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We narrowed to 852 results for: STI

Showing: 681 - 700 of 852 results
  1. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    Published
    Aug. 6, 2024, 1:15 p.m.
    ...prototypical gamma-retrovirus.    Frequently asked questions about γ-retroviral vectors How do engineered γ-retroviruses...post‐transcriptional regulatory element; sequence that stimulates the expression of transgenes via increased nuclear...
  2. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    Published
    June 6, 2024, 1:50 p.m.
    ...application. These controls will allow you to distinguish real results from background noise or nonspecific...positive and negative cells. This can be achieved by adjusting your voltage using your single color controls ...
  3. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    Published
    March 21, 2023, 1:15 p.m.
    ...Image courtesy of National Human Genome Research Institute. Its genome, however, isn’t enough to ensure...their low immunogenicity, which means they do not stimulate a strong immune response when introduced into ...
  4. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    Published
    June 29, 2021, 1:15 p.m.
    ...Monoclonal Versus Polyclonal Antibodies: Distinguishing Characteristics, Applications, and Information Resources...
  5. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    Published
    Jan. 28, 2020, 2:35 p.m.
    ... from their native conditions of ~0°C, which drastically affects their stable folding and efficient functioning...beyond! Keeping in spirit with iGEM’s emphasis on holistic, community-centric science, our team has continuously...
  6. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ...less than an hour, thus a population of cells constitutively expressing a targeted Cas9 should possess indels...reduces breaks and mutations at off-target sites. Christina Mork contributed to the update of this post. Thank...
  7. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    Published
    April 10, 2018, 12:56 p.m.
    .... Staining with multiple HRP-RANbodies is indistinguishable since they produce the same signal, so only...fluorescent protein (i.e. GFP, RFP) Detecting GFP reconstitution across synaptic partners (GRASP) assays No...
  8. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    Published
    June 15, 2017, 2:30 p.m.
    ...proteins interact or oligomerize in cells? This question is largely unanswered. Ideally, fluorescent proteins...cells (Shaner et al, 2013; Bindels et al, 2017). Still, the data presented in papers should be used with...
  9. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    Published
    Nov. 21, 2019, 1:53 p.m.
    ...Morgan, our senior engineer? The answer to these questions--which were real, not hypothetical--was clearly...Others in the meeting might also have the same question and asking can help the team identify possible...
  10. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    Published
    Oct. 29, 2019, 2:52 p.m.
    ... an electrical state. This state, known as the resting potential (Vmem), can be measured as the imbalance...fusion of the voltage sensing domain of a Ciona intestinalis voltage sensor and a mutated version of the ...
  11. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    Published
    Nov. 30, 2021, 2:15 p.m.
    ... while puzzling through your broader research questions and designing future experiments. Being able to...Scientific Method? Imagine your problem is just a question you have about the world. You might do some background...
  12. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...their activity in mammalian systems has been in question. Three different labs have recently published ... the prime editor, yielding PEmax, to work synergistically with epegRNAs and PE4 and PE5. These enhanced...
  13. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    Published
    July 14, 2021, 1:15 p.m.
    ...as KLH or Freund’s adjuvant is injected, which stimulates the overall immune response. Once the initial...increases the likelihood that one of the clones will still recognize your protein. For the same reason, they...
  14. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    Published
    April 20, 2023, 4 p.m.
    ...Maybe take a deep breath?" Once you answer the question, you need only to take that step. Repeat that ... to be anxious, nervous, and/or apprehensive. I still get nervous quite regularly, and I've had multiple...
  15. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...edition, we’re going to take a look at a very interesting tool that can be used for creating (excuse the...sites are 34-base-pair long recognition sequences consisting of two 13-bp long palindromic repeats separated...
  16. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    Published
    Aug. 6, 2019, 12:55 p.m.
    ...impacts the expression level of the transgene. In existing strains of mice, the transgene can be maintained...tissue-specific promoter). And, Cre may also be either constitutively expressed, or inducible (this is explained ...
Showing: 681 - 700 of 852 results