We narrowed to 859 results for: STI

...making virus’ or what gag and pol stand for, we demystify all components of viral vectors here! Need Virus...into the host genome (more on this below), it’s still used to deliver gene expression constructs. Sandwiched...

...producing the proteins – are not, and many of the existing GCE tools are not very robust. When using GCE,...work. Plus, keep an eye on our blog – we’ll be posting more posts on the various protocols and helping...

...Image courtesy of National Human Genome Research Institute. Its genome, however, isn’t enough to ensure...their low immunogenicity, which means they do not stimulate a strong immune response when introduced into ...

...each from a constitutively active CMV promoter. Constructs were then verified by both diagnostic restriction...

...the Genetic Pertubation Platform at the Broad Institute have expanded the genetic screening toolkit by...separately. The proteins, which are purified via histidine tags, are optimized for SDS-PAGE gels and can ...

...isotype? An isotype determines several key characteristics of an antibody as well as the role it plays... regions result in several unique physical characteristics of these five isotypes, such as the number ...

...Monoclonal Versus Polyclonal Antibodies: Distinguishing Characteristics, Applications, and Information Resources...

..., integrated reporter for assaying CRISPR/Cas-stimulated HDR activity) The Madison lab created SRIRACCHA...integrated surrogate reporter for assaying CRISPR/Cas9-stimulated homology-directed repair. Journal of Biological...

...certain sites, or make only a subset of base substitutions.  The need for speed: base editing before Cas...compared to the original TadA-7.10 enzyme. After testing numerous TadA variants, they identified TadA-8e...

... from their native conditions of ~0°C, which drastically affects their stable folding and efficient functioning...beyond! Keeping in spirit with iGEM’s emphasis on holistic, community-centric science, our team has continuously...

..., B-B’, and C-C’) that give the feature its characteristic T-shape. The arrangement of the B-B’ and C-... efficiency. Our practical knowledge of ITRs is still limited, but this area of research is important ...

...promoters, are always active and thus referred to as constitutive promoters. Others are only active under specific...complexed with cAMP activator protein (CAP) can also stimulate AraC binding to I1 and I2 sites. Supplementing...

...commercial antibody against them. There’s also a question about how specific those interactions are. This... that, but the system is so flexible. Other investigators can easily adapt these vectors for use with ...

...less than an hour, thus a population of cells constitutively expressing a targeted Cas9 should possess indels...reduces breaks and mutations at off-target sites. Christina Mork contributed to the update of this post. Thank...

.... Staining with multiple HRP-RANbodies is indistinguishable since they produce the same signal, so only...fluorescent protein (i.e. GFP, RFP) Detecting GFP reconstitution across synaptic partners (GRASP) assays No...

...proteins interact or oligomerize in cells? This question is largely unanswered. Ideally, fluorescent proteins...cells (Shaner et al, 2013; Bindels et al, 2017). Still, the data presented in papers should be used with...

...Morgan, our senior engineer? The answer to these questions--which were real, not hypothetical--was clearly...Others in the meeting might also have the same question and asking can help the team identify possible...

... an electrical state. This state, known as the resting potential (Vmem), can be measured as the imbalance...fusion of the voltage sensing domain of a Ciona intestinalis voltage sensor and a mutated version of the ...

...Maybe take a deep breath?" Once you answer the question, you need only to take that step. Repeat that ... to be anxious, nervous, and/or apprehensive. I still get nervous quite regularly, and I've had multiple...

... while puzzling through your broader research questions and designing future experiments. Being able to...Scientific Method? Imagine your problem is just a question you have about the world. You might do some background...