...of multiple circular DNA forms of colicinogenic factor E-1 from Proteus mirabilis. Biochemistry. 1968 ...Guttman A, Thompson L. Vaccine Plasmid Topology Monitoring by Capillary Gel Electrophoresis. Curr Mol Med... JA, Carnes AE, Hodgson CP. Plasmid DNA vaccine vector design: impact on efficacy, safety and upstream...

...modular toolkits build single plasmid targeting vectors that encode the gRNA transcript and the repair ...recognized by the 2020 Nobel Prize in Chemistry), and Victor Ambros and Gary Ruvkun for their seminal work on....114.170423 Schwartz ML, Jorgensen EM (2018) SapTrap vectors for introducing point mutations with unc-119+ selection...

...subtilis libraries. Hot Plasmid 2: Transcription factor fusions modulate iPSC reprogramming efficiency....Out All of Our Hot Plasmids Articles Browse the Repository  ...

...Now we have over 8,000 CRISPR plasmids in the repository that have been shared over 140,000 times to over... today, we’ll start with a CRISPR overview and history. Tuesday will focus on generating knockouts using...

...first generation of base editors to the eighth generation (and dual base editors, too!).  On the seventh...

...graduate student at Harvard University, former Co-director of Science in the News, and member of the SITN...her PhD from Harvard University, is a former Co-director of Science in the News, and is a member of the...

...lab process. Along with our role as a plasmid repository, Addgene aims to be a vital educational resource...the Addgene Blog 5 Science Rap Videos and Their Creator: Tom McFadden SciComm with the Experts at Science...

...received an award from nonprofit and Addgene collaborator, Seeding Labs, for lab equipment that will help...23:44 : Follow up Kingdom's PhD projects - HIV inhibitors, biofuels, challenges in Malawi, and more 23:...

...purification Replica plating Anti-CRISPR Retroviral vectors Career development for scientists A new plasmid...do you get out of it? Writing Experience - Our editors will work with you to help hone your blog post ...

... recently sat down with Addgene Board Member, depositor, and Harvard Medical School researcher Connie ...and to get some insight into her management and mentoring styles. If you'd like to learn more about Professor...

... a functional change in the DNA sequence. Base Editors: Modified versions of CRISPR nucleases that make...sequence without completely cutting the DNA. RNA Editors: CRISPR nucleases that cut or modify RNA as opposed...

...Pathology at Masachusetts General Hospital and Postdoctoral Fellow in the Church and Collins labs at the... Blog Learn More About Alex's Work with Cas9 Activators Michael Koeris Explains His Path from Grad Student...

...introduce the newest member on Addgene’s Board of Directors, Bart Newland. Newland is General Counsel at Atalanta...really just taking off. I found out that patent attorneys with a biology background were in demand. Q: Can...

...main topics of the blog: Plasmids CRISPR Viral Vectors Fluorescent Proteins Science Careers You can use... us in the comments below. We’ll track down the story, get the inside scoop, and keep you up-to-date. ...

...its activities by facilitating connections to mentorship and networking opportunities, publishing assistance...Castano Corsino’s Recent Post on the International Mentorship Program Learn How to Network and Meet People ...

...ineffective or unimportant. With so many big contributors to the sustainability problem, how can one little... even has a list of biological samples that are stored long term at -70 °C. My Green Lab also has resources...

...from prokaryotes or eukaryotes into a synthetic vector that can have a plasmid or viral origin. The type... used during the purification procedure. Good indicators of DNA purity are the absorbance measurements...

...professional development as core components of predoctoral and postdoctoral training. A grant from the National ...

...their gene of interest into a previously deposited vector - then you'll find the plasmids listed below the...like to contribute plasmids you have made to the repository, email us and we'd be happy to help. Read More...

...Cell demonstrate how PGC-1a1, a transcriptional coactivator induced in skeletal muscle by exercise and endurance...altogether. Their muscles showed an increase in inflammatory cytokines as a result of those mild stressors...