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We narrowed to 863 results for: TOR;

Showing: 681 - 700 of 863 results

  1. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ... (LshCas13a) to begin to characterize this new effector (Abudayyeh et al., 2016). In the endogenous CRISPR...fluorophore. The fluorescent signal can be used as an indicator to determine whether the target sequence is present...single-component programmable RNA-guided RNA-targeting CRISPR effector." Science 353(6299) (2016):aaf5573. PubMed PMID...

  2. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    Published
    April 11, 2017, 2:30 p.m.
    ...on FRET techniques. By combining standard donor/acceptor FRET know-how with aptamers, aptabeacons (or aptatags...fluorophore protein. Years later, Samie Jaffrey’s laboratory took advantage of their deep knowledge of the...a higher melting temperature than its Spinach ancestors, and has a lower magnesium dependence. Though ...

  3. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...research scientists, faculty, students, private sector researchers, or pretty much anyone applying the...studies on reducing antibiotic prescriptions for respiratory tract infections. The video abstracts provide...is not hard either. Here are some resources: ImpactStory, a website that helps researchers go beyond citation...

  4. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ...CRISPR, RNAi methods are often quicker and the repositories of validated small RNAs are vast and difficult...processing Following delivery into cells using viral vectors or plasmids, shRNAs hijack the endogenous miRNA...cloning strategies for constructing shRNA expression vectors. BMC Biotechnology, 6(1). https://doi.org/10.1186...

  5. Five Popular Model Organisms

    Type
    Blog Post
    Published
    April 11, 2019, 12:39 p.m.
    ... al., 2009). By injecting mice with retroviral vectors that harbored different mutations found in leukemia...Gerald Rubin’s lab who created a set of modular vectors that can be used to optimally control gene expression...discovery of RNAi in the worm actually rewarded depositors Andrew Fire and Craig Mello a Nobel Prize in ...

  6. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    Published
    March 5, 2019, 1:08 p.m.
    ...either by electroporation of plasmids, or using AAV vectors. The Imai lab also developed a version of Tetbow...Livet, Jean, et al. "Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous...Markus Grompe. "Clonal tracing of Sox9+ liver progenitors in mouse oval cell injury." Hepatology 60.1 (...

  7. Transferable Skills Guide: Time Management

    Type
    Blog Post
    Published
    Dec. 5, 2017, 1:52 p.m.
    ...too many activities too close to one another. Monitoring task progress. For this, I always make sure I... lists. Because I know what my goals are (see monitoring task progress), and I know that I’ve blocked ... give it a final read Browse list of previous depositors from Boston University Contact Dr. Gilmore directly...

  8. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ... the microflora that reside there. Biotin is a cofactor that in its active form is covalently attached...consists of a biotin molecule attached to a photoreactive arylazide group. Under strong visible light,...single-step purification of tagged transcription factors in mammalian cells and transgenic mice.” PNAS 100.13...

  9. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    Published
    Aug. 2, 2016, 2:30 p.m.
    ...introduce or remove restriction sites), in mapping of regulatory elements (to mutate promoters/enhancers in reporter... addition to the “A” site already in the parent vector). Also shown are the “B” restriction sites, which...Browse Our Molecular Biology Protocols Find Empty Vectors for Your Next Cloning Experiment ...

  10. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    Published
    March 8, 2021, 2:41 p.m.
    ...Aydin S (2015) A short history, principles, and types of ELISA, and our laboratory experience with peptide...knockout line for validation, please view this tutorial from Stuart Orkin's and Daniel Bauer's labs.  ...

  11. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...of multiple circular DNA forms of colicinogenic factor E-1 from Proteus mirabilis. Biochemistry. 1968 ...Guttman A, Thompson L. Vaccine Plasmid Topology Monitoring by Capillary Gel Electrophoresis. Curr Mol Med... JA, Carnes AE, Hodgson CP. Plasmid DNA vaccine vector design: impact on efficacy, safety and upstream...

  12. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...modular toolkits build single plasmid targeting vectors that encode the gRNA transcript and the repair ...recognized by the 2020 Nobel Prize in Chemistry), and Victor Ambros and Gary Ruvkun for their seminal work on....114.170423 Schwartz ML, Jorgensen EM (2018) SapTrap vectors for introducing point mutations with unc-119+ selection...

  14. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    Published
    May 13, 2019, 12:01 p.m.
    ...Now we have over 8,000 CRISPR plasmids in the repository that have been shared over 140,000 times to over... today, we’ll start with a CRISPR overview and history. Tuesday will focus on generating knockouts using...

  15. The Twelve Days of CRISPR

    Type
    Blog Post
    Published
    Dec. 22, 2020, 2:15 p.m.
    ...first generation of base editors to the eighth generation (and dual base editors, too!).  On the seventh...

  16. SciComm with the Experts at Science in the News Part 1

    Type
    Blog Post
    Published
    March 16, 2017, 1:06 p.m.
    ...graduate student at Harvard University, former Co-director of Science in the News, and member of the SITN...her PhD from Harvard University, is a former Co-director of Science in the News, and is a member of the...

  17. New Educational Resource: The Addgene Videos Page

    Type
    Blog Post
    Published
    June 16, 2017, 2 p.m.
    ...lab process. Along with our role as a plasmid repository, Addgene aims to be a vital educational resource...the Addgene Blog 5 Science Rap Videos and Their Creator: Tom McFadden SciComm with the Experts at Science...

  18. Podcast: A Malawian Professor's Path to Biotech Research

    Type
    Blog Post
    Published
    May 24, 2016, 2:30 p.m.
    ...received an award from nonprofit and Addgene collaborator, Seeding Labs, for lab equipment that will help...23:44 : Follow up Kingdom's PhD projects - HIV inhibitors, biofuels, challenges in Malawi, and more 23:...

  19. Call for Guest Bloggers

    Type
    Blog Post
    Published
    March 2, 2016, 3 p.m.
    ...purification Replica plating Anti-CRISPR Retroviral vectors Career development for scientists A new plasmid...do you get out of it? Writing Experience - Our editors will work with you to help hone your blog post ...
Showing: 681 - 700 of 863 results