We narrowed to 895 results for: URE

...receiving only 1 TF expression construct. Cells were cultured following a differentiation protocol before performing...so that a single TF was delivered to each cell, future applications could deliver multiple TFs to each...on the Addgene Blog Learn More about Lentivirus Figure out How to Get the Most from Your Lentiviral Transduction...

...predict protein structures, then you might know a thing or two about computer modeling. Make sure to focus on...? Or perhaps you have presented in some public lectures? These transferable skills could be meaningful...

...see the Introduction to Fluorescence Microscopy lecture at iBiology. Commonly used filter sets & relevant...this filter set and have reported brightness measurements. Here, EGFP outperforms the improved folding...variants, presumably due to the lower growth temperature. This also suggests, however, that there is no...

...results you've measured should change established theory. So, what can you do to ensure that your skills...our website and social channels to apply for our future events. In addition to this annual event, graduate...

...processes directly affected by light. Long-term light exposure can also lead to cellular phototoxicity, and experimental...substrate like coelenterazine is supplied in the culture medium to allow RLuc8 to produce photons. The original...wavelength to specify which FP emission you’ll capture. Since the Nano-lantern system does not use external...

...virulence. Scientists have studied how HGT occurs in nature and have learned how to introduce genetic materials...Transformation, transduction, and conjugation occur in nature as forms of HGT, but transfection is unique to ...material from the environment by bacterial cells. In nature, this genetic material often comes from adjacent...

...genes for “gas vesicles,” a gas-filled protein structure that water-bound photosynthetic organisms use ... check out this blog post. Bourdeau RW, et al., Nature. 2018. PubMed PMID: 29300010. miRNA reporter vectors... date, containing all 620 sequences of probable mature human miRNAs. Weiss’s collection consists of a...

...additional flexibility in pseudotyping.   Figure 1: Genome of a prototypical gamma-retrovirus.  ...nucleus through the nuclear pore complex. This feature may be useful for the transduction of terminally...-retroviral plasmids have similar names? The nomenclature of the plasmid backbone is meant to be descriptive...

...molecules of the RNA backbone to increase stability.  Nature has evolved ways to prevent degradation of important...and rendered useless after less than a minute of exposure to the appropriate light. The photoactivatable...to engage a target sequence. Upon a brief light exposure (several seconds) the gRNA is released and the...

...consolidate several steps into one. Soon you’ll figure out what prompts you need to consistently and accurately... accurately run a protocol.   Figure 1: Evolution of a Western blot protocol, from a full page ...experimental steps are worth doing, and doing well, so make sure you’re taking time to do them carefully and thoroughly...

...screening blog post for more information.   Figure 1: Result of a blue-white screen. Image from Stefan...more about colony PCR in this blog post.   Figure 2: The steps of colony PCR.   Sanger sequencing...in this case a plasmid. Sequencing is one of the surest ways to know that your insert is what you expect...

...and you think there are four important times to measure that growth rate, you report the results of all...between hypothesis testing and generation, can be sure that results will be reported regardless of outcome...the journal and reviewers are incentivized to make sure that any possible null is really a true null and...

...won’t work if the fragment ends have secondary structure or too much sequence similarity. REPLACR-mutagenesis...expressing recombineering proteins Red/ET from a temperature-sensitive plasmid. Homologous recombination then...also well suited for insertions and deletions. The figure above shows guidelines for primer design; a desired...

...at all 1,500 or 1,600 transcription factors and figure out where they bind to the genome. We’ve been doing...other scientists quickly? Myers: We were hoping to figure out how to do this for many hundreds of transcription...as well. We would also like to put some social pressure on many other labs that may be doing something...

...Find the CASANOVA plasmids here! Bubeck F et al., Nature Methods 2018. PubMed PMID: 30377362. Studying ...the cell membrane where cell voltage can then be measured. Archon1 is  bright, exhibits good localization...processes in vivo. Many photoactive proteins exist in nature, but developing them into optogenetic tools can...

...These workshops illustrate the open and modular nature of the workshop content: different parts could ...for securing funding to support this effort. The future of reproducibility workshops and lessons learned.... Be on the lookout for upcoming workshops at future conferences this year. Workshops are confirmed ...

... known as the resting potential (Vmem), can be measured as the imbalance of electrical charge between ...of the pHLuorin fluorescent protein. This GEVI features increased brightness and improved plasma membrane...Levin, Director of the Allen Discovery Center. The future of this field has a high potential for the advancement...

..., with the expectation for major advances in agriculture, materials, chemicals, and medicine. As they’...and where all of it is likely to lead us in the future. Building circuits from CRISPRs Addgene: What...interactions. That’s relevant if you want to make sure you don’t target anywhere else, but for synthetic...

...communications and loves talking about science, nature, agriculture and bananas. You can follow her on Twitter...Plant Science today, Soapbox Science (hosted by Nature), and Addgene are open to guest contributions. ...

...increased pressure of the added liquid. The volume should be minimal in order to minimize the pressure into...mouse breeding colony) Environment Olfaction, temperature, humidity, noise level, circadian effects (inactive...