new system has been demonstrated to work for evolution of other ... -
Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR
TypeBlog PostPublishedJuly 2, 2020, 12:40 p.m.... original CAPTURE method is that a new cell line has to be created for each genomic loci of interest. ... -
Viral Vectors 101: The Retroviral Lifecycle
TypeBlog PostPublishedJuly 27, 2023, 1:15 p.m....Noninfectious ARK mouse embryo cell lines in which each cell has the capacity to be activated to produce infectious... -
Course-Based Undergraduate Research Experiences (CUREs)
TypeBlog PostPublishedFeb. 9, 2023, 2:15 p.m.... from R1 institutions to community colleges, and has helped provide original research experiences for ... -
Negative Can Be Positive: Open AAV Data with Addgene
TypeBlog PostPublishedSept. 6, 2022, 1:15 p.m....transduced by each serotype. Working at Addgene has shown me this was far from an isolated case. Our ... -
Bioinformatics at Addgene
TypeBlog PostPublishedJuly 14, 2022, 1:15 p.m.... and continuous development of in-house software has been crucial for us to keep pace with our current... -
Design Tips for Prime Editing
TypeBlog PostPublishedJan. 23, 2025, 2:15 p.m....prevents the prime editor from re-engaging with DNA it has already edited. Created with BioRender.com. ... -
An Inside Look into Addgene's Dev Team
TypeBlog PostPublishedJan. 23, 2020, 2:45 p.m....There are many moving parts to our software, which has grown with the company. It would be easy to assign... -
Plasmids 101: Choosing an Antibiotic Resistance Gene
TypeBlog PostPublishedOct. 10, 2023, 1 p.m....since bacteria won’t divide immediately after it has been heat-shocked or electrically zapped. As a result... -
How to Be an Excellent Trainee
TypeBlog PostPublishedSept. 26, 2023, 1:15 p.m....the bench waiting for you, prep for research work has to be done by the researchers themselves. Be ... -
Bacterial Toxin-antitoxin Systems as Molecular Biology Tools
TypeBlog PostPublishedNov. 1, 2018, 12:35 p.m....two flanking repeats. In cells where recombination has not occured, methanol induces the production of the... -
Tips for a 1st time AAV user (by a Rookie AAV user)
TypeBlog PostPublishedOct. 23, 2018, 11:49 a.m....how to create the iodixanol gradient layers, and has some great pointers that while help you master iodixanol... -
Simplify Cloning with in vivo Assembly
TypeBlog PostPublishedOct. 18, 2018, 12:37 p.m...., TOP10, and Mach1. No common lab cloning strain has failed so far. What is most important is the transformation...