We narrowed to 868 results for: REN;

...comparison to each other? The gRNAs must target different strands to create a DSB, but this can be accomplished...Shuqi Yan designed experiments to test nickase preferences using HEK293 cells. They started by comparing...

...some our most popular CRISPR blogs to keep them current. Here are some of the changes we’ve made: New CRISPR...already has five blue flame plasmids covering three different epigenetic effectors. The diversity of tools is...

...SunTag? Tanenbaum et al. performed a variety of different experiments in their paper, and so can you! In...we’ll save that application for another day! References 1. Tanenbaum, Marvin E., et al. “A protein-tagging...

...standards. Bricking science reminded us of our inner children who have no preconceived obstacles, optimistically...potential, and who sometimes wish they could live differently than we do day to day. We discovered that it ...

...meiosis in biology. Lots of it is researching the different aspects of the subject you're writing and then...developing new online courses about unique topics that aren’t already offered by eScience Labs.  “We work with...

... just find one that naturally occurs? This is different than the engineered approach used to make eSpCas9...are available from Addgene. Happy CRISPRing! References Harrington, Lucas, et al. "A thermostable Cas9...

...includes an antibiotic resistance cassette and you aren’t introducing an additional antibiotic resistance...plasmids at Addgene Read our molecular biology reference Watch molecular biology protocol videos ...

...nontarget strand. Protein Cas: Different CRISPR systems involve different proteins, but many of them are...

...strain or a mutated version of YGOI to test the different phenotypes of the specific mutations. For these..., or knocking down YGOI through RNA-mediated interference (e.g. L4440) and observing the downstream effects...

...likely happening in your community, and, if they aren’t, think about launching your own. Close the gap ...anyone looking to make their science matter. If you aren’t ready for a training or one isn’t available yet...

...research project “entails good judgement with the current technology to identify a problem, asking what approach...will I take and what can I learn, learning the difference between good and bad data, and knowing how to...

...how to prepare antibiotic stocks, see Addgene's Reference Page. How else can antibiotics be used in the ... on Addgene.org Browse Our Molecular Biology Reference Pages View our Plasmid Protocols Find Your Perfect...

...moieties carry out these functions using slightly different Cas proteins and PAM sequences. A total of eight...involves CRISPR/Cas technology? Let us know.  References Ishino Y, Hideo, S, Makino K, Mitsuko A, Nakata...

...experimental design and usage of these tools, which aren’t always easy to find in publications, so this is...submit data. Please note that the data hub is currently still in beta version, so we’re always looking...

... containing an anti-GFP nanobody fused with a different functional element (tyrosine sulfation consensus...APEX2), allowing for quantitative analysis using different detection methods. Since the anti-GFP nanobody...

...studying animals than plants, and biology teachers’ preference for using animal systems to demonstrate biological... a nominal fee for university professors, are currently available through TAIR with a new ABRC database...

...Dr. Polka made R-body mutants that respond to different pH’s and some that are fused to fluorescent proteins...your target gene, activation and repression of different genes in a single cell, and localization of a ...

...topoisomerase I (1). If a 5′ hydroxyl group from a different DNA strand comes along, it can attack this covalent...may allow you to see colonies within 8 hours. References: 1. Shuman S. "Recombination mediated by vaccinia...

...of Medicine  Baylor College of Medicine Boston Children's Hospital  Brandeis University  Broad Institute...Studies The Scripps Research Institute St. Jude Children's Research Hospital  Stony Brook University  University...

...Ready to start epitope tagging?   References and Resources References Terpe, K., Overview of tag protein...